• 00:00 1.
    index 1
  • 00:47 2.
    Introduction
  • 01:01 3.
    Breast Cancer
  • 01:20 4.
    Hypoxia
  • 01:34 5.
    Hypoxia-inducible factor 1-α (HIF-1a)
  • 01:51 6.
    Circular RNA (circRNA)
  • 02:17 7.
    Circularization mechanism of circRNA
  • 02:42 8.
    CircRNA circularization - Alu element
  • 03:08 9.
    CircRNA circularization - Alu element
  • 03:20 10.
    CircRNA circularization – RNA Binding Protein (RBP)
  • 03:56 11.
    CircRNAs in cancer
  • 04:11 12.
    Exosome
  • 04:32 13.
    Nanocarriers for targeted drug delivery
  • 04:50 14.
    Advantages of exosome as biomedical application
  • 05:24 15.
    abundant and relatively stableremodel the tumor microenvironment
  • 05:47 16.
    Potential clinical applications of exosomal circRNAs in cancer therapy
  • 06:02 17.
    Introduction
  • 06:05 18.
    Hypoxia-induced circRNA-circSFMBT2
  • 06:47 19.
    CircSFMBT2 acted as a tumor suppressor in breast cancer
  • 07:09 20.
    Slide 20
  • 07:12 21.
    Slide 21
  • 07:48 22.
    Slide 22
  • 08:07 23.
    The expression level of pre-SFMBT2, exon2-4, exon5-7, exon8-10 upregulated during hypoxia
  • 08:43 24.
    Hypothesis
  • 09:05 25.
    The expression of circSFMBT2 enhanced after HIF-1α overexpression
  • 09:26 26.
    Prediction of HRE on SFMBT2 promoter
  • 09:42 27.
    HIF1a bound at HRE1 and HRE3 on SFMBT2 promoter
  • 10:12 28.
    HIF-1α promoted the transcription of SFMBT2 through HRE1 and HRE3
  • 10:48 29.
    Summary
  • 11:05 30.
    Slide 30
  • 11:11 31.
    CircRNA circularization
  • 11:28 32.
    Existence of Alu elements in intron4 and intron7 of pre-SFMBT2
  • 11:49 33.
    Circularization of circSFMBT2 was partially through Alu elements
  • 12:32 34.
    The RNA level of QKI upregulated under hypoxia
  • 13:13 35.
    The protein level of QKI downregulated under hypoxia
  • 13:30 36.
    Screening RBPs through mass spectrometry
  • 14:03 37.
    DDX54 bound at intron4 and intron7 of pre-SFMBT2
  • 14:19 38.
    Prediction of DDX54 binding sites on pre-SFMBT2
  • 14:34 39.
    DDX54 bound on pre-SFMBT2
  • 14:44 40.
    DDX54 (DEAD box RNA helicase)
  • 15:15 41.
    The expression of DDX54 downregulated under hypoxia
  • 15:33 42.
    Hypothesis
  • 15:54 43.
    The expression of circSFMBT2 upregulated after DDX54 knockdown
  • 16:14 44.
    Summary
  • 16:51 45.
    Slide 45
  • 16:56 46.
    Characterizations of exosomes
  • 18:11 47.
    CircSFMBT2 downregulated in breast cancer tissues and different breast cancer cell lines
  • 18:36 48.
    Slide 48
  • 18:53 49.
    The expression of exo-circSFMBT2 both increased under hypoxia and after circSFMBT2 overexpression
  • 19:18 50.
    The expression of intracellular circSFMBT2 upregulated after circSFMBT2 exos treatment
  • 19:52 51.
    Slide 51
  • 19:55 52.
    Conclusions
  • 20:39 53.
    Conclusions
  • 21:01 54.
    Slide 54
  • 21:04 55.
    Q1
  • 21:17 56.
    <40 nt of intronic inverted repeats are sufficient to trigger circularization
  • 21:42 57.
    A high complementary region of sequences between AluY and AluSc5
  • 21:51 58.
    Future work
  • 22:08 59.
    Q2
  • 22:34 60.
    Both QKI mRNA and protein levels were markedly reduced in breast cancer tissues
  • 22:58 61.
