-
04:00
1.
Where is DNA located in cells?
-
01:32
2.
Vertebrate Mitochondrial Codon
-
03:17
3.
Gene Expression: Transcription
-
02:40
4.
Structure of Mature mRNA
-
01:08
5.
The Impact of Alternative RNA Splicing
-
05:23
6.
mRNA Decay
-
01:48
7.
Abnormal Translational Events Leading to Accelerated mRNA Decay
-
03:59
8.
To Explore Gene Regulation
-
01:29
9.
Loss-of-function vs. Gain-of-function
-
09:19
10.
Gene Manipulations
-
04:24
11.
Factors Influencing Transfection Efficiency
-
02:25
12.
Recombinant Bacteria
-
05:17
13.
Question: What's genomic library?
-
15:56
14.
Restriction Enzyme
-
02:02
15.
Human Genome Project
-
01:02
16.
Question:Which technique can be applied to measure gene expression?Hybridization-based techniquesPCR-based techniquesSequence-based techniquesAll of them
-
01:22
17.
Comparative Gene-expression Analysis
-
02:39
18.
Question:Which hybridization technique can be used to detect the amount of polysaccharide?Eastern blottingWestern blottingSouthern blottingNorthern blotting
-
00:35
19.
Agarose gels to resolve large fragments of DNA Polyacrylamide gelsto separate shorter nucleic acids, generally in the range of 1−1000 base pairs, based on the concentration used Gels without a denaturant (e.g., SDS): native gelsSecondary structure affects
-
00:54
20.
Blurry bands Too much DNA (100−250 ng/mm well width) Too much saltBands in the wrong place Heat nucleic acids before running on a native gelRun gel >20 V/cm ( run gel slowly sharper bands)Gel temp. >30 °CLoading buffer floats away Some salts built up i
-
00:25
21.
Question: Which gel for running microRNA samples?
-
02:10
22.
Hybridization-based techniques1
-
03:58
23.
Northern Blotting normalization
-
02:43
24.
Hybridization-based techniques2
-
00:33
25.
Microarray Platforms
-
03:08
26.
Advantages:Favor small sample sizeHigh throughput: gather data on thousands of genes (genome) in a single experimentQuantitative analysisApplication:Gene expressionGenotyping-polymorphisms (SNP) and copy number variationBinding site identification: ChIP-o
-
00:53
27.
High Throughput of Traditional Experiment