Migration Assay
-
00:00
1.
Migration Assay
-
00:05
2.
Put migration chambers i 24-well plate
-
00:30
3.
Add 100ul serum-free medium in the chamber
-
00:48
4.
Add 200ul cell (2.5 x 10^5/ml in serum-free medium) in the chamber
-
01:12
5.
Add 750ul culture medium (WITH serum) in lower chamber
-
01:46
5.1
Incubate at 37C for 12-16 hours
-
01:51
6.
Remove medium from the chamber
-
02:03
7.
Wash twice in PBS
-
02:10
8.
Fix cells by formaldehyde (3.7% in PBS)
-
02:35
8.1
Fix cells at room temp. for 2 min
-
02:39
9.
Remove formaldehyde and wash twice by PBS
-
03:13
10.
Permeabilize cells by 100% methanol
-
03:35
10.1
Permeabilization at room temp for 20 min
-
03:40
11.
Remove methanol and wash twice by PBS
-
04:07
12.
Giemsa stain
-
04:41
13.
Remove Giemsa stain and wash twice by PBS
-
05:05
14.
Scrape off non-migrated cells with cotton swabs
-
05:21
15.
Count migrated cells under a light microscope
播放影片: http://llai.cm.ntu.edu.tw/media/402
( http://www.abnova.com ) - The migration assay (also known as the Boyden Chamber Assay) is a commonly used test to study the migratory response of endothelial cells. During this assay, cells are placed on the upper layer of a chamber and a solution containing the test agent is placed below the chamber.
-
00:00
1.
Migration Assay
-
00:05
2.
Put migration chambers i 24-well plate
-
00:30
3.
Add 100ul serum-free medium in the chamber
-
00:48
4.
Add 200ul cell (2.5 x 10^5/ml in serum-free medium) in the chamber
-
01:12
5.
Add 750ul culture medium (WITH serum) in lower chamber
-
01:46
5.1
Incubate at 37C for 12-16 hours
-
01:51
6.
Remove medium from the chamber
-
02:03
7.
Wash twice in PBS
-
02:10
8.
Fix cells by formaldehyde (3.7% in PBS)
-
02:35
8.1
Fix cells at room temp. for 2 min
-
02:39
9.
Remove formaldehyde and wash twice by PBS
-
03:13
10.
Permeabilize cells by 100% methanol
-
03:35
10.1
Permeabilization at room temp for 20 min
-
03:40
11.
Remove methanol and wash twice by PBS
-
04:07
12.
Giemsa stain
-
04:41
13.
Remove Giemsa stain and wash twice by PBS
-
05:05
14.
Scrape off non-migrated cells with cotton swabs
-
05:21
15.
Count migrated cells under a light microscope
- 位置
-
- 資料夾名稱
- migration assay (Abnova版本; 伊純)
- 發表人
- 鄭伊純
- 單位
- 賴亮全教授
- 建立
- 2019-01-07 08:16:33
- 最近修訂
- 2019-01-07 08:16:34
- 長度
- 05:36
- 來源
- https://youtu.be/6SON7VAA5-k