• 00:00 1.
    Using ImageJ to quantify protein bands on a PAGE gel.
  • 00:35 2.
    Open an image file
  • 00:46 3.
    Convert image to grayscale
  • 01:05 4.
    Analyze gel
  • Index
  • Notes
  • Comment
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Using ImageJ to quantify protein bands on a PAGE gel.
Duration: 03:29, Browse: 918, Last Updated: 2019-08-13
Keypoint
  1. 1.

    Open ImageJ

    debfca87f95ef39a1622ebe62b95e2bf.png

    File à Open à Tub3.tif

    db3a4b1c9fa23d6dd603f26ed925efb6.png

    Click “開啟(O)”

    fb40039156c84bb0240ac5321ba8d6fd.png

    Image à Type à 32-bit

     

    263b7cdfcee1b3ef410e85fd273c58e9.png

    49e12dec1c1160efe1d9f78efc61f17d.png

    To further adjust image, Image à Adjust à Brightness/Contrast

     

     

    c09b481ec44d77d235a98e98c1c68a00.png

    a7423fbe27595f2a035e2f48ae9d08a3.png

    Click “Auto”

    ec0bb6eacc91a3c569da4b585f3dbb03.png

     

    Click 89e7b8745aa2fc0b0998e8df0d86ee48.png

    Select the area to be analyzed

    a1ddcf0691673a115442689dcf72e490.png

    Analyze à Gels à Select First Lane

     

    edca8e8a6965043d6583b767df9dad7f.png

    94b86c810c983eed34a7afd958b987da.png

    Drag box to 2nd lane

    66ae5bad19c0ae83521c7d82cd0b1bcb.png

    3b2ee04201225bb231e3b7b980e9bfad.png

    Repeat the procedure until all bands selected

    bdf65ddf5b58eb931e9363ce4518dcac.png

     

    Analyze à Gels à Plot Lanes

     

    e75a45afd92dcfb3f835048ed8f71ad7.png

    947d95e64aa350a5167e4ce0a22f69b2.png

     

     

    Click b00adcc1a097b6df69c430debd2b5247.png

    Isolate each peak

    fbd42561bc5b57b93ea4ac6d13d26069.png

     

     

    Click acfcaa986816c75c42f7ec35b4910ca2.png

    Click each area to get value

    5250c689ca5a95cc02a894853ad203d3.png

     

    • 00:00 1.
      Using ImageJ to quantify protein bands on a PAGE gel.
    • 00:35 2.
      Open an image file
    • 00:46 3.
      Convert image to grayscale
    • 01:05 4.
      Analyze gel
    Location
    Folder name
    ImageJ
    Author
    賴亮全
    Branch
    賴亮全教授
    Created
    2019-01-09 15:02:09
    Last Updated
    2019-08-13 14:18:11
    Duration
    03:29
    Reference
    2