    Future work
  • 23:08 62.
    Q3
  • 23:37 63.
    Different exosome isolation methods
  • 24:30 64.
    Different exosome quantification methods
  • 24:43 65.
    Bicinchoninic Acid (BCA) assay was used in this study
  • 25:16 66.
    Low circSFMBT2 packaging rate
  • 25:34 67.
    CircRHOBTB3 was sorted into exosomes by interacting with SNF8
  • 26:01 68.
    Future work
  • 26:30 69.
    Slide 69
  • 27:00 70.
    ** after 口試.pptx
  • 36:41 71.
    Characterizations of exosomes
  • 37:20 72.
    CircSFMBT2 downregulated in breast cancer tissues and different breast cancer cell lines
  • 37:22 73.
    Slide 48
  • 37:24 74.
    The expression of exo-circSFMBT2 both increased under hypoxia and after circSFMBT2 overexpression
  • 37:24 75.
    The expression of intracellular circSFMBT2 upregulated after circSFMBT2 exos treatment
  • 37:25 76.
    Slide 51
  • 37:25 77.
    Conclusions
  • 37:26 78.
    Conclusions
  • 38:30 79.
    ** after 口試.pptx
  • 38:47 80.
    Characterizations of exosomes
  • 38:52 81.
    CircSFMBT2 downregulated in breast cancer tissues and different breast cancer cell lines
  • 38:53 82.
    Slide 48
  • 38:55 83.
    The expression of exo-circSFMBT2 both increased under hypoxia and after circSFMBT2 overexpression
  • 39:05 84.
    The expression of intracellular circSFMBT2 upregulated after circSFMBT2 exos treatment
  • 39:06 85.
    Slide 51
  • 39:06 86.
    Conclusions
  • 39:07 87.
    Conclusions
  • 39:07 88.
    Slide 54
  • 39:08 89.
    Q1
  • 39:09 90.
    <40 nt of intronic inverted repeats are sufficient to trigger circularization
  • 39:09 91.
    A high complementary region of sequences between AluY and AluSc5
  • 39:09 92.
    Future work
  • 39:10 93.
    Q2
  • 39:10 94.
    Both QKI mRNA and protein levels were markedly reduced in breast cancer tissues
  • 39:10 95.
    Future work
  • 39:11 96.
    Q3
  • 39:11 97.
    Different exosome isolation methods
  • 39:11 98.
    Different exosome quantification methods
  • 39:11 99.
    Bicinchoninic Acid (BCA) assay was used in this study
  • 39:12 100.
    Low circSFMBT2 packaging rate
  • 39:12 101.
    CircRHOBTB3 was sorted into exosomes by interacting with SNF8
  • 39:12 102.
    Future work
  • 39:13 103.
    Slide 69
  • 39:13 104.
    Slide 70
  • 39:13 105.
    Slide 71
  • 39:14 106.
    Slide 72
  • 39:14 107.
    Slide 73
  • 39:14 108.
    Slide 74
  • 39:15 109.
    The expression of intracellular circSFMBT2 did not change after hypoxic exos treatment
  • 39:47 110.
    ** after 口試.pptx
  • 39:58 111.
    The expression of exo-circSFMBT2 both increased under hypoxia and after circSFMBT2 overexpression
  • 40:43 112.
    ** after 口試.pptx
  • 40:54 113.
    Different exosome quantification methods
  • 41:48 114.
    Bicinchoninic Acid (BCA) assay was used in this study
  • 44:18 115.
    Different exosome quantification methods
  • 44:18 116.
    Different exosome isolation methods
  • 44:19 117.
    Q3
  • 44:19 118.
    Future work
  • 44:19 119.
    Both QKI mRNA and protein levels were markedly reduced in breast cancer tissues
  • 44:19 120.
    Q2
  • 44:20 121.
    Future work
  • 44:21 122.
    A high complementary region of sequences between AluY and AluSc5
  • 44:21 123.
    <40 nt of intronic inverted repeats are sufficient to trigger circularization
  • 44:21 124.
    Q1
  • 44:22 125.
    Slide 54
  • 44:23 126.
