• 00:00 1.
    Sterile Cell Culture Technique
  • 01:12 2.
    Clean cabinet wiith 70% Ethanol
  • 03:16 3.
    Remove culture medium
  • 03:52 4.
    Wash cells
  • 04:34 5.
    Add trypsin
  • 05:24 5.1
    incubate at 37C 3-4 min
  • 06:46 6.
    Harvest cells
  • 07:56 6.1
    Spin cells at 1800 RPM for 4 min
  • 08:49 7.
    Transfer to new flask
  • Index
  • Notes
  • Comment
Sterile Cell Culture Technique 細胞培養無菌技術
Duration: 13:23, Browse: 2095, Last Updated: 2018-12-22
Demonstration of sterile animal cell culture technique developed for the University of Manchester, School of Materials.
    • 00:00 1.
      Sterile Cell Culture Technique
    • 01:12 2.
      Clean cabinet wiith 70% Ethanol
    • 03:16 3.
      Remove culture medium
    • 03:52 4.
      Wash cells
    • 04:34 5.
      Add trypsin
    • 05:24 5.1
      incubate at 37C 3-4 min
    • 06:46 6.
      Harvest cells
    • 07:56 6.1
      Spin cells at 1800 RPM for 4 min
    • 08:49 7.
      Transfer to new flask
    Location
    Folder name
    細胞培養 (Cell culture)
    Author
    賴亮全
    Branch
    賴亮全教授
    Created
    2018-12-22 13:39:00
    Last Updated
    2018-12-22 15:05:50
    Duration
    13:23
    Reference
    1
    Source
    https://www.youtube.com/watch?v=ugcGo42VNqI