RNA Extraction by TRIzol® | 賴亮全實驗室

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00:00 1.
RNA Extraction by TRIzol®
00:07 2.
Add 1ml TRIzol to the sample and homogenize
00:27 3.
Add 200ul chloroform to the homogenate
00:38 4.
Vortex vigorously
00:42 5.
Incubate on ice for 15min
00:46 6.
Centrifuge to get phase separation (12000g for 15min at 4C)
00:58 7.
Transfer the aqueous phase to a fresh tube
01:24 8.
Precipitate the RNA by mixing with 0.5ml isopropanol
01:26 9.
Incubate on ice 10min
01:40 10.
Centrifuge for 10 min at 12000g at 4C
01:54 11.
Remove the supernatant
02:01 12.
Wash pellet with 1ml 70% ethanol by flicking
02:17 13.
Centrifuge at 7500g for 10min at 4C
02:30 14.
Remove the supernatant
02:39 15.
Air dry
02:45 16.
Dissolve RNA pellet in appropriate vol of RNase-free H2O

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00:00 1.
RNA Extraction by TRIzol®
00:07 2.
Add 1ml TRIzol to the sample and homogenize
00:27 3.
Add 200ul chloroform to the homogenate
00:38 4.
Vortex vigorously
00:42 5.
Incubate on ice for 15min
00:46 6.
Centrifuge to get phase separation (12000g for 15min at 4C)
00:58 7.
Transfer the aqueous phase to a fresh tube
01:24 8.
Precipitate the RNA by mixing with 0.5ml isopropanol
01:26 9.
Incubate on ice 10min
01:40 10.
Centrifuge for 10 min at 12000g at 4C
01:54 11.
Remove the supernatant
02:01 12.
Wash pellet with 1ml 70% ethanol by flicking
02:17 13.
Centrifuge at 7500g for 10min at 4C
02:30 14.
Remove the supernatant
02:39 15.
Air dry
02:45 16.
Dissolve RNA pellet in appropriate vol of RNase-free H2O

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RNA Extraction by TRIzol®

長度: 03:05, 瀏覽: 4173, 最近修訂: 2019-01-13

( http://www.abnova.com ) - TRIzol® is a mono-phasic solution of phenol and guanidine isothiocyanate. It is a ready-to-use reagent for the isolation of total RNA from cells and tissues. After addition of TRIzol® and chloroform, phase separation is created by centrifugation. RNA is present in the aqueous phase and can be recovered by precipitation with isopropanol or ethanol.

來源 : https://www.youtube.com/watch?v=RmPsLoIPRwc