• 00:00 1.
    index 1
  • 00:03 2.
    Slide 1
  • 00:35 3.
    ROS
  • 01:33 4.
    The interplay between m6A and oxidative stress
  • 02:55 5.
    Slide 4
  • 03:42 6.
    SUMOylation
  • 04:29 7.
    ROS leads to up-regulation of global mRNA m6A methylation
  • 04:31 8.
    SUMOylation
  • 04:38 9.
    ROS leads to up-regulation of global mRNA m6A methylation
  • 09:14 10.
    ROS leads to up-regulation of global mRNA m6A methylation
  • 09:55 11.
    Reducing the expression of m6A writer partially blocks ROS - induced global increase in mRNA m6A methylation
  • 10:00 12.
    ROS leads to up-regulation of global mRNA m6A methylation
  • 10:02 13.
    Reducing the expression of m6A writer partially blocks ROS - induced global increase in mRNA m6A methylation
  • 11:28 14.
    ROS promotes mRNA m6A demethylase ALKBH5 SUMOylation
  • 14:31 15.
    ALKBH5 SUMOylation blocks m6A demethylase activity by inhibition of substrate accessibility
  • 17:47 16.
    ALKBH5 SUMOylation blocks m6A demethylase activity by inhibition of substrate accessibility
  • 24:19 17.
    SUMOylation-deficient ALKBH5 overexpression blocks ROS-induced mRNA m6A methylation, leading to a significant delay of DNA repair and increase of cell apoptosis
  • 26:04 18.
    SUMOylation-deficient ALKBH5 overexpression blocks ROS-induced mRNA m6A methylation, leading to a significant delay of DNA repair and increase of cell apoptosis
  • 27:01 19.
    SUMOylation-deficient ALKBH5 overexpression blocks ROS-induced mRNA m6A methylation, leading to a significant delay of DNA repair and increase of cell apoptosis
  • 29:12 20.
    ROS induces ALKBH5 phosphorylation and SUMOylation by activation of ERK/JNK signaling
  • 32:57 21.
    ROS induces ALKBH5 phosphorylation and SUMOylation by activation of ERK/JNK signaling
  • 35:19 22.
    Global gene expression profiling identifies DNA damage repair genes as ALKBH5 downstream targets induced by ROS
  • 42:35 23.
    Global gene expression profiling identifies DNA damage repair genes as ALKBH5 downstream targets induced by ROS
  • 45:15 24.
    Global gene expression profiling identifies DNA damage repair genes as ALKBH5 downstream targets induced by ROS
  • 48:09 25.
    m6A RNA modification enhances expression of FAAP20, ATRX by IGF2BPs-mediated mRNA stabilization
  • 50:13 26.
    ROS stress response occurs in mouse bone marrow progenitor cells in vivo
  • 56:03 27.
    Slide 22
  • 57:44 28.
    ROS stress response occurs in mouse bone marrow progenitor cells in vivo
  • 57:44 29.
    m6A RNA modification enhances expression of FAAP20, ATRX by IGF2BPs-mediated mRNA stabilization
  • 57:44 30.
    Global gene expression profiling identifies DNA damage repair genes as ALKBH5 downstream targets induced by ROS
  • 57:45 31.
    Global gene expression profiling identifies DNA damage repair genes as ALKBH5 downstream targets induced by ROS
  • 57:45 32.
    Global gene expression profiling identifies DNA damage repair genes as ALKBH5 downstream targets induced by ROS
  • 57:45 33.
    ROS induces ALKBH5 phosphorylation and SUMOylation by activation of ERK/JNK signaling
  • 57:46 34.
    ROS induces ALKBH5 phosphorylation and SUMOylation by activation of ERK/JNK signaling
  • 57:46 35.
    SUMOylation-deficient ALKBH5 overexpression blocks ROS-induced mRNA m6A methylation, leading to a significant delay of DNA repair and increase of cell apoptosis
  • 57:46 36.
    SUMOylation-deficient ALKBH5 overexpression blocks ROS-induced mRNA m6A methylation, leading to a significant delay of DNA repair and increase of cell apoptosis
  • 57:47 37.
    SUMOylation-deficient ALKBH5 overexpression blocks ROS-induced mRNA m6A methylation, leading to a significant delay of DNA repair and increase of cell apoptosis
  • 57:47 38.
    ALKBH5 SUMOylation blocks m6A demethylase activity by inhibition of substrate accessibility
  • 57:48 39.
    ALKBH5 SUMOylation blocks m6A demethylase activity by inhibition of substrate accessibility
  • 57:48 40.
    ROS promotes mRNA m6A demethylase ALKBH5 SUMOylation
  • 57:48 41.
    Reducing the expression of m6A writer partially blocks ROS - induced global increase in mRNA m6A methylation
  • 57:48 42.
    ROS leads to up-regulation of global mRNA m6A methylation
  • 57:49 43.
    ROS leads to up-regulation of global mRNA m6A methylation
  • 57:49 44.