    Conclusions
  • 44:23 127.
    Conclusions
  • 44:23 128.
    Slide 51
  • 44:24 129.
    The expression of intracellular circSFMBT2 upregulated after circSFMBT2 exos treatment
  • 44:24 130.
    The expression of exo-circSFMBT2 both increased under hypoxia and after circSFMBT2 overexpression
  • 44:24 131.
    Slide 48
  • 44:25 132.
    The expression of exo-circSFMBT2 both increased under hypoxia and after circSFMBT2 overexpression
  • 44:26 133.
    The expression of intracellular circSFMBT2 upregulated after circSFMBT2 exos treatment
  • 45:20 134.
    ** after 口試.pptx
  • 45:29 135.
    The expression level of pre-SFMBT2, exon2-4, exon5-7, exon8-10 upregulated during hypoxia
  • 46:08 136.
    Hypothesis
  • 46:08 137.
    The expression of circSFMBT2 enhanced after HIF-1α overexpression
  • 46:08 138.
    Prediction of HRE on SFMBT2 promoter
  • 46:09 139.
    HIF1a bound at HRE1 and HRE3 on SFMBT2 promoter
  • 46:10 140.
    HIF-1α promoted the transcription of SFMBT2 through HRE1 and HRE3
  • 46:10 141.
    Summary
  • 46:11 142.
    Slide 30
  • 46:12 143.
    CircRNA circularization
  • 46:13 144.
    Existence of Alu elements in intron4 and intron7 of pre-SFMBT2
  • 46:13 145.
    Circularization of circSFMBT2 was partially through Alu elements
  • 46:13 146.
    The RNA level of QKI upregulated under hypoxia
  • 46:56 147.
    ** after 口試.pptx
  • 47:04 148.
    Conclusions
  • 48:25 149.
    ** after 口試.pptx
  • 48:39 150.
    Prediction of DDX54 binding sites on pre-SFMBT2
  • 50:17 151.
    DDX54 bound at intron4 and intron7 of pre-SFMBT2
  • 50:18 152.
    Screening RBPs through mass spectrometry
  • 50:18 153.
    The protein level of QKI downregulated under hypoxia
  • 50:19 154.
    Screening RBPs through mass spectrometry
  • 52:40 155.
    ** after 口試.pptx
  • 57:18 156.
    Characterizations of exosomes
  • 58:04 157.
    ** after 口試.pptx
  • 1:00:23 158.
    Slide 70
  • 1:01:14 159.
    Slide 69
  • 1:01:14 160.
    Future work
  • 1:01:15 161.
    CircRHOBTB3 was sorted into exosomes by interacting with SNF8
  • 1:01:16 162.
    Low circSFMBT2 packaging rate
  • 1:01:16 163.
    Bicinchoninic Acid (BCA) assay was used in this study
  • 1:01:16 164.
    Different exosome quantification methods
  • 1:01:17 165.
    Different exosome isolation methods
  • 1:01:17 166.
    Q3
  • 1:01:18 167.
    Future work
  • 1:01:18 168.
    Both QKI mRNA and protein levels were markedly reduced in breast cancer tissues
  • 1:01:18 169.
    Q2
  • 1:01:19 170.
    Future work
  • 1:01:19 171.
    A high complementary region of sequences between AluY and AluSc5
  • 1:01:20 172.
    <40 nt of intronic inverted repeats are sufficient to trigger circularization
  • 1:01:33 173.
    ** after 口試.pptx
  • 1:01:41 174.
    Existence of Alu elements in intron4 and intron7 of pre-SFMBT2
  • 1:02:35 175.
    ** after 口試.pptx
  • 1:02:45 176.
    Slide 71
  • 1:03:39 177.
    ** after 口試.pptx
  • 索引
  • 筆記
  • 討論
  • 全螢幕
20231023 lab meeting_怡君
長度: 1:06:30, 瀏覽: 233, 最近修訂: 2023-10-23
    上一篇 下一篇
    • 00:00 1.
      index 1
    • 00:47 2.
      Introduction
    • 01:01 3.
      Breast Cancer
    • 01:20 4.
      Hypoxia
    • 01:34 5.
