• 00:00 1.
    凍細胞
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凍細胞
Duration: 03:32, Browse: 884, Last Updated: 2019-01-27
Keypoint
  1. 要用到的試劑:PBS、medium、Trypsin、cell dye、FBS、DMSO
  2. 1.
    PBS wash,吸掉
  3. 2.
    1 ml trypsinincubator 5 min,加2 ml medium,沖一沖dish
  4. 3.
    低速離心5 min,吸掉上清液
  5. 4.
    1 ml medium,數細胞,取約10^6個細胞
  6. 5.
    800 ul細胞+ 100 ul FBS加進凍細胞tube
  7. cell+ medium + FBS + DMSO = 1ml
  8. 6.
    100 ul DMSO (要避光)
  9. 7.
    Tube放進冰盒,冰到 -80
    Location
    Folder name
    Cell Freeze
    Author
    李冠儀
    Branch
    賴亮全教授
    Created
    2019-01-26 21:22:31
    Last Updated
    2019-01-27 00:49:05
    Duration
    03:32