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03:32
1.
凍細胞
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03:32
1.
凍細胞
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Print date : 2023/09/29
賴亮全實驗室
Media Center
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Cell Freeze
凍細胞
Duration: 03:32,
Browse: 747,
Last Updated: 2019-01-27
Play Video: http://llai.cm.ntu.edu.tw/media/495
Keypoint
要用到的試劑:PBS、medium、Trypsin、cell dye、FBS、DMSO
1.
PBS wash
,吸掉
2.
加
1 ml trypsin
放
incubator 5 min
,加
2 ml medium
,沖一沖
dish
3.
低速離心
5 min
,吸掉上清液
4.
加
1 ml medium
,數細胞,取約
10^6
個細胞
5.
800 ul
細胞
+ 100 ul FBS
加進凍細胞
tube
cell+ medium + FBS + DMSO = 1ml
6.
加
100 ul DMSO (
要避光
)
7.
Tube
放進冰盒,冰到
-80
℃
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(1)
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Q1.
DMSO的作用是什麼?
Location
Media Center
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Cell Freeze
Folder name
Cell Freeze
Author
李冠儀
Branch
賴亮全教授
Created
2019-01-26 21:22:31
Last Updated
2019-01-27 00:49:05
Browse
747
Duration
03:32
Media Center
...
Cell Freeze
1.
凍細胞
2.
凍細胞_demo
3.
Freezing Cells: Cell Culture Basics