      Hypoxia-inducible factor 1-α (HIF-1a)
    • 01:51 6.
      Circular RNA (circRNA)
    • 02:17 7.
      Circularization mechanism of circRNA
    • 02:42 8.
      CircRNA circularization - Alu element
    • 03:08 9.
      CircRNA circularization - Alu element
    • 03:20 10.
      CircRNA circularization – RNA Binding Protein (RBP)
    • 03:56 11.
      CircRNAs in cancer
    • 04:11 12.
      Exosome
    • 04:32 13.
      Nanocarriers for targeted drug delivery
    • 04:50 14.
      Advantages of exosome as biomedical application
    • 05:24 15.
      abundant and relatively stableremodel the tumor microenvironment
    • 05:47 16.
      Potential clinical applications of exosomal circRNAs in cancer therapy
    • 06:02 17.
      Introduction
    • 06:05 18.
      Hypoxia-induced circRNA-circSFMBT2
    • 06:47 19.
      CircSFMBT2 acted as a tumor suppressor in breast cancer
    • 07:09 20.
      Slide 20
    • 07:12 21.
      Slide 21
    • 07:48 22.
      Slide 22
    • 08:07 23.
      The expression level of pre-SFMBT2, exon2-4, exon5-7, exon8-10 upregulated during hypoxia
    • 08:43 24.
      Hypothesis
    • 09:05 25.
      The expression of circSFMBT2 enhanced after HIF-1α overexpression
    • 09:26 26.
      Prediction of HRE on SFMBT2 promoter
    • 09:42 27.
      HIF1a bound at HRE1 and HRE3 on SFMBT2 promoter
    • 10:12 28.
      HIF-1α promoted the transcription of SFMBT2 through HRE1 and HRE3
    • 10:48 29.
      Summary
    • 11:05 30.
      Slide 30
    • 11:11 31.
      CircRNA circularization
    • 11:28 32.
      Existence of Alu elements in intron4 and intron7 of pre-SFMBT2
    • 11:49 33.
      Circularization of circSFMBT2 was partially through Alu elements
    • 12:32 34.
      The RNA level of QKI upregulated under hypoxia
    • 13:13 35.
      The protein level of QKI downregulated under hypoxia
    • 13:30 36.
      Screening RBPs through mass spectrometry
    • 14:03 37.
      DDX54 bound at intron4 and intron7 of pre-SFMBT2
    • 14:19 38.
      Prediction of DDX54 binding sites on pre-SFMBT2
    • 14:34 39.
      DDX54 bound on pre-SFMBT2
    • 14:44 40.
      DDX54 (DEAD box RNA helicase)
    • 15:15 41.
      The expression of DDX54 downregulated under hypoxia
    • 15:33 42.
      Hypothesis
    • 15:54 43.
      The expression of circSFMBT2 upregulated after DDX54 knockdown
    • 16:14 44.
      Summary
    • 16:51 45.
      Slide 45
    • 16:56 46.
      Characterizations of exosomes
    • 18:11 47.
      CircSFMBT2 downregulated in breast cancer tissues and different breast cancer cell lines
    • 18:36 48.
      Slide 48
    • 18:53 49.
      The expression of exo-circSFMBT2 both increased under hypoxia and after circSFMBT2 overexpression
    • 19:18 50.
      The expression of intracellular circSFMBT2 upregulated after circSFMBT2 exos treatment
    • 19:52 51.
      Slide 51
    • 19:55 52.
      Conclusions
    • 20:39 53.
      Conclusions
    • 21:01 54.
      Slide 54
    • 21:04 55.
      Q1
    • 21:17 56.
      <40 nt of intronic inverted repeats are sufficient to trigger circularization
    • 21:42 57.
      A high complementary region of sequences between AluY and AluSc5
    • 21:51 58.
      Future work
    • 22:08 59.
      Q2
    • 22:34 60.
      Both QKI mRNA and protein levels were markedly reduced in breast cancer tissues
    • 22:58 61.
      Future work
    • 23:08 62.
      Q3
    • 23:37 63.
      Different exosome isolation methods
    • 24:30 64.
