Easy DNA transfection with jetPRIME - Polyplus-transfection

00:00 1.
Easy DNA transfection with jetPRIME - Polyplus-transfection

Share
- Embed URL
- QR code

Index
Notes
Comment

Duration: 02:41, Browse: 826, Last Updated: 2019-01-23

jetPRIME® is a powerful and versatile DNA and siRNA transfection reagent for day-to-day experiments that leads to efficient and reliable scientific results. jetPRIME® ensures high DNA transfection efficiency and excellent gene silencing in a variety of adherent cells. jetPRIME ® is also ideal for DNA/siRNA co-transfection or co-delivery of several plasmids.

Source : https://www.youtube.com/watch?time_continue=24&v=f1R6gdB9XXY

Keypoint

1.

jetPRIME的用量標準
不同格式的dish會有不同的jetPRIME用量及DNA用量，請參考以下用量建議：
2.
Transfection 時期的細胞培養
1. 建議當細胞貼壁且生長良好後 (貼壁通常需4小時，最好overnight) 才進行轉染（實際上生長勢強的細胞株也可以在懸浮時加入jetPRIME，可節省時間)

2. 加入 jetPRIME 後，通常是24小時後可抽RNA，protein則為48小時後抽。以上時間估計可自行先做過不同時間點後觀察最佳的轉染時間長度。