Invasion Assay | 賴亮全實驗室

Index

00:00 1.
Invasion Assay
00:06 2.
Put transwell chambers in 24-well plate
00:25 3.
Prepare Matrigel
00:48 3.1
Add 100 ul Matrigel in the chamber
01:09 4.
Incubate at 37 C overnight for gelling
01:15 5.
Add 750 ul culture medium with serum in the lower chamber
01:26 6.
Place 200 ul cell (2.5x10^5/ml in serum-free medium) in upper chamber
01:51 7.
Place transwell into lower chamber
02:09 8.
Incubate at 37 C for 12-16 h
02:13 9.
Remove medium and wash twice by PBS
02:52 10.
Fix cells by formaldehyde (3.7% in PBS)
03:17 10.1
Fix cells at room temp for 2 min
03:20 11.
Remove formaldehyde and wash twice by PBS
03:55 12.
Permeabilize cells by 100% methanol
04:16 12.1
Permeabilization at room temp for 20 min
04:18 13.
Remove methanol and wash twice by PBS
04:46 14.
Giemsa stain
05:10 14.1
Giemsa stain at room temp for 15 min
05:15 14.2
Remove Giemsa stain and wash twice by PBS
05:43 15.
Scrape off non-invasive cells with cotton swabs
06:04 16.
Count invasive cells under a light microscope

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00:00 1.
Invasion Assay
00:06 2.
Put transwell chambers in 24-well plate
00:25 3.
Prepare Matrigel
00:48 3.1
Add 100 ul Matrigel in the chamber
01:09 4.
Incubate at 37 C overnight for gelling
01:15 5.
Add 750 ul culture medium with serum in the lower chamber
01:26 6.
Place 200 ul cell (2.5x10^5/ml in serum-free medium) in upper chamber
01:51 7.
Place transwell into lower chamber
02:09 8.
Incubate at 37 C for 12-16 h
02:13 9.
Remove medium and wash twice by PBS
02:52 10.
Fix cells by formaldehyde (3.7% in PBS)
03:17 10.1
Fix cells at room temp for 2 min
03:20 11.
Remove formaldehyde and wash twice by PBS
03:55 12.
Permeabilize cells by 100% methanol
04:16 12.1
Permeabilization at room temp for 20 min
04:18 13.
Remove methanol and wash twice by PBS
04:46 14.
Giemsa stain
05:10 14.1
Giemsa stain at room temp for 15 min
05:15 14.2
Remove Giemsa stain and wash twice by PBS
05:43 15.
Scrape off non-invasive cells with cotton swabs
06:04 16.
Count invasive cells under a light microscope

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Invasion Assay

Duration: 06:17, Browse: 1035, Last Updated: 2018-12-03

00:00 1.
Invasion Assay
00:06 2.
Put transwell chambers in 24-well plate
00:25 3.
Prepare Matrigel
00:48 3.1
Add 100 ul Matrigel in the chamber
01:09 4.
Incubate at 37 C overnight for gelling
01:15 5.
Add 750 ul culture medium with serum in the lower chamber
01:26 6.
Place 200 ul cell (2.5x10^5/ml in serum-free medium) in upper chamber
01:51 7.
Place transwell into lower chamber
02:09 8.
Incubate at 37 C for 12-16 h
02:13 9.
Remove medium and wash twice by PBS
02:52 10.
Fix cells by formaldehyde (3.7% in PBS)
03:17 10.1
Fix cells at room temp for 2 min
03:20 11.
Remove formaldehyde and wash twice by PBS
03:55 12.
Permeabilize cells by 100% methanol
04:16 12.1
Permeabilization at room temp for 20 min
04:18 13.
Remove methanol and wash twice by PBS
04:46 14.
Giemsa stain
05:10 14.1
Giemsa stain at room temp for 15 min
05:15 14.2
Remove Giemsa stain and wash twice by PBS
05:43 15.
Scrape off non-invasive cells with cotton swabs
06:04 16.
Count invasive cells under a light microscope

Location

Folder name

Invasion assay (Abnova 版本)

Author

系統管理者

Branch

賴亮全教授

Created

2018-12-03 11:19:48

Last Updated

2018-12-03 11:26:14

Duration

06:17