MTT

  1. Transfect完的細胞seed到96 well plate(每個well 3000個cell),overnight使其貼附
  2. 第0天做第一次: 倒掉medium,加入MTT 100 ul(稀釋10) (incubate for 2 hrs),倒掉MTT,加DMSO 100ul 5 min (都需避光,間隔24 hr)
  3. Elisa reader測570 nm吸光值
    Location
    Folder name
    MTT assay(忠賢)
    Author
    石忠賢
    Branch
    賴亮全教授
    Label
    MTT
    Created
    2019-01-07 01:34:55
    Last Updated
    2019-01-07 01:43:33