LOCUS pcDNA3_1____NDRG 5561 bp DNA circular 02-JAN-2020 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5924) AUTHORS Invitrogen TITLE Direct Submission JOURNAL Exported Monday, Dec 16, 2019 from SnapGene Viewer 5.0.6 https://www.snapgene.com COMMENT ORIGDB|GenBank COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers promoter 232..819 /locus_tag="CMV promoter" /label="CMV promoter" /ApEinfo_label="CMV promoter" /ApEinfo_fwdcolor="#346ee0" /ApEinfo_revcolor="#346ee0" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 863..879 /locus_tag="T7 promoter" /label="T7 promoter" /ApEinfo_label="T7 promoter" /ApEinfo_fwdcolor="#346ee0" /ApEinfo_revcolor="#346ee0" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" polyA_site 1578..1802 /locus_tag="BGH pA" /label="BGH pA" /ApEinfo_label="BGH pA" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="cyan" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" rep_origin 1848..2276 /direction=RIGHT /locus_tag="f1 origin" /label="f1 origin" /ApEinfo_label="f1 origin" /ApEinfo_fwdcolor="#999999" /ApEinfo_revcolor="#999999" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 966..1497 /locus_tag="NDRG1-OT1_v4 (Transcript: lnc-NDRG1-1:4)" /label="NDRG1-OT1_v4 (Transcript: lnc-NDRG1-1:4)" /ApEinfo_label="NDRG1-OT1_v4 (Transcript: lnc-NDRG1-1:4)" /ApEinfo_fwdcolor="#7eff74" /ApEinfo_revcolor="#7eff74" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" polyA_signal 3237..3367 /locus_tag="SV40 pA" /label="SV40 pA" /ApEinfo_label="SV40 pA" /ApEinfo_fwdcolor="#ff3eee" /ApEinfo_revcolor="#ff3eee" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" rep_origin complement(3750..4420) /direction=LEFT /locus_tag="pUC origin" /label="pUC origin" /ApEinfo_label="pUC origin" /ApEinfo_fwdcolor="#999999" /ApEinfo_revcolor="#999999" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS complement(4565..5425) /codon_start=1 /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY I ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY S PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR W EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS A LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA S LIKHW" /locus_tag="Amp(R)" /label="Amp(R)" /ApEinfo_label="Amp(R)" /ApEinfo_fwdcolor="#e9d024" /ApEinfo_revcolor="#e9d024" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter complement(5426..5524) /locus_tag="bla promoter" /label="bla promoter" /ApEinfo_label="bla promoter" /ApEinfo_fwdcolor="#346ee0" /ApEinfo_revcolor="#346ee0" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2667..2714 /locus_tag="EM7 promoter" /label="EM7 promoter" /ApEinfo_label="EM7 promoter" /ApEinfo_fwdcolor="#ffa262" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 939..965 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /note="HA" /translation="YPYDVPDYA" /locus_tag="HA epitope tag" /label="HA epitope tag" /ApEinfo_label="HA epitope tag" /ApEinfo_fwdcolor="#bc2129" /ApEinfo_revcolor="#2e9d36" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2733..3107 /locus_tag="BleoR" /label="BleoR" /ApEinfo_label="BleoR" /ApEinfo_fwdcolor="#ff4e6a" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1240..1240 /locus_tag="nt.275 C>G" /label="nt.275 C>G" /ApEinfo_label="nt.275 C>G" /ApEinfo_fwdcolor="#0039ff" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1079..1195 /locus_tag="putative peptide 2" /label="putative peptide 2" /ApEinfo_label="putative peptide 2" /ApEinfo_fwdcolor="#f45fff" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 1169..1192 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /note="FLAG" /translation="DYKDDDDK" /locus_tag="FLAG(R) epitope tag" /label="FLAG(R) epitope tag" /ApEinfo_label="FLAG(R) epitope tag" /ApEinfo_fwdcolor="#bc3d21" /ApEinfo_revcolor="#2e9d36" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 1250..1250 /locus_tag="nt.285 G>T" /label="nt.285 G>T" /ApEinfo_label="nt.285 G>T" /ApEinfo_fwdcolor="#0c12ff" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 2281..2650 /locus_tag="SV40 early promoter" /label="SV40 early promoter" /ApEinfo_label="SV40 early promoter" /ApEinfo_fwdcolor="#346ee0" /ApEinfo_revcolor="#346ee0" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg gatccATGTA CCCTTATGAT GTGCCAGATT 961 ATGCCGGACA AGGCAGGGCA GGGCAGCGGG CGGATGGGCA TCCTTTCGGA AAAAATTCAT 1021 AGAGCGCAGA GGGCCTTGGC TGTGGTGTTT GGCACTAACG AGCTTCCCTC TCTCCTAGAT 1081 GGCGGACTGT GGCGGCCTCC CGCAGATCTC CCAGGTTCCT GTACTACTGC CTTGTACTTT 1141 TCATTTTGGC TCACCGTGGA TTTTCTCAGA CTACAAGGAC GACGATGACA AGTAGGAAGT 1201 TTGGTCAGAG TGAATTGAAT ATTGTAAGTC AGCCACTGGg ACCCGAGGAt TTCTGGGACC 1261 CCGCAGTTGG GAGGAGGAAG TAGTCCAGCC TTCCAGGTGG CGTGAGAGGC AATGACTCGT 1321 TACCTGCCGC CCATCACCTT GGAGGCCTTC CCTGGCCTTG AGTAGAAAAG TCGGGGATCG 1381 GGGCAAGAGA GGCTGAGTAC GGATGGGAAA CTATTGTGCA CAAGTCTTTC CAGAGGAGTT 1441 TCTTAATGAG ATATTTGTAT TTATTTCCAG ACCAATAAAT TTGTAACTTT GCAGCGGgaa 1501 ttcattctgc agatatccag cacagtggcg gccgctcgag tctagagggc ccgtttaaac 1561 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc 1621 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga 1681 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga 1741 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat 1801 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag 1861 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 1921 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 1981 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca 2041 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata 2101 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 2161 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc 2221 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt 2281 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt 2341 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 2401 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 2461 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 2521 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag 2581 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata 2641 tccattttcg GATCTGATCA GCACGTGTTG ACAATTAATC ATCGGCATAG TATATCGGCA 2701 TAGTATAATA CGACAAGGTG AGGAACTAAA CCATGGCCAA GTTGACCAGT GCCGTTCCGG 2761 TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG GACCGACCGG CTCGGGTTCT 2821 CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG GGACGACGTG ACCCTGTTCA 2881 TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT GGCCTGGGTG TGGGTGCGCG 2941 GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC CACGAACTTC CGGGACGCCT 3001 CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG GCGGGAGTTC GCCCTGCGCG 3061 ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA GGACTGACAC GTGCTacgag 3121 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg 3181 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact 3241 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 3301 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 3361 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc 3421 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 3481 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 3541 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 3601 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 3661 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 3721 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 3781 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 3841 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 3901 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 3961 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 4021 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 4081 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 4141 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 4201 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg 4261 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 4321 gcaaacaaac caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa 4381 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 4441 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 4501 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 4561 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 4621 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 4681 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 4741 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 4801 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 4861 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 4921 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 4981 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 5041 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 5101 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 5161 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 5221 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 5281 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 5341 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 5401 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 5461 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 5521 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c //