• 00:00 1.
    Clonogenic Assay
  • 00:33 2.
    Wash with 1x PBS
  • 01:19 3.
    Fix cells (methanol: acetic acid=3:1)
  • 01:43 4.
    Incubate 5 min
  • 01:47 5.
    Remove the fixing reagent
  • 02:13 6.
    Stain cells in 0.5% crystal violet (in methanol) for 15 min
  • 02:39 7.
    wash by tap water
  • 02:51 8.
    Count the colonies (>50 cells in a colony)
  • Index
  • Notes
  • Comment
  • Fullscreen
Clonogenic Assay
Duration: 02:58, Browse: 3552, Last Updated: 2018-12-26
Keypoint
  1. 1.
    Fix cells
    methanol: acetic acid=3:1
    [現行protocol] Incubate 10 min
  2. 2.
    Stain cells
    [現行protocol] Stain cells in 0.1% crystal violet (in methanol) for 10 min
    0.5% crystal violet (in methanol) for 15 min
    • 00:00 1.
      Clonogenic Assay
    • 00:33 2.
      Wash with 1x PBS
    • 01:19 3.
      Fix cells (methanol: acetic acid=3:1)
    • 01:43 4.
      Incubate 5 min
    • 01:47 5.
      Remove the fixing reagent
    • 02:13 6.
      Stain cells in 0.5% crystal violet (in methanol) for 15 min
    • 02:39 7.
      wash by tap water
    • 02:51 8.
      Count the colonies (>50 cells in a colony)
    Location
    Folder name
    Clonogenic assay (Abnova版本)
    Author
    系統管理者
    Branch
    賴亮全教授
    Created
    2018-12-03 11:21:32
    Last Updated
    2018-12-26 16:08:33
    Duration
    02:58