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00:59
1.
index 1
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00:27
2.
Slide 2
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01:35
3.
Introduction
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01:17
4.
Slide 4
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00:39
5.
Examination of universally expressed circRNAs
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02:11
6.
Treated HeLa cells with 8 stressors followed by the examination of universally expressed circRNAs
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01:01
7.
circRNAs are degraded upon Poly I:C treatment
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00:00
8.
Global reduction of high confidence circRNAs upon poly(I:C) for 6 h (C) or EMCV for 24 h
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00:59
9.
circRNAs are degraded upon Poly I:C treatment
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01:13
10.
Treated HeLa cells with 8 stressors followed by the examination of universally expressed circRNAs
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01:00
11.
circRNAs are degraded upon Poly I:C treatment
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02:17
12.
Treated HeLa cells with 8 stressors followed by the examination of universally expressed circRNAs
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00:01
13.
circRNAs are degraded upon Poly I:C treatment
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00:08
14.
Global reduction of high confidence circRNAs upon poly(I:C) for 6 h (C) or EMCV for 24 h
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00:00
15.
circRNAs are degraded upon Poly I:C treatment
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04:06
16.
Global reduction of high confidence circRNAs upon poly(I:C) for 6 h (C) or EMCV for 24 h
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00:00
17.
circRNAs are degraded upon Poly I:C treatment
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00:00
18.
Treated HeLa cells with 8 stressors followed by the examination of universally expressed circRNAs
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00:01
19.
circRNAs are degraded upon Poly I:C treatment
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00:07
20.
Global reduction of high confidence circRNAs upon poly(I:C) for 6 h (C) or EMCV for 24 h
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00:14
21.
Slide 9
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03:19
22.
RNase L “may” be responsible for circRNA degradation upon poly(I:C) and viral stimulations
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00:01
23.
Slide 9
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00:27
24.
RNase L “may” be responsible for circRNA degradation upon poly(I:C) and viral stimulations
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00:02
25.
Slide 11
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00:05
26.
RNase L “may” be responsible for circRNA degradation upon poly(I:C) and viral stimulations
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02:01
27.
Slide 11
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01:39
28.
RNase L is responsible for global circRNA degradation in cells treated with poly(I:C)
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00:33
29.
The endonuclease activity of RNase L is requiredfor circRNA degradation
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00:00
30.
Slide 14
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01:42
31.
The endonuclease activity of RNase L is requiredfor circRNA degradation
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00:00
32.
Slide 14
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00:13
33.
The endonuclease activity of RNase L is requiredfor circRNA degradation
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00:05
34.
Slide 14
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00:01
35.
The endonuclease activity of RNase L is requiredfor circRNA degradation
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00:11
36.
Slide 14
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00:10
37.
Slide 15
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00:02
38.
In vitro binding and competition screening assays with purified immune factors
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00:22
39.
Slide 15
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01:22
40.
In vitro binding and competition screening assays with purified immune factors
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00:04
41.
Slide 15
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00:48
42.
In vitro binding and competition screening assays with purified immune factors
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05:37
43.
circRNAs prefer to bind to nucleic acid receptors with antiviral activity
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00:06
44.
circRNAs prefer to bind to nucleic acid receptors with antiviral activity
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00:00
45.
circRNAs prefer to bind to nucleic acid receptors with antiviral activity
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00:01
46.
circRNAs prefer to bind to nucleic acid receptors with antiviral activity
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00:00
47.
dsRBMs of PKR are required for binding to circPOLR2A
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00:29
48.
circRNAs prefer to bind to nucleic acid receptors with antiviral activity
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01:48
49.
dsRBMs of PKR are required for binding to circPOLR2A
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00:00
50.
circRNAs prefer to bind to nucleic acid receptors with antiviral activity
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00:00
51.
circRNAs prefer to bind to nucleic acid receptors with antiviral activity
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00:33
52.
circRNAs prefer to bind to nucleic acid receptors with antiviral activity
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00:01
53.
dsRBMs of PKR are required for binding to circPOLR2A
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00:19
54.
circRNAs prefer to bind to nucleic acid receptors with antiviral activity
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00:00
55.
dsRBMs of PKR are required for binding to circPOLR2A
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00:41
56.
