Open ImageJ
File à Open à Tub3.tif
Click “開啟(O)”
Image à Type à 32-bit
To further adjust image, Image à Adjust à Brightness/Contrast
Click “Auto”
Click 
Select the area to be analyzed
Analyze à Gels à Select First Lane
Drag box to 2nd lane
Repeat the procedure until all bands selected
Analyze à Gels à Plot Lanes
Click 
Isolate each peak
Click 
Click each area to get value
