• 00:00 1.
    Easy DNA transfection with jetPRIME - Polyplus-transfection
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Easy DNA transfection with jetPRIME - Polyplus-transfection
Duration: 02:41, Browse: 1018, Last Updated: 2019-01-23
jetPRIME® is a powerful and versatile DNA and siRNA transfection reagent for day-to-day experiments that leads to efficient and reliable scientific results. jetPRIME® ensures high DNA transfection efficiency and excellent gene silencing in a variety of adherent cells. jetPRIME ® is also ideal for DNA/siRNA co-transfection or co-delivery of several plasmids.
Keypoint
  1. 1.
    jetPRIME的用量標準
    不同格式的dish會有不同的jetPRIME用量及DNA用量,請參考以下用量建議:
     
  2. 2.
    Transfection 時期的細胞培養
    1. 建議當細胞貼壁且生長良好後 (貼壁通常需4小時,最好overnight) 才進行轉染(實際上生長勢強的細胞株也可以在懸浮時加入jetPRIME,可節省時間)
     
    2. 加入 jetPRIME 後,通常是24小時後可抽RNA,protein則為48小時後抽。以上時間估計可自行先做過不同時間點後觀察最佳的轉染時間長度。
    Location
    Folder name
    Transfection (轉染)
    Author
    鄭捷登
    Branch
    賴亮全教授
    Created
    2019-01-14 08:27:29
    Last Updated
    2019-01-23 14:52:27
    Duration
    02:41
    Source
    https://www.youtube.com/watch?time_continue=24&v=f1R6gdB9XXY