    SUMOylation
  • 57:49 45.
    Slide 4
  • 57:50 46.
    SUMOylation
  • 57:50 47.
    ROS leads to up-regulation of global mRNA m6A methylation
  • 57:52 48.
    ROS leads to up-regulation of global mRNA m6A methylation
  • 58:32 49.
    Reducing the expression of m6A writer partially blocks ROS - induced global increase in mRNA m6A methylation
  • 58:32 50.
    ROS promotes mRNA m6A demethylase ALKBH5 SUMOylation
  • 58:32 51.
    ALKBH5 SUMOylation blocks m6A demethylase activity by inhibition of substrate accessibility
  • 58:33 52.
    ALKBH5 SUMOylation blocks m6A demethylase activity by inhibition of substrate accessibility
  • 58:33 53.
    SUMOylation-deficient ALKBH5 overexpression blocks ROS-induced mRNA m6A methylation, leading to a significant delay of DNA repair and increase of cell apoptosis
  • 58:33 54.
    SUMOylation-deficient ALKBH5 overexpression blocks ROS-induced mRNA m6A methylation, leading to a significant delay of DNA repair and increase of cell apoptosis
  • 58:34 55.
    SUMOylation-deficient ALKBH5 overexpression blocks ROS-induced mRNA m6A methylation, leading to a significant delay of DNA repair and increase of cell apoptosis
  • 58:34 56.
    ROS induces ALKBH5 phosphorylation and SUMOylation by activation of ERK/JNK signaling
  • 58:34 57.
    ROS induces ALKBH5 phosphorylation and SUMOylation by activation of ERK/JNK signaling
  • 58:35 58.
    Global gene expression profiling identifies DNA damage repair genes as ALKBH5 downstream targets induced by ROS
  • 58:37 59.
    Global gene expression profiling identifies DNA damage repair genes as ALKBH5 downstream targets induced by ROS
  • 58:39 60.
    Global gene expression profiling identifies DNA damage repair genes as ALKBH5 downstream targets induced by ROS
  • 58:40 61.
    m6A RNA modification enhances expression of FAAP20, ATRX by IGF2BPs-mediated mRNA stabilization
  • 59:11 62.
    20230206 lab meeting_照穎.pptx
  • Index
  • Notes
  • Comment
  • Fullscreen
20230206 lab meeting_照穎 (no sound)
Duration: 59:34, Browse: 123, Last Updated: 2023-05-16
    • 00:00 1.
      index 1
    • 00:03 2.
      Slide 1
    • 00:35 3.
      ROS
    • 01:33 4.
      The interplay between m6A and oxidative stress
    • 02:55 5.
      Slide 4
    • 03:42 6.
      SUMOylation
    • 04:29 7.
      ROS leads to up-regulation of global mRNA m6A methylation
    • 04:31 8.
      SUMOylation
    • 04:38 9.
      ROS leads to up-regulation of global mRNA m6A methylation
    • 09:14 10.
      ROS leads to up-regulation of global mRNA m6A methylation
    • 09:55 11.
      Reducing the expression of m6A writer partially blocks ROS - induced global increase in mRNA m6A methylation
    • 10:00 12.
      ROS leads to up-regulation of global mRNA m6A methylation
    • 10:02 13.
      Reducing the expression of m6A writer partially blocks ROS - induced global increase in mRNA m6A methylation
    • 11:28 14.
      ROS promotes mRNA m6A demethylase ALKBH5 SUMOylation
    • 14:31 15.
      ALKBH5 SUMOylation blocks m6A demethylase activity by inhibition of substrate accessibility
    • 17:47 16.
      ALKBH5 SUMOylation blocks m6A demethylase activity by inhibition of substrate accessibility
    • 24:19 17.
      SUMOylation-deficient ALKBH5 overexpression blocks ROS-induced mRNA m6A methylation, leading to a significant delay of DNA repair and increase of cell apoptosis
    • 26:04 18.
      SUMOylation-deficient ALKBH5 overexpression blocks ROS-induced mRNA m6A methylation, leading to a significant delay of DNA repair and increase of cell apoptosis
    • 27:01 19.
      SUMOylation-deficient ALKBH5 overexpression blocks ROS-induced mRNA m6A methylation, leading to a significant delay of DNA repair and increase of cell apoptosis
    • 29:12 20.
      ROS induces ALKBH5 phosphorylation and SUMOylation by activation of ERK/JNK signaling
    • 32:57 21.
      ROS induces ALKBH5 phosphorylation and SUMOylation by activation of ERK/JNK signaling
    • 35:19 22.
      Global gene expression profiling identifies DNA damage repair genes as ALKBH5 downstream targets induced by ROS
    • 42:35 23.
      Global gene expression profiling identifies DNA damage repair genes as ALKBH5 downstream targets induced by ROS
    • 45:15 24.