PKR is an IFN-inducible Ser/Thr protein kinase directly activated by dsRNA
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02:04
57.
Slide 21
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00:11
58.
Slide 22
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00:00
59.
Slide 23
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00:01
60.
Slide 22
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00:25
61.
Slide 23
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01:02
62.
Slide 24
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00:02
63.
Comparison of paired circular and linear RNA structures by SHAPE-MaP
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00:24
64.
Slide 24
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01:12
65.
Comparison of paired circular and linear RNA structures by SHAPE-MaP
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01:13
66.
Linear POLR2A is relatively unstructured within circPOLR2A-forming region
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00:31
67.
circRNAs have distinct structural conformation from their linear cognate RNAs
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00:32
68.
Many examined circRNAs tend to form imperfect duplex RNA regions
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00:03
69.
Slide 29
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01:12
70.
Slide 30
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00:28
71.
Slide 31
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00:08
72.
Slide 32
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00:20
73.
Slide 33
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01:20
74.
Most examined circRNAs could form 1–4 dsRNA regions.Hypothesis : Per cell may contains > 10,000~20,000 intra-dsRNA regions and that such circRNAs represent a type of endogenous inhibitors for PKR.
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00:38
75.
Overexpression of circRNAs in Hela cell
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00:04
76.
Most examined circRNAs could form 1–4 dsRNA regions.Hypothesis : Per cell may contains > 10,000~20,000 intra-dsRNA regions and that such circRNAs represent a type of endogenous inhibitors for PKR.
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00:28
77.
Overexpression of circRNAs in Hela cell
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00:58
78.
Overexpression of circPOLR2A in HeLa cells inhibits PKR phosphorylation upon poly(I:C) stimulation
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01:25
79.
Overexpression of the non-dsRNA-containing circRNA in HeLa cells does not suppress PKR phosphorylation upon poly(I:C) stimulation.
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00:22
80.
Slide 38
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00:01
81.
Slide 39
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00:00
82.
Reduced PKR activation in RNase L KO HeLa cells stimulated with poly(I:C)
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00:25
83.
Slide 39
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00:41
84.
Reduced PKR activation in RNase L KO HeLa cells stimulated with poly(I:C)
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00:22
85.
Overexpression of circPOLR2A in HeLa cells inhibitsPKR phosphorylation upon EMCV infection
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00:07
86.
Slide 42
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01:28
87.
Dysregulated circRNA expression and PKR activation in SLE
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00:28
88.
circRNA reduction in PBMCs derived from SLE patients
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01:45
89.
Slide 45
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00:37
90.
Global reduction of circRNA expression in SLE patients (RNA-seq)
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00:29
91.
Slide 47
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01:02
92.
circPOLR2A overexpression attenuates aberrant PKR and EIF2α phosphorylation in PBMCs isolated from three SLE patients
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00:00
93.
circPOLR2A overexpression attenuates aberrant PKR and EIF2α phosphorylation in T cells isolated from three SLE patients
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00:15
94.
circPOLR2A overexpression attenuates aberrant PKR and EIF2α phosphorylation in PBMCs isolated from three SLE patients
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00:04
95.
circPOLR2A overexpression attenuates aberrant PKR and EIF2α phosphorylation in T cells isolated from three SLE patients
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00:03
96.
circPOLR2A overexpression attenuates aberrant PKR and EIF2α phosphorylation in PBMCs isolated from three SLE patients
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00:07
97.
circPOLR2A overexpression attenuates aberrant PKR and EIF2α phosphorylation in T cells isolated from three SLE patients
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00:12
98.
Conclusion
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00:51
99.
Slide 51
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00:30
100.
Highlights
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00:51
101.
circPOLR2A overexpression attenuates aberrant PKR and EIF2α phosphorylation in PBMCs isolated from three SLE patients