• 00:00 1.
    RNA Extraction by TRIzol®
  • 00:07 2.
    Add 1ml TRIzol to the sample and homogenize
  • 00:27 3.
    Add 200ul chloroform to the homogenate
  • 00:38 4.
    Vortex vigorously
  • 00:42 5.
    Incubate on ice for 15min
  • 00:46 6.
    Centrifuge to get phase separation (12000g for 15min at 4C)
  • 00:58 7.
    Transfer the aqueous phase to a fresh tube
  • 01:24 8.
    Precipitate the RNA by mixing with 0.5ml isopropanol
  • 01:26 9.
    Incubate on ice 10min
  • 01:40 10.
    Centrifuge for 10 min at 12000g at 4C
  • 01:54 11.
    Remove the supernatant
  • 02:01 12.
    Wash pellet with 1ml 70% ethanol by flicking
  • 02:17 13.
    Centrifuge at 7500g for 10min at 4C
  • 02:30 14.
    Remove the supernatant
  • 02:39 15.
    Air dry
  • 02:45 16.
    Dissolve RNA pellet in appropriate vol of RNase-free H2O
  • Index
  • Notes
  • Comment
RNA Extraction by TRIzol®
Duration: 03:05, Browse: 4240, Last Updated: 2019-01-13
( http://www.abnova.com ) - TRIzol® is a mono-phasic solution of phenol and guanidine isothiocyanate. It is a ready-to-use reagent for the isolation of total RNA from cells and tissues. After addition of TRIzol® and chloroform, phase separation is created by centrifugation. RNA is present in the aqueous phase and can be recovered by precipitation with isopropanol or ethanol.
    • 00:00 1.
      RNA Extraction by TRIzol®
    • 00:07 2.
      Add 1ml TRIzol to the sample and homogenize
    • 00:27 3.
      Add 200ul chloroform to the homogenate
    • 00:38 4.
      Vortex vigorously
    • 00:42 5.
      Incubate on ice for 15min
    • 00:46 6.
      Centrifuge to get phase separation (12000g for 15min at 4C)
    • 00:58 7.
      Transfer the aqueous phase to a fresh tube
    • 01:24 8.
      Precipitate the RNA by mixing with 0.5ml isopropanol
    • 01:26 9.
      Incubate on ice 10min
    • 01:40 10.
      Centrifuge for 10 min at 12000g at 4C
    • 01:54 11.
      Remove the supernatant
    • 02:01 12.
      Wash pellet with 1ml 70% ethanol by flicking
    • 02:17 13.
      Centrifuge at 7500g for 10min at 4C
    • 02:30 14.
      Remove the supernatant
    • 02:39 15.
      Air dry
    • 02:45 16.
      Dissolve RNA pellet in appropriate vol of RNase-free H2O
    Location
    Folder name
    DNA/RNA extraction
    Author
    賴亮全
    Branch
    賴亮全教授
    Created
    2019-01-13 21:35:34
    Last Updated
    2019-01-13 21:35:35
    Duration
    03:05
    Source
    https://www.youtube.com/watch?v=RmPsLoIPRwc