Using ImageJ to quantify protein bands on a PAGE gel. | 賴亮全實驗室

00:00 1.
Using ImageJ to quantify protein bands on a PAGE gel.
00:35 2.
Open an image file
00:46 3.
Convert image to grayscale
01:05 4.
Analyze gel

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Using ImageJ to quantify protein bands on a PAGE gel.

Duration: 03:29, Browse: 1042, Last Updated: 2019-08-13

Keypoint

1.
Open ImageJ

File à Open à Tub3.tif

Click “開啟(O)”

Image à Type à 32-bit

To further adjust image, Image à Adjust à Brightness/Contrast

Click “Auto”

Click

Select the area to be analyzed

Analyze à Gels à Select First Lane

Drag box to 2^nd lane

Repeat the procedure until all bands selected

Analyze à Gels à Plot Lanes

Click

Isolate each peak

Click

Click each area to get value

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00:00 1.
Using ImageJ to quantify protein bands on a PAGE gel.
00:35 2.
Open an image file
00:46 3.
Convert image to grayscale
01:05 4.
Analyze gel

Location

Folder name

Author

賴亮全

Branch

賴亮全教授

Created

2019-01-09 15:02:09

Last Updated

2019-08-13 14:18:11

Duration

03:29

Reference

2