Invasion Assay
-
00:00
1.
Invasion Assay
-
00:06
2.
Put transwell chambers in 24-well plate
-
00:25
3.
Prepare Matrigel
-
00:48
3.1
Add 100 ul Matrigel in the chamber
-
01:09
4.
Incubate at 37 C overnight for gelling
-
01:15
5.
Add 750 ul culture medium with serum in the lower chamber
-
01:26
6.
Place 200 ul cell (2.5x10^5/ml in serum-free medium) in upper chamber
-
01:51
7.
Place transwell into lower chamber
-
02:09
8.
Incubate at 37 C for 12-16 h
-
02:13
9.
Remove medium and wash twice by PBS
-
02:52
10.
Fix cells by formaldehyde (3.7% in PBS)
-
03:17
10.1
Fix cells at room temp for 2 min
-
03:20
11.
Remove formaldehyde and wash twice by PBS
-
03:55
12.
Permeabilize cells by 100% methanol
-
04:16
12.1
Permeabilization at room temp for 20 min
-
04:18
13.
Remove methanol and wash twice by PBS
-
04:46
14.
Giemsa stain
-
05:10
14.1
Giemsa stain at room temp for 15 min
-
05:15
14.2
Remove Giemsa stain and wash twice by PBS
-
05:43
15.
Scrape off non-invasive cells with cotton swabs
-
06:04
16.
Count invasive cells under a light microscope
播放影片: http://llai.cm.ntu.edu.tw/media/67
-
00:00
1.
Invasion Assay
-
00:06
2.
Put transwell chambers in 24-well plate
-
00:25
3.
Prepare Matrigel
-
00:48
3.1
Add 100 ul Matrigel in the chamber
-
01:09
4.
Incubate at 37 C overnight for gelling
-
01:15
5.
Add 750 ul culture medium with serum in the lower chamber
-
01:26
6.
Place 200 ul cell (2.5x10^5/ml in serum-free medium) in upper chamber
-
01:51
7.
Place transwell into lower chamber
-
02:09
8.
Incubate at 37 C for 12-16 h
-
02:13
9.
Remove medium and wash twice by PBS
-
02:52
10.
Fix cells by formaldehyde (3.7% in PBS)
-
03:17
10.1
Fix cells at room temp for 2 min
-
03:20
11.
Remove formaldehyde and wash twice by PBS
-
03:55
12.
Permeabilize cells by 100% methanol
-
04:16
12.1
Permeabilization at room temp for 20 min
-
04:18
13.
Remove methanol and wash twice by PBS
-
04:46
14.
Giemsa stain
-
05:10
14.1
Giemsa stain at room temp for 15 min
-
05:15
14.2
Remove Giemsa stain and wash twice by PBS
-
05:43
15.
Scrape off non-invasive cells with cotton swabs
-
06:04
16.
Count invasive cells under a light microscope
- 位置
-
- 資料夾名稱
- Invasion assay (Abnova 版本)
- 發表人
- 系統管理者
- 單位
- 賴亮全教授
- 建立
- 2018-12-03 11:19:48
- 最近修訂
- 2018-12-03 11:26:14
- 長度
- 06:17