Attachment(s):
Keypoint
- 1.Transfection (e.g. 6-well plate)
1. 稀釋2ug DNA到200ul jetPRIME buffer，vortex混勻
2. 加4ul jetPRIME reagent，vortex 10秒，小烏龜離心下來
3. 室溫incubate 10分鐘
4. 每well加200ul transfection mix，搖勻
5. (可省)4h之後換成cell growth medium
6. 至少24hr之後萃RNA或protein
- 2.
- 3.
- 4.
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- Location
-
- Folder name
- Transformation / Transfection
- Author
- 石忠賢
- Branch
- 賴亮全教授
- Label
- transfection, SOP
- Created
- 2019-01-09 12:35:45
- Last Updated
- 2019-01-09 14:22:41