Protocol 1 - DNA Extraction Part 1
-
00:00
1.
RNA Extraction by TRIzol®
-
00:07
2.
Add 1ml TRIzol to the sample and homogenize
-
00:27
3.
Add 200ul chloroform to the homogenate
-
00:38
4.
Vortex vigorously
-
00:42
5.
Incubate on ice for 15min
-
00:46
6.
Centrifuge to get phase separation (12000g for 15min at 4C)
-
00:58
7.
Transfer the aqueous phase to a fresh tube
-
01:24
8.
Precipitate the RNA by mixing with 0.5ml isopropanol
-
01:26
9.
Incubate on ice 10min
-
01:40
10.
Centrifuge for 10 min at 12000g at 4C
-
01:54
11.
Remove the supernatant
-
02:01
12.
Wash pellet with 1ml 70% ethanol by flicking
-
02:17
13.
Centrifuge at 7500g for 10min at 4C
-
02:30
14.
Remove the supernatant
-
02:39
15.
Air dry
-
02:45
16.
Dissolve RNA pellet in appropriate vol of RNase-free H2O
- Index
- Notes
- Comment
RNA Extraction by TRIzol®
Duration: 03:05, Browse: 4795, Last Updated: 2019-01-13
Play Video: http://llai.cm.ntu.edu.tw/media/462
( http://www.abnova.com ) - TRIzol® is a mono-phasic solution of phenol and guanidine isothiocyanate. It is a ready-to-use reagent for the isolation of total RNA from cells and tissues. After addition of TRIzol® and chloroform, phase separation is created by centrifugation. RNA is present in the aqueous phase and can be recovered by precipitation with isopropanol or ethanol.
-
00:00
1.
RNA Extraction by TRIzol®
-
00:07
2.
Add 1ml TRIzol to the sample and homogenize
-
00:27
3.
Add 200ul chloroform to the homogenate
-
00:38
4.
Vortex vigorously
-
00:42
5.
Incubate on ice for 15min
-
00:46
6.
Centrifuge to get phase separation (12000g for 15min at 4C)
-
00:58
7.
Transfer the aqueous phase to a fresh tube
-
01:24
8.
Precipitate the RNA by mixing with 0.5ml isopropanol
-
01:26
9.
Incubate on ice 10min
-
01:40
10.
Centrifuge for 10 min at 12000g at 4C
-
01:54
11.
Remove the supernatant
-
02:01
12.
Wash pellet with 1ml 70% ethanol by flicking
-
02:17
13.
Centrifuge at 7500g for 10min at 4C
-
02:30
14.
Remove the supernatant
-
02:39
15.
Air dry
-
02:45
16.
Dissolve RNA pellet in appropriate vol of RNase-free H2O
- Location
-
- Folder name
- DNA/RNA extraction
- Author
- 賴亮全
- Branch
- 賴亮全教授
- Created
- 2019-01-13 21:35:34
- Last Updated
- 2019-01-13 21:35:35
- Duration
- 03:05
- Source
- https://www.youtube.com/watch?v=RmPsLoIPRwc