-
00:21
1.
index 1
-
00:13
2.
Engineering circular RNA for potent and stable translation in eukaryotic cellsR. Alexander Wesselhoeft, Piotr S. Kowalski & Daniel G. Anderson Nature Communications 9, Article number: 2629 (2018)
-
00:51
3.
Backsplicing of circRNA
-
00:29
4.
CircRNA are more resistant to nucleases and stable
-
00:09
5.
Aim
-
00:02
6.
Self-Splicing Introns
-
00:00
7.
Aim
-
00:00
8.
CircRNA are more resistant to nucleases and stable
-
01:42
9.
Backsplicing of circRNA
-
01:20
10.
Self-Splicing Introns
-
01:10
11.
IRES (Internal Ribosomal Entry Site)
-
00:01
12.
Results
-
00:50
13.
IRES (Internal Ribosomal Entry Site)
-
00:02
14.
Results
-
01:10
15.
Premuted intron-exon(PIE) splicing
-
00:02
16.
Addition of homology arms
-
00:08
17.
Premuted intron-exon(PIE) splicing
-
00:05
18.
Addition of homology arms
-
00:59
19.
Premuted intron-exon(PIE) splicing
-
00:30
20.
Addition of homology arms
-
00:05
21.
Premuted intron-exon(PIE) splicing
-
01:52
22.
Addition of homology arms
-
00:10
23.
Premuted intron-exon(PIE) splicing
-
04:56
24.
Addition of homology arms
-
00:44
25.
The effect of spacers on splicing
-
00:01
26.
Addition of homology arms
-
01:14
27.
Premuted intron-exon(PIE) splicing
-
00:00
28.
Addition of homology arms
-
00:42
29.
The effect of spacers on splicing
-
01:37
30.
The effect of switching to the Anabaena catalytic intron
-
03:00
31.
Evaluation of circularization efficacy and translation from de-novo engineered precursor RNA.
-
00:59
32.
Evaluation of circularization efficacy and translation from de-novo engineered precursor RNA.
-
00:04
33.
IRES efficacy in varying sequence contexts
-
00:17
34.
Evaluation of circularization efficacy and translation from de-novo engineered precursor RNA.
-
02:13
35.
IRES efficacy in varying sequence contexts
-
00:55
36.
IRES efficacy in varying cell
-
00:02
37.
IRES efficacy in varying sequence contexts
-
00:06
38.
IRES efficacy in varying cell
-
03:01
39.
HPLC purification of circRNA from splicing reactions
-
00:02
40.
HPLC purification of circRNA from splicing reactions
-
01:13
41.
HPLC purification of circRNA from splicing reactions
-
00:52
42.
HPLC purification of circRNA from splicing reactions
-
00:01
43.
Translation efficacy of circRNA compared to linear mRNA
-
00:42
44.
HPLC purification of circRNA from splicing reactions
-
05:26
45.
Translation efficacy of circRNA compared to linear mRNA
-
00:10
46.
Translation efficacy of circRNA compared to linear mRNA
-
01:58
47.
Slide 20