Fix cells
methanol: acetic acid=3:1
[現行protocol] Incubate 10 min
-
00:33
1.
Clonogenic Assay
-
00:46
2.
Wash with 1x PBS
-
00:23
3.
Fix cells (methanol: acetic acid=3:1)
-
00:04
4.
Incubate 5 min
-
00:26
5.
Remove the fixing reagent
-
00:25
6.
Stain cells in 0.5% crystal violet (in methanol) for 15 min
-
00:12
7.
wash by tap water
-
00:06
8.
Count the colonies (>50 cells in a colony)
- Index
- Notes
- Comment
- Fullscreen
Clonogenic Assay
Duration: 02:58, Browse: 3290, Last Updated: 2018-12-26
Play Video: http://llai.cm.ntu.edu.tw/media/70
Keypoint
- 1.
- 2.Stain cells[現行protocol] Stain cells in 0.1% crystal violet (in methanol) for 10 min0.5% crystal violet (in methanol) for 15 min
-
00:33
1.
Clonogenic Assay
-
00:46
2.
Wash with 1x PBS
-
00:23
3.
Fix cells (methanol: acetic acid=3:1)
-
00:04
4.
Incubate 5 min
-
00:26
5.
Remove the fixing reagent
-
00:25
6.
Stain cells in 0.5% crystal violet (in methanol) for 15 min
-
00:12
7.
wash by tap water
-
00:06
8.
Count the colonies (>50 cells in a colony)
- Location
-
- Folder name
- Clonogenic assay (Abnova版本)
- Author
- 系統管理者
- Branch
- 賴亮全教授
- Created
- 2018-12-03 11:21:32
- Last Updated
- 2018-12-26 16:08:33
- Browse
- 3,290
- Duration
- 02:58