      Global gene expression profiling identifies DNA damage repair genes as ALKBH5 downstream targets induced by ROS
    • 48:09 25.
      m6A RNA modification enhances expression of FAAP20, ATRX by IGF2BPs-mediated mRNA stabilization
    • 50:13 26.
      ROS stress response occurs in mouse bone marrow progenitor cells in vivo
    • 56:03 27.
      Slide 22
    • 57:44 28.
      ROS stress response occurs in mouse bone marrow progenitor cells in vivo
    • 57:44 29.
      m6A RNA modification enhances expression of FAAP20, ATRX by IGF2BPs-mediated mRNA stabilization
    • 57:44 30.
      Global gene expression profiling identifies DNA damage repair genes as ALKBH5 downstream targets induced by ROS
    • 57:45 31.
      Global gene expression profiling identifies DNA damage repair genes as ALKBH5 downstream targets induced by ROS
    • 57:45 32.
      Global gene expression profiling identifies DNA damage repair genes as ALKBH5 downstream targets induced by ROS
    • 57:45 33.
      ROS induces ALKBH5 phosphorylation and SUMOylation by activation of ERK/JNK signaling
    • 57:46 34.
      ROS induces ALKBH5 phosphorylation and SUMOylation by activation of ERK/JNK signaling
    • 57:46 35.
      SUMOylation-deficient ALKBH5 overexpression blocks ROS-induced mRNA m6A methylation, leading to a significant delay of DNA repair and increase of cell apoptosis
    • 57:46 36.
      SUMOylation-deficient ALKBH5 overexpression blocks ROS-induced mRNA m6A methylation, leading to a significant delay of DNA repair and increase of cell apoptosis
    • 57:47 37.
      SUMOylation-deficient ALKBH5 overexpression blocks ROS-induced mRNA m6A methylation, leading to a significant delay of DNA repair and increase of cell apoptosis
    • 57:47 38.
      ALKBH5 SUMOylation blocks m6A demethylase activity by inhibition of substrate accessibility
    • 57:48 39.
      ALKBH5 SUMOylation blocks m6A demethylase activity by inhibition of substrate accessibility
    • 57:48 40.
      ROS promotes mRNA m6A demethylase ALKBH5 SUMOylation
    • 57:48 41.
      Reducing the expression of m6A writer partially blocks ROS - induced global increase in mRNA m6A methylation
    • 57:48 42.
      ROS leads to up-regulation of global mRNA m6A methylation
    • 57:49 43.
      ROS leads to up-regulation of global mRNA m6A methylation
    • 57:49 44.
      SUMOylation
    • 57:49 45.
      Slide 4
    • 57:50 46.
      SUMOylation
    • 57:50 47.
      ROS leads to up-regulation of global mRNA m6A methylation
    • 57:52 48.
      ROS leads to up-regulation of global mRNA m6A methylation
    • 58:32 49.
      Reducing the expression of m6A writer partially blocks ROS - induced global increase in mRNA m6A methylation
    • 58:32 50.
      ROS promotes mRNA m6A demethylase ALKBH5 SUMOylation
    • 58:32 51.
      ALKBH5 SUMOylation blocks m6A demethylase activity by inhibition of substrate accessibility
    • 58:33 52.
      ALKBH5 SUMOylation blocks m6A demethylase activity by inhibition of substrate accessibility
    • 58:33 53.
      SUMOylation-deficient ALKBH5 overexpression blocks ROS-induced mRNA m6A methylation, leading to a significant delay of DNA repair and increase of cell apoptosis
    • 58:33 54.
      SUMOylation-deficient ALKBH5 overexpression blocks ROS-induced mRNA m6A methylation, leading to a significant delay of DNA repair and increase of cell apoptosis
    • 58:34 55.
      SUMOylation-deficient ALKBH5 overexpression blocks ROS-induced mRNA m6A methylation, leading to a significant delay of DNA repair and increase of cell apoptosis
    • 58:34 56.
      ROS induces ALKBH5 phosphorylation and SUMOylation by activation of ERK/JNK signaling
    • 58:34 57.
      ROS induces ALKBH5 phosphorylation and SUMOylation by activation of ERK/JNK signaling
    • 58:35 58.
      Global gene expression profiling identifies DNA damage repair genes as ALKBH5 downstream targets induced by ROS
    • 58:37 59.
      Global gene expression profiling identifies DNA damage repair genes as ALKBH5 downstream targets induced by ROS
    • 58:39 60.
      Global gene expression profiling identifies DNA damage repair genes as ALKBH5 downstream targets induced by ROS
    • 58:40 61.
      m6A RNA modification enhances expression of FAAP20, ATRX by IGF2BPs-mediated mRNA stabilization
    • 59:11 62.
      20230206 lab meeting_照穎.pptx
    Location
    Folder name
    2023
    Author
    黃照穎
    Branch
    賴亮全教授
    Created
    2023-02-06 10:28:01
    Last Updated
    2023-05-16 16:29:05
    Duration
    59:34