• 00:00 1.
    How to use Dynabeads® for immunoprecipitation
  • Index
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How to use Dynabeads® for immunoprecipitation
Duration: 05:11, Browse: 1008, Last Updated: 2019-01-07
Learn more: http://www.lifetechnologies.com/immunoprecipitation This video shows the 3 simple protocol steps + some tips & tricks: - Bead preparations & protocol scaling - Ab choice (type &concentration) - How to use the magnet - Pipetting & mixing - Crosslinking (optional) - Direct vs. indirect binding - Elution You can jump between chapters by clicking the top bar.
Keypoint
  1. 1.
    延伸:Co-IP
     
    問題:如何確定你的 target protein 與其他 protein 的結合為專一性結合,而非實驗誤差 binding 到與 target protein 無交互作用的 protein?
    Location
    Folder name
    IP (immunoprecipitation) 捷登
    Author
    鄭捷登
    Branch
    賴亮全教授
    Created
    2019-01-07 12:52:44
    Last Updated
    2019-01-07 12:56:40
    Duration
    05:11
    Source
    https://www.youtube.com/watch?v=FMMlDMzJnwE