• 00:00 1.
    Migration Assay
  • 00:05 2.
    Put migration chambers i 24-well plate
  • 00:30 3.
    Add 100ul serum-free medium in the chamber
  • 00:48 4.
    Add 200ul cell (2.5 x 10^5/ml in serum-free medium) in the chamber
  • 01:12 5.
    Add 750ul culture medium (WITH serum) in lower chamber
  • 01:46 5.1
    Incubate at 37C for 12-16 hours
  • 01:51 6.
    Remove medium from the chamber
  • 02:03 7.
    Wash twice in PBS
  • 02:10 8.
    Fix cells by formaldehyde (3.7% in PBS)
  • 02:35 8.1
    Fix cells at room temp. for 2 min
  • 02:39 9.
    Remove formaldehyde and wash twice by PBS
  • 03:13 10.
    Permeabilize cells by 100% methanol
  • 03:35 10.1
    Permeabilization at room temp for 20 min
  • 03:40 11.
    Remove methanol and wash twice by PBS
  • 04:07 12.
    Giemsa stain
  • 04:41 13.
    Remove Giemsa stain and wash twice by PBS
  • 05:05 14.
    Scrape off non-migrated cells with cotton swabs
  • 05:21 15.
    Count migrated cells under a light microscope
  • Index
  • Notes
  • Comment
Migration Assay
Duration: 05:36, Browse: 1480, Last Updated: 2019-01-07
( http://www.abnova.com ) - The migration assay (also known as the Boyden Chamber Assay) is a commonly used test to study the migratory response of endothelial cells. During this assay, cells are placed on the upper layer of a chamber and a solution containing the test agent is placed below the chamber.
    • 00:00 1.
      Migration Assay
    • 00:05 2.
      Put migration chambers i 24-well plate
    • 00:30 3.
      Add 100ul serum-free medium in the chamber
    • 00:48 4.
      Add 200ul cell (2.5 x 10^5/ml in serum-free medium) in the chamber
    • 01:12 5.
      Add 750ul culture medium (WITH serum) in lower chamber
    • 01:46 5.1
      Incubate at 37C for 12-16 hours
    • 01:51 6.
      Remove medium from the chamber
    • 02:03 7.
      Wash twice in PBS
    • 02:10 8.
      Fix cells by formaldehyde (3.7% in PBS)
    • 02:35 8.1
      Fix cells at room temp. for 2 min
    • 02:39 9.
      Remove formaldehyde and wash twice by PBS
    • 03:13 10.
      Permeabilize cells by 100% methanol
    • 03:35 10.1
      Permeabilization at room temp for 20 min
    • 03:40 11.
      Remove methanol and wash twice by PBS
    • 04:07 12.
      Giemsa stain
    • 04:41 13.
      Remove Giemsa stain and wash twice by PBS
    • 05:05 14.
      Scrape off non-migrated cells with cotton swabs
    • 05:21 15.
      Count migrated cells under a light microscope
    Location
    Folder name
    migration assay (Abnova版本; 伊純)
    Author
    鄭伊純
    Branch
    賴亮全教授
    Created
    2019-01-07 08:16:33
    Last Updated
    2019-01-07 08:16:34
    Duration
    05:36
    Source
    https://youtu.be/6SON7VAA5-k