• 00:00 1.
    A quick and dirty tutorial on how to quantify a gel image using ImageJ.
  • Index
  • Notes
  • Comment
  • Fullscreen
A quick and dirty tutorial on how to quantify a gel image using ImageJ.
Duration: 05:39, Browse: 634, Last Updated: 2019-08-13
Keypoint
  1. 1.

    Open ImageJ

    7bd7b8170cfc14c09f1fb1b4a3394124.png

    File à Open à gel.jpg

    88d6daeb7704c97f98feab66508d53d3.png

    Click “開啟(O)”

    d2ba090305b8dfaeeec9dc9f250e3855.png

     

    1. Rotate gel image

    Image à Transform à Rotate

    b4b2e71969c76c29954cf58160379835.png

    Adjust angel

    Click Preview

    6c94f1053bcceaba8202c6dc0c7d55e5.png

    1.  

    Image à Lookup Tables à Invert LUT (LookUpTable)

    5370a88da3b7c386604d6848e778957d.png

     

    3c59303080e2bd434e32bc783b93bae7.png

    Click829310e814a9568c5d80dddc59cc75b5.png

    Select the area to be analyzed

    7e824fd0a0295d1483a47729c9576118.png

     

    Analyze à Gels à Gel Analyzer Options

    2d122bfd2aa7a5e3ff059915f7ed7544.png

     

    Uncheck :Invert peaks

    b424763cf9e552c822d11d25a007aaf7.png

     

    Click “OK”

    Analyze à Gels à Select First Lane (Ctrl +1)

    Analyze à Gels à Plot Lanes

    af6ec24b81c029d3827319afdc9b4e7c.png

    ccd60d6a0de6c7f81f79e80881fe4e60.png

    Click21afe06d04dcdb582467629d6a3fb3a6.png

    Isolate each peak

    35232690caed2977068207d53dc89200.png

     

    Click 334191783c78a41e359d1e45a1c56430.png

    Click each area to get value

    d5eb82351ea6dff246cb447596277dfe.png

     

    Analyze à Gels à Label Peaks

     

    dbdc155a9f6f52d984102506bb30d561.png

    7b040652344934a61f4e6bcf20c3b36f.png

    8a5fcf42d1cef7a59d01a3ce675ecd78.png

    Location
    Folder name
    ImageJ
    Author
    賴亮全
    Branch
    賴亮全教授
    Created
    2019-01-09 15:02:10
    Last Updated
    2019-08-13 14:17:04
    Duration
    05:39
    Reference
    2