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00:00
1.
index 1
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00:47
2.
Introduction
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01:01
3.
Breast Cancer
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01:20
4.
Hypoxia
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01:34
5.
Hypoxia-inducible factor 1-α (HIF-1a)
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01:51
6.
Circular RNA (circRNA)
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02:17
7.
Circularization mechanism of circRNA
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02:42
8.
CircRNA circularization - Alu element
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03:08
9.
CircRNA circularization - Alu element
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03:20
10.
CircRNA circularization – RNA Binding Protein (RBP)
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03:56
11.
CircRNAs in cancer
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04:11
12.
Exosome
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04:32
13.
Nanocarriers for targeted drug delivery
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04:50
14.
Advantages of exosome as biomedical application
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05:24
15.
abundant and relatively stableremodel the tumor microenvironment
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05:47
16.
Potential clinical applications of exosomal circRNAs in cancer therapy
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06:02
17.
Introduction
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06:05
18.
Hypoxia-induced circRNA-circSFMBT2
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06:47
19.
CircSFMBT2 acted as a tumor suppressor in breast cancer
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07:09
20.
Slide 20
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07:12
21.
Slide 21
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07:48
22.
Slide 22
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08:07
23.
The expression level of pre-SFMBT2, exon2-4, exon5-7, exon8-10 upregulated during hypoxia
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08:43
24.
Hypothesis
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09:05
25.
The expression of circSFMBT2 enhanced after HIF-1α overexpression
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09:26
26.
Prediction of HRE on SFMBT2 promoter
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09:42
27.
HIF1a bound at HRE1 and HRE3 on SFMBT2 promoter
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10:12
28.
HIF-1α promoted the transcription of SFMBT2 through HRE1 and HRE3
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10:48
29.
Summary
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11:05
30.
Slide 30
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11:11
31.
CircRNA circularization
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11:28
32.
Existence of Alu elements in intron4 and intron7 of pre-SFMBT2
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11:49
33.
Circularization of circSFMBT2 was partially through Alu elements
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12:32
34.
The RNA level of QKI upregulated under hypoxia
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13:13
35.
The protein level of QKI downregulated under hypoxia
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13:30
36.
Screening RBPs through mass spectrometry
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14:03
37.
DDX54 bound at intron4 and intron7 of pre-SFMBT2
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14:19
38.
Prediction of DDX54 binding sites on pre-SFMBT2
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14:34
39.
DDX54 bound on pre-SFMBT2
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14:44
40.
DDX54 (DEAD box RNA helicase)
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15:15
41.
The expression of DDX54 downregulated under hypoxia
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15:33
42.
Hypothesis
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15:54
43.
The expression of circSFMBT2 upregulated after DDX54 knockdown
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16:14
44.
Summary
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16:51
45.
Slide 45
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16:56
46.
Characterizations of exosomes
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18:11
47.
CircSFMBT2 downregulated in breast cancer tissues and different breast cancer cell lines
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18:36
48.
Slide 48
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18:53
49.
The expression of exo-circSFMBT2 both increased under hypoxia and after circSFMBT2 overexpression
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19:18
50.
The expression of intracellular circSFMBT2 upregulated after circSFMBT2 exos treatment
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19:52
51.
Slide 51
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19:55
52.
Conclusions
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20:39
53.
Conclusions
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21:01
54.
Slide 54
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21:04
55.
Q1
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21:17
56.
<40 nt of intronic inverted repeats are sufficient to trigger circularization
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21:42
57.
A high complementary region of sequences between AluY and AluSc5
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21:51
58.
Future work
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22:08
59.
Q2
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22:34
60.
Both QKI mRNA and protein levels were markedly reduced in breast cancer tissues
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22:58
61.
Future work
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23:08
62.
Q3
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23:37
63.
Different exosome isolation methods
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24:30
64.
Different exosome quantification methods
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24:43
65.
Bicinchoninic Acid (BCA) assay was used in this study
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25:16
66.
Low circSFMBT2 packaging rate
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25:34
67.
CircRHOBTB3 was sorted into exosomes by interacting with SNF8
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26:01
68.
Future work
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26:30
69.
Slide 69
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27:00
70.
** after 口試.pptx
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36:41
71.
Characterizations of exosomes
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37:20
72.
CircSFMBT2 downregulated in breast cancer tissues and different breast cancer cell lines
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37:22
73.
Slide 48
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37:24
74.
The expression of exo-circSFMBT2 both increased under hypoxia and after circSFMBT2 overexpression
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37:24
75.
The expression of intracellular circSFMBT2 upregulated after circSFMBT2 exos treatment
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37:25
76.
Slide 51
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37:25
77.
Conclusions
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37:26
78.
Conclusions
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38:30
79.
** after 口試.pptx
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38:47
80.
Characterizations of exosomes
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38:52
81.
CircSFMBT2 downregulated in breast cancer tissues and different breast cancer cell lines
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38:53
82.
Slide 48
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38:55
83.
The expression of exo-circSFMBT2 both increased under hypoxia and after circSFMBT2 overexpression
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39:05
84.
The expression of intracellular circSFMBT2 upregulated after circSFMBT2 exos treatment
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39:06
85.
Slide 51
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39:06
86.
Conclusions
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39:07
87.
Conclusions
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39:07
88.