      Different exosome quantification methods
    • 24:43 65.
      Bicinchoninic Acid (BCA) assay was used in this study
    • 25:16 66.
      Low circSFMBT2 packaging rate
    • 25:34 67.
      CircRHOBTB3 was sorted into exosomes by interacting with SNF8
    • 26:01 68.
      Future work
    • 26:30 69.
      Slide 69
    • 27:00 70.
      ** after 口試.pptx
    • 36:41 71.
      Characterizations of exosomes
    • 37:20 72.
      CircSFMBT2 downregulated in breast cancer tissues and different breast cancer cell lines
    • 37:22 73.
      Slide 48
    • 37:24 74.
      The expression of exo-circSFMBT2 both increased under hypoxia and after circSFMBT2 overexpression
    • 37:24 75.
      The expression of intracellular circSFMBT2 upregulated after circSFMBT2 exos treatment
    • 37:25 76.
      Slide 51
    • 37:25 77.
      Conclusions
    • 37:26 78.
      Conclusions
    • 38:30 79.
      ** after 口試.pptx
    • 38:47 80.
      Characterizations of exosomes
    • 38:52 81.
      CircSFMBT2 downregulated in breast cancer tissues and different breast cancer cell lines
    • 38:53 82.
      Slide 48
    • 38:55 83.
      The expression of exo-circSFMBT2 both increased under hypoxia and after circSFMBT2 overexpression
    • 39:05 84.
      The expression of intracellular circSFMBT2 upregulated after circSFMBT2 exos treatment
    • 39:06 85.
      Slide 51
    • 39:06 86.
      Conclusions
    • 39:07 87.
      Conclusions
    • 39:07 88.
      Slide 54
    • 39:08 89.
      Q1
    • 39:09 90.
      <40 nt of intronic inverted repeats are sufficient to trigger circularization
    • 39:09 91.
      A high complementary region of sequences between AluY and AluSc5
    • 39:09 92.
      Future work
    • 39:10 93.
      Q2
    • 39:10 94.
      Both QKI mRNA and protein levels were markedly reduced in breast cancer tissues
    • 39:10 95.
      Future work
    • 39:11 96.
      Q3
    • 39:11 97.
      Different exosome isolation methods
    • 39:11 98.
      Different exosome quantification methods
    • 39:11 99.
      Bicinchoninic Acid (BCA) assay was used in this study
    • 39:12 100.
      Low circSFMBT2 packaging rate
    • 39:12 101.
      CircRHOBTB3 was sorted into exosomes by interacting with SNF8
    • 39:12 102.
      Future work
    • 39:13 103.
      Slide 69
    • 39:13 104.
      Slide 70
    • 39:13 105.
      Slide 71
    • 39:14 106.
      Slide 72
    • 39:14 107.
      Slide 73
    • 39:14 108.
      Slide 74
    • 39:15 109.
      The expression of intracellular circSFMBT2 did not change after hypoxic exos treatment
    • 39:47 110.
      ** after 口試.pptx
    • 39:58 111.
      The expression of exo-circSFMBT2 both increased under hypoxia and after circSFMBT2 overexpression
    • 40:43 112.
      ** after 口試.pptx
    • 40:54 113.
      Different exosome quantification methods
    • 41:48 114.
      Bicinchoninic Acid (BCA) assay was used in this study
    • 44:18 115.
      Different exosome quantification methods
    • 44:18 116.
      Different exosome isolation methods
    • 44:19 117.
      Q3
    • 44:19 118.
      Future work
    • 44:19 119.
      Both QKI mRNA and protein levels were markedly reduced in breast cancer tissues
    • 44:19 120.
      Q2
    • 44:20 121.
      Future work
    • 44:21 122.
      A high complementary region of sequences between AluY and AluSc5
    • 44:21 123.
      <40 nt of intronic inverted repeats are sufficient to trigger circularization
    • 44:21 124.
      Q1
    • 44:22 125.
      Slide 54
    • 44:23 126.
      Conclusions
    • 44:23 127.
      Conclusions
    • 44:23 128.
      Slide 51
    • 44:24 129.
      The expression of intracellular circSFMBT2 upregulated after circSFMBT2 exos treatment
    • 44:24 130.
