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00:33
1.
Clonogenic Assay
00:46
2.
Wash with 1x PBS
00:23
3.
Fix cells (methanol: acetic acid=3:1)
00:04
4.
Incubate 5 min
00:26
5.
Remove the fixing reagent
00:25
6.
Stain cells in 0.5% crystal violet (in methanol) for 15 min
00:12
7.
wash by tap water
00:06
8.
Count the colonies (>50 cells in a colony)
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00:33
1.
Clonogenic Assay
00:46
2.
Wash with 1x PBS
00:23
3.
Fix cells (methanol: acetic acid=3:1)
00:04
4.
Incubate 5 min
00:26
5.
Remove the fixing reagent
00:25
6.
Stain cells in 0.5% crystal violet (in methanol) for 15 min
00:12
7.
wash by tap water
00:06
8.
Count the colonies (>50 cells in a colony)
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Print date : 2023/09/22
賴亮全實驗室
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Clonogenic assay (Abnova版本)
Clonogenic Assay
Duration: 02:58,
Browse: 3290,
Last Updated: 2018-12-26
Play Video: http://llai.cm.ntu.edu.tw/media/70
Keypoint
1.
Fix cells
methanol: acetic acid=3:1
[現行protocol] Incubate 10 min
2.
Stain cells
[現行protocol] Stain cells in 0.1% crystal violet (in methanol) for 10 min
0.5% crystal violet (in methanol) for 15 min
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00:33
1.
Clonogenic Assay
00:46
2.
Wash with 1x PBS
00:23
3.
Fix cells (methanol: acetic acid=3:1)
00:04
4.
Incubate 5 min
00:26
5.
Remove the fixing reagent
00:25
6.
Stain cells in 0.5% crystal violet (in methanol) for 15 min
00:12
7.
wash by tap water
00:06
8.
Count the colonies (>50 cells in a colony)
Location
Media Center
...
Clonogenic assay (Abnova版本)
Folder name
Clonogenic assay (Abnova版本)
Author
系統管理者
Branch
賴亮全教授
Created
2018-12-03 11:21:32
Last Updated
2018-12-26 16:08:33
Browse
3,290
Duration
02:58
Media Center
...
Clonogenic assay (Abnova版本)
1.
Clonogenic Assay