Slide 54
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39:08
89.
Q1
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39:09
90.
<40 nt of intronic inverted repeats are sufficient to trigger circularization
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39:09
91.
A high complementary region of sequences between AluY and AluSc5
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39:09
92.
Future work
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39:10
93.
Q2
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39:10
94.
Both QKI mRNA and protein levels were markedly reduced in breast cancer tissues
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39:10
95.
Future work
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39:11
96.
Q3
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39:11
97.
Different exosome isolation methods
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39:11
98.
Different exosome quantification methods
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39:11
99.
Bicinchoninic Acid (BCA) assay was used in this study
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39:12
100.
Low circSFMBT2 packaging rate
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39:12
101.
CircRHOBTB3 was sorted into exosomes by interacting with SNF8
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39:12
102.
Future work
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39:13
103.
Slide 69
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39:13
104.
Slide 70
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39:13
105.
Slide 71
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39:14
106.
Slide 72
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39:14
107.
Slide 73
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39:14
108.
Slide 74
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39:15
109.
The expression of intracellular circSFMBT2 did not change after hypoxic exos treatment
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39:47
110.
** after 口試.pptx
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39:58
111.
The expression of exo-circSFMBT2 both increased under hypoxia and after circSFMBT2 overexpression
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40:43
112.
** after 口試.pptx
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40:54
113.
Different exosome quantification methods
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41:48
114.
Bicinchoninic Acid (BCA) assay was used in this study
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44:18
115.
Different exosome quantification methods
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44:18
116.
Different exosome isolation methods
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44:19
117.
Q3
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44:19
118.
Future work
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44:19
119.
Both QKI mRNA and protein levels were markedly reduced in breast cancer tissues
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44:19
120.
Q2
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44:20
121.
Future work
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44:21
122.
A high complementary region of sequences between AluY and AluSc5
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44:21
123.
<40 nt of intronic inverted repeats are sufficient to trigger circularization
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44:21
124.
Q1
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44:22
125.
Slide 54
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44:23
126.
Conclusions
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44:23
127.
Conclusions
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44:23
128.
Slide 51
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44:24
129.
The expression of intracellular circSFMBT2 upregulated after circSFMBT2 exos treatment
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44:24
130.
The expression of exo-circSFMBT2 both increased under hypoxia and after circSFMBT2 overexpression
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44:24
131.
Slide 48
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44:25
132.
The expression of exo-circSFMBT2 both increased under hypoxia and after circSFMBT2 overexpression
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44:26
133.
The expression of intracellular circSFMBT2 upregulated after circSFMBT2 exos treatment
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45:20
134.
** after 口試.pptx
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45:29
135.
The expression level of pre-SFMBT2, exon2-4, exon5-7, exon8-10 upregulated during hypoxia
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46:08
136.
Hypothesis
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46:08
137.
The expression of circSFMBT2 enhanced after HIF-1α overexpression
-
46:08
138.
Prediction of HRE on SFMBT2 promoter
-
46:09
139.
HIF1a bound at HRE1 and HRE3 on SFMBT2 promoter
-
46:10
140.
HIF-1α promoted the transcription of SFMBT2 through HRE1 and HRE3
-
46:10
141.
Summary
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46:11
142.
Slide 30
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46:12
143.
CircRNA circularization
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46:13
144.
Existence of Alu elements in intron4 and intron7 of pre-SFMBT2
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46:13
145.
Circularization of circSFMBT2 was partially through Alu elements
-
46:13
146.
The RNA level of QKI upregulated under hypoxia
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46:56
147.
** after 口試.pptx
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47:04
148.
Conclusions
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48:25
149.
** after 口試.pptx
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48:39
150.
Prediction of DDX54 binding sites on pre-SFMBT2
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50:17
151.
DDX54 bound at intron4 and intron7 of pre-SFMBT2
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50:18
152.
Screening RBPs through mass spectrometry
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50:18
153.
The protein level of QKI downregulated under hypoxia
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50:19
154.
Screening RBPs through mass spectrometry
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52:40
155.
** after 口試.pptx
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57:18
156.
Characterizations of exosomes
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58:04
157.
** after 口試.pptx
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1:00:23
158.
Slide 70
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1:01:14
159.
Slide 69
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1:01:14
160.
Future work
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1:01:15
161.
CircRHOBTB3 was sorted into exosomes by interacting with SNF8
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1:01:16
162.
Low circSFMBT2 packaging rate
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1:01:16
163.
Bicinchoninic Acid (BCA) assay was used in this study
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1:01:16
164.
Different exosome quantification methods
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1:01:17
165.
Different exosome isolation methods
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1:01:17
166.
Q3
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1:01:18
167.
Future work
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1:01:18
168.
Both QKI mRNA and protein levels were markedly reduced in breast cancer tissues
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1:01:18
169.
Q2
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1:01:19
170.
Future work
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1:01:19
171.
A high complementary region of sequences between AluY and AluSc5
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1:01:20
172.
<40 nt of intronic inverted repeats are sufficient to trigger circularization
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1:01:33
173.
** after 口試.pptx
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1:01:41
174.
Existence of Alu elements in intron4 and intron7 of pre-SFMBT2
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1:02:35
175.
** after 口試.pptx
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1:02:45
176.
Slide 71
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1:03:39
177.
** after 口試.pptx