• 00:00 1.
    index 1
  • 00:28 2.
    Slide 2
  • 00:29 3.
    HBV-related hepatocellular carcinoma
  • 02:15 4.
    HBV induces protein O-GlcNAcylation in liver cancer
  • 03:29 5.
    Hexosamine Biosynthetic Pathway (O-GlcNAcylation)
  • 05:00 6.
    RNA N6-methyladenosine (m6A) Modification
  • 06:18 7.
    Slide 7
  • 06:42 8.
    Slide 8
  • 07:47 9.
    HBV infection enhanced YTHDF2 O-GlcNAcylation in vivo and clinical
  • 07:50 10.
    Slide 8
  • 07:58 11.
    HBV infection enhanced YTHDF2 O-GlcNAcylation in vivo and clinical
  • 10:31 12.
    HBV infection enhanced YTHDF2 O-GlcNAcylation in vitro
  • 12:58 13.
    OGT interacted with the N-terminal region of YTHDF2
  • 23:00 14.
    Ser263 is the major O-GlcNAcylation site of YTHDF2
  • 29:00 15.
    YTHDF2 protein was more stable after HBV infection in HCCKnockdown of OGT greatly shortened YTHDF2 lifespan
  • 34:57 16.
    TMG treatment could not restorereducing YTHDF2 half-life by S263A- YTHDF2 mutant
  • 35:39 17.
    Slide 15
  • 35:40 18.
    TMG treatment could not restorereducing YTHDF2 half-life by S263A- YTHDF2 mutant
  • 38:36 19.
    Slide 15
  • 47:39 20.
    O-GlcNAcylation of YTHDF2 promotes hepatocellular carcinoma cell proliferation, invasion and migration in vitro
  • 49:57 21.
    O-GlcNAcylation of YTHDF2 promotes tumor growth in vivo
  • 50:37 22.
    Identification of YTHDF2 targets by RNA-seq, m6A-seq and RIP-seq
  • 58:19 23.
    O-GlcNAcylation of YTHDF2 increased MCM2/5 mRNA stability in an m6A-dependent manner
  • 1:06:02 24.
    MCM2/5 promotes HCC proliferation by O-GlcNAcylation of YTHDF2
  • 1:08:24 25.
    Slide 21
  • 1:10:38 26.
    Slide 22
  • 1:11:46 27.
    Slide 23
  • 1:12:37 28.
    Slide 24
  • 1:12:38 29.
    Conclusion
  • 1:13:33 30.
    Comments
  • 1:13:37 31.
    Conclusion
  • 1:14:02 32.
    Comments
  • 1:14:42 33.
    Slide 27
  • 1:15:11 34.
    ** after 20231030 meeting.pptx
  • Index
  • Notes
  • Comment
  • Fullscreen
20231030 lab meeting_楷軒
Duration: 1:15:17, Browse: 61, Last Updated: 2023-10-30
    • 00:00 1.
      index 1
    • 00:28 2.
      Slide 2
    • 00:29 3.
      HBV-related hepatocellular carcinoma
    • 02:15 4.
      HBV induces protein O-GlcNAcylation in liver cancer
    • 03:29 5.
      Hexosamine Biosynthetic Pathway (O-GlcNAcylation)
    • 05:00 6.
      RNA N6-methyladenosine (m6A) Modification
    • 06:18 7.
      Slide 7
    • 06:42 8.
      Slide 8
    • 07:47 9.
      HBV infection enhanced YTHDF2 O-GlcNAcylation in vivo and clinical
    • 07:50 10.
      Slide 8
    • 07:58 11.
      HBV infection enhanced YTHDF2 O-GlcNAcylation in vivo and clinical
    • 10:31 12.
      HBV infection enhanced YTHDF2 O-GlcNAcylation in vitro
    • 12:58 13.
      OGT interacted with the N-terminal region of YTHDF2
    • 23:00 14.
      Ser263 is the major O-GlcNAcylation site of YTHDF2
    • 29:00 15.
      YTHDF2 protein was more stable after HBV infection in HCCKnockdown of OGT greatly shortened YTHDF2 lifespan
    • 34:57 16.
      TMG treatment could not restorereducing YTHDF2 half-life by S263A- YTHDF2 mutant
    • 35:39 17.
      Slide 15
    • 35:40 18.
      TMG treatment could not restorereducing YTHDF2 half-life by S263A- YTHDF2 mutant
    • 38:36 19.
      Slide 15
    • 47:39 20.
      O-GlcNAcylation of YTHDF2 promotes hepatocellular carcinoma cell proliferation, invasion and migration in vitro
    • 49:57 21.
      O-GlcNAcylation of YTHDF2 promotes tumor growth in vivo
    • 50:37 22.
      Identification of YTHDF2 targets by RNA-seq, m6A-seq and RIP-seq
    • 58:19 23.
      O-GlcNAcylation of YTHDF2 increased MCM2/5 mRNA stability in an m6A-dependent manner
    • 1:06:02 24.
      MCM2/5 promotes HCC proliferation by O-GlcNAcylation of YTHDF2
    • 1:08:24 25.
      Slide 21
    • 1:10:38 26.
      Slide 22
    • 1:11:46 27.
      Slide 23
    • 1:12:37 28.
      Slide 24
    • 1:12:38 29.
      Conclusion
    • 1:13:33 30.
      Comments
    • 1:13:37 31.
      Conclusion
    • 1:14:02 32.
      Comments
    • 1:14:42 33.
      Slide 27
    • 1:15:11 34.
      ** after 20231030 meeting.pptx
    Location
    Folder name
    2023
    Author
    周楷軒
    Branch
    賴亮全教授
    Created
    2023-10-30 10:47:27
    Last Updated
    2023-10-30 11:17:48
    Duration
    1:15:17