• 00:21 1.
    index 1
  • 00:13 2.
    Engineering circular RNA for potent and stable translation in eukaryotic cellsR. Alexander Wesselhoeft, Piotr S. Kowalski & Daniel G. Anderson Nature Communications 9, Article number: 2629 (2018)
  • 00:51 3.
    Backsplicing of circRNA
  • 00:29 4.
    CircRNA are more resistant to nucleases and stable
  • 00:09 5.
    Aim
  • 00:02 6.
    Self-Splicing Introns
  • 00:00 7.
    Aim
  • 00:00 8.
    CircRNA are more resistant to nucleases and stable
  • 01:42 9.
    Backsplicing of circRNA
  • 01:20 10.
    Self-Splicing Introns
  • 01:10 11.
    IRES (Internal Ribosomal Entry Site)
  • 00:01 12.
    Results
  • 00:50 13.
    IRES (Internal Ribosomal Entry Site)
  • 00:02 14.
    Results
  • 01:10 15.
    Premuted intron-exon(PIE) splicing
  • 00:02 16.
    Addition of homology arms
  • 00:08 17.
    Premuted intron-exon(PIE) splicing
  • 00:05 18.
    Addition of homology arms
  • 00:59 19.
    Premuted intron-exon(PIE) splicing
  • 00:30 20.
    Addition of homology arms
  • 00:05 21.
    Premuted intron-exon(PIE) splicing
  • 01:52 22.
    Addition of homology arms
  • 00:10 23.
    Premuted intron-exon(PIE) splicing
  • 04:56 24.
    Addition of homology arms
  • 00:44 25.
    The effect of spacers on splicing
  • 00:01 26.
    Addition of homology arms
  • 01:14 27.
    Premuted intron-exon(PIE) splicing
  • 00:00 28.
    Addition of homology arms
  • 00:42 29.
    The effect of spacers on splicing
  • 01:37 30.
    The effect of switching to the Anabaena catalytic intron
  • 03:00 31.
    Evaluation of circularization efficacy and translation from de-novo engineered precursor RNA.
  • 00:59 32.
    Evaluation of circularization efficacy and translation from de-novo engineered precursor RNA.
  • 00:04 33.
    IRES efficacy in varying sequence contexts
  • 00:17 34.
    Evaluation of circularization efficacy and translation from de-novo engineered precursor RNA.
  • 02:13 35.
    IRES efficacy in varying sequence contexts
  • 00:55 36.
    IRES efficacy in varying cell
  • 00:02 37.
    IRES efficacy in varying sequence contexts
  • 00:06 38.
    IRES efficacy in varying cell
  • 03:01 39.
    HPLC purification of circRNA from splicing reactions
  • 00:02 40.
    HPLC purification of circRNA from splicing reactions
  • 01:13 41.
    HPLC purification of circRNA from splicing reactions
  • 00:52 42.
    HPLC purification of circRNA from splicing reactions
  • 00:01 43.
    Translation efficacy of circRNA compared to linear mRNA
  • 00:42 44.
    HPLC purification of circRNA from splicing reactions
  • 05:26 45.
    Translation efficacy of circRNA compared to linear mRNA
  • 00:10 46.
    Translation efficacy of circRNA compared to linear mRNA
  • 01:58 47.
    Slide 20
  • Index
  • Notes
  • Comment
  • Fullscreen
20190603-lab meeting_冠儀
Duration: 42:51, Browse: 520, Last Updated: 2019-06-13
Attachment(s):
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    • 00:21 1.
      index 1
    • 00:13 2.
      Engineering circular RNA for potent and stable translation in eukaryotic cellsR. Alexander Wesselhoeft, Piotr S. Kowalski & Daniel G. Anderson Nature Communications 9, Article number: 2629 (2018)
    • 00:51 3.
      Backsplicing of circRNA
    • 00:29 4.
      CircRNA are more resistant to nucleases and stable
    • 00:09 5.
      Aim
    • 00:02 6.
      Self-Splicing Introns
    • 00:00 7.
      Aim
    • 00:00 8.
      CircRNA are more resistant to nucleases and stable
    • 01:42 9.
      Backsplicing of circRNA
    • 01:20 10.
      Self-Splicing Introns
    • 01:10 11.
      IRES (Internal Ribosomal Entry Site)
    • 00:01 12.
      Results
    • 00:50 13.
      IRES (Internal Ribosomal Entry Site)
    • 00:02 14.
      Results
    • 01:10 15.
      Premuted intron-exon(PIE) splicing
    • 00:02 16.
      Addition of homology arms
    • 00:08 17.
      Premuted intron-exon(PIE) splicing
    • 00:05 18.
      Addition of homology arms
    • 00:59 19.
      Premuted intron-exon(PIE) splicing
    • 00:30 20.
      Addition of homology arms
    • 00:05 21.
      Premuted intron-exon(PIE) splicing
    • 01:52 22.
      Addition of homology arms
    • 00:10 23.
      Premuted intron-exon(PIE) splicing
    • 04:56 24.
      Addition of homology arms
    • 00:44 25.
      The effect of spacers on splicing
    • 00:01 26.
      Addition of homology arms
    • 01:14 27.
      Premuted intron-exon(PIE) splicing
    • 00:00 28.
      Addition of homology arms
    • 00:42 29.
      The effect of spacers on splicing
    • 01:37 30.
      The effect of switching to the Anabaena catalytic intron
    • 03:00 31.
      Evaluation of circularization efficacy and translation from de-novo engineered precursor RNA.
    • 00:59 32.
      Evaluation of circularization efficacy and translation from de-novo engineered precursor RNA.
    • 00:04 33.
      IRES efficacy in varying sequence contexts
    • 00:17 34.
      Evaluation of circularization efficacy and translation from de-novo engineered precursor RNA.
    • 02:13 35.
      IRES efficacy in varying sequence contexts
    • 00:55 36.
      IRES efficacy in varying cell
    • 00:02 37.
      IRES efficacy in varying sequence contexts
    • 00:06 38.
      IRES efficacy in varying cell
    • 03:01 39.
      HPLC purification of circRNA from splicing reactions
    • 00:02 40.
      HPLC purification of circRNA from splicing reactions
    • 01:13 41.
      HPLC purification of circRNA from splicing reactions
    • 00:52 42.
      HPLC purification of circRNA from splicing reactions
    • 00:01 43.
      Translation efficacy of circRNA compared to linear mRNA
    • 00:42 44.
      HPLC purification of circRNA from splicing reactions
    • 05:26 45.
      Translation efficacy of circRNA compared to linear mRNA
    • 00:10 46.
      Translation efficacy of circRNA compared to linear mRNA
    • 01:58 47.
      Slide 20
      1. Q1.
        What did authors do to increase the efficiency of circularization and protein translation?
      Location
      1. Media Center
      2. ...
      3. 2019
      Folder name
      2019
      Author
      鄭捷登
      Branch
      賴亮全教授
      Created
      2019-06-03 17:02:52
      Last Updated
      2019-06-13 12:33:54
      Browse
      520
      Duration
      42:51
      1. Media Center
      2. ...
      3. 2019