      The expression of exo-circSFMBT2 both increased under hypoxia and after circSFMBT2 overexpression
    • 44:24 131.
      Slide 48
    • 44:25 132.
      The expression of exo-circSFMBT2 both increased under hypoxia and after circSFMBT2 overexpression
    • 44:26 133.
      The expression of intracellular circSFMBT2 upregulated after circSFMBT2 exos treatment
    • 45:20 134.
      ** after 口試.pptx
    • 45:29 135.
      The expression level of pre-SFMBT2, exon2-4, exon5-7, exon8-10 upregulated during hypoxia
    • 46:08 136.
      Hypothesis
    • 46:08 137.
      The expression of circSFMBT2 enhanced after HIF-1α overexpression
    • 46:08 138.
      Prediction of HRE on SFMBT2 promoter
    • 46:09 139.
      HIF1a bound at HRE1 and HRE3 on SFMBT2 promoter
    • 46:10 140.
      HIF-1α promoted the transcription of SFMBT2 through HRE1 and HRE3
    • 46:10 141.
      Summary
    • 46:11 142.
      Slide 30
    • 46:12 143.
      CircRNA circularization
    • 46:13 144.
      Existence of Alu elements in intron4 and intron7 of pre-SFMBT2
    • 46:13 145.
      Circularization of circSFMBT2 was partially through Alu elements
    • 46:13 146.
      The RNA level of QKI upregulated under hypoxia
    • 46:56 147.
      ** after 口試.pptx
    • 47:04 148.
      Conclusions
    • 48:25 149.
      ** after 口試.pptx
    • 48:39 150.
      Prediction of DDX54 binding sites on pre-SFMBT2
    • 50:17 151.
      DDX54 bound at intron4 and intron7 of pre-SFMBT2
    • 50:18 152.
      Screening RBPs through mass spectrometry
    • 50:18 153.
      The protein level of QKI downregulated under hypoxia
    • 50:19 154.
      Screening RBPs through mass spectrometry
    • 52:40 155.
      ** after 口試.pptx
    • 57:18 156.
      Characterizations of exosomes
    • 58:04 157.
      ** after 口試.pptx
    • 1:00:23 158.
      Slide 70
    • 1:01:14 159.
      Slide 69
    • 1:01:14 160.
      Future work
    • 1:01:15 161.
      CircRHOBTB3 was sorted into exosomes by interacting with SNF8
    • 1:01:16 162.
      Low circSFMBT2 packaging rate
    • 1:01:16 163.
      Bicinchoninic Acid (BCA) assay was used in this study
    • 1:01:16 164.
      Different exosome quantification methods
    • 1:01:17 165.
      Different exosome isolation methods
    • 1:01:17 166.
      Q3
    • 1:01:18 167.
      Future work
    • 1:01:18 168.
      Both QKI mRNA and protein levels were markedly reduced in breast cancer tissues
    • 1:01:18 169.
      Q2
    • 1:01:19 170.
      Future work
    • 1:01:19 171.
      A high complementary region of sequences between AluY and AluSc5
    • 1:01:20 172.
      <40 nt of intronic inverted repeats are sufficient to trigger circularization
    • 1:01:33 173.
      ** after 口試.pptx
    • 1:01:41 174.
      Existence of Alu elements in intron4 and intron7 of pre-SFMBT2
    • 1:02:35 175.
      ** after 口試.pptx
    • 1:02:45 176.
      Slide 71
    • 1:03:39 177.
      ** after 口試.pptx
    位置
    1. 知識庫
    2. ...
    3. 2023
    資料夾名稱
    2023
    發表人
    周楷軒
    單位
    賴亮全教授
    建立
    2023-10-23 10:17:18
    最近修訂
    2023-10-23 10:41:20
    長度
    1:06:30

    臺北市仁愛路一段1號10樓1009室

    R1009 10F No1 Sec1 Ren-Ai Rd, Taipei 100233, Taiwan.

    E-mail: llai@ntu.edu.tw

    Tel: 886-2-2312-3456 # 288241

    Lab: 886-2-2393-8235