Fix cells
methanol: acetic acid=3:1
[現行protocol] Incubate 10 min
-
00:00
1.
Clonogenic Assay
-
00:33
2.
Wash with 1x PBS
-
01:19
3.
Fix cells (methanol: acetic acid=3:1)
-
01:43
4.
Incubate 5 min
-
01:47
5.
Remove the fixing reagent
-
02:13
6.
Stain cells in 0.5% crystal violet (in methanol) for 15 min
-
02:39
7.
wash by tap water
-
02:51
8.
Count the colonies (>50 cells in a colony)
播放影片: http://llai.cm.ntu.edu.tw/media/70
重點
- 1.
- 2.Stain cells[現行protocol] Stain cells in 0.1% crystal violet (in methanol) for 10 min0.5% crystal violet (in methanol) for 15 min
-
00:00
1.
Clonogenic Assay
-
00:33
2.
Wash with 1x PBS
-
01:19
3.
Fix cells (methanol: acetic acid=3:1)
-
01:43
4.
Incubate 5 min
-
01:47
5.
Remove the fixing reagent
-
02:13
6.
Stain cells in 0.5% crystal violet (in methanol) for 15 min
-
02:39
7.
wash by tap water
-
02:51
8.
Count the colonies (>50 cells in a colony)
- 位置
-
- 資料夾名稱
- Clonogenic assay (Abnova版本)
- 發表人
- 系統管理者
- 單位
- 賴亮全教授
- 建立
- 2018-12-03 11:21:32
- 最近修訂
- 2018-12-26 16:08:33
- 長度
- 02:58