• 00:00 1.
    index 1
  • 00:21 2.
    Engineering circular RNA for potent and stable translation in eukaryotic cellsR. Alexander Wesselhoeft, Piotr S. Kowalski & Daniel G. Anderson Nature Communications 9, Article number: 2629 (2018)
  • 00:34 3.
    Backsplicing of circRNA
  • 01:26 4.
    CircRNA are more resistant to nucleases and stable
  • 01:55 5.
    Aim
  • 02:05 6.
    Self-Splicing Introns
  • 02:08 7.
    Aim
  • 02:08 8.
    CircRNA are more resistant to nucleases and stable
  • 02:09 9.
    Backsplicing of circRNA
  • 03:51 10.
    Self-Splicing Introns
  • 05:12 11.
    IRES (Internal Ribosomal Entry Site)
  • 06:23 12.
    Results
  • 06:24 13.
    IRES (Internal Ribosomal Entry Site)
  • 07:15 14.
    Results
  • 07:18 15.
    Premuted intron-exon(PIE) splicing
  • 08:29 16.
    Addition of homology arms
  • 08:32 17.
    Premuted intron-exon(PIE) splicing
  • 08:40 18.
    Addition of homology arms
  • 08:45 19.
    Premuted intron-exon(PIE) splicing
  • 09:45 20.
    Addition of homology arms
  • 10:15 21.
    Premuted intron-exon(PIE) splicing
  • 10:20 22.
    Addition of homology arms
  • 12:13 23.
    Premuted intron-exon(PIE) splicing
  • 12:23 24.
    Addition of homology arms
  • 17:20 25.
    The effect of spacers on splicing
  • 18:04 26.
    Addition of homology arms
  • 18:06 27.
    Premuted intron-exon(PIE) splicing
  • 19:21 28.
    Addition of homology arms
  • 19:21 29.
    The effect of spacers on splicing
  • 20:03 30.
    The effect of switching to the Anabaena catalytic intron
  • 21:41 31.
    Evaluation of circularization efficacy and translation from de-novo engineered precursor RNA.
  • 24:41 32.
    Evaluation of circularization efficacy and translation from de-novo engineered precursor RNA.
  • 25:40 33.
    IRES efficacy in varying sequence contexts
  • 25:45 34.
    Evaluation of circularization efficacy and translation from de-novo engineered precursor RNA.
  • 26:02 35.
    IRES efficacy in varying sequence contexts
  • 28:15 36.
    IRES efficacy in varying cell
  • 29:11 37.
    IRES efficacy in varying sequence contexts
  • 29:14 38.
    IRES efficacy in varying cell
  • 29:20 39.
    HPLC purification of circRNA from splicing reactions
  • 32:22 40.
    HPLC purification of circRNA from splicing reactions
  • 32:25 41.
    HPLC purification of circRNA from splicing reactions
  • 33:38 42.
    HPLC purification of circRNA from splicing reactions
  • 34:31 43.
    Translation efficacy of circRNA compared to linear mRNA
  • 34:33 44.
    HPLC purification of circRNA from splicing reactions
  • 35:15 45.
    Translation efficacy of circRNA compared to linear mRNA
  • 40:42 46.
    Translation efficacy of circRNA compared to linear mRNA
  • 40:52 47.
    Slide 20
  • Index
  • Notes
  • Comment
  • Fullscreen
20190603-lab meeting_冠儀
Duration: 42:51, Browse: 768, Last Updated: 2019-06-13
    • 00:00 1.
      index 1
    • 00:21 2.
      Engineering circular RNA for potent and stable translation in eukaryotic cellsR. Alexander Wesselhoeft, Piotr S. Kowalski & Daniel G. Anderson Nature Communications 9, Article number: 2629 (2018)
    • 00:34 3.
      Backsplicing of circRNA
    • 01:26 4.
      CircRNA are more resistant to nucleases and stable
    • 01:55 5.
      Aim
    • 02:05 6.
      Self-Splicing Introns
    • 02:08 7.
      Aim
    • 02:08 8.
      CircRNA are more resistant to nucleases and stable
    • 02:09 9.
      Backsplicing of circRNA
    • 03:51 10.
      Self-Splicing Introns
    • 05:12 11.
      IRES (Internal Ribosomal Entry Site)
    • 06:23 12.
      Results
    • 06:24 13.
      IRES (Internal Ribosomal Entry Site)
    • 07:15 14.
      Results
    • 07:18 15.
      Premuted intron-exon(PIE) splicing
    • 08:29 16.
      Addition of homology arms
    • 08:32 17.
      Premuted intron-exon(PIE) splicing
    • 08:40 18.
      Addition of homology arms
    • 08:45 19.
      Premuted intron-exon(PIE) splicing
    • 09:45 20.
      Addition of homology arms
    • 10:15 21.
      Premuted intron-exon(PIE) splicing
    • 10:20 22.
      Addition of homology arms
    • 12:13 23.
      Premuted intron-exon(PIE) splicing
    • 12:23 24.
      Addition of homology arms
    • 17:20 25.
      The effect of spacers on splicing
    • 18:04 26.
      Addition of homology arms
    • 18:06 27.
      Premuted intron-exon(PIE) splicing
    • 19:21 28.
      Addition of homology arms
    • 19:21 29.
      The effect of spacers on splicing
    • 20:03 30.
      The effect of switching to the Anabaena catalytic intron
    • 21:41 31.
      Evaluation of circularization efficacy and translation from de-novo engineered precursor RNA.
    • 24:41 32.
      Evaluation of circularization efficacy and translation from de-novo engineered precursor RNA.
    • 25:40 33.
      IRES efficacy in varying sequence contexts
    • 25:45 34.
      Evaluation of circularization efficacy and translation from de-novo engineered precursor RNA.
    • 26:02 35.
      IRES efficacy in varying sequence contexts
    • 28:15 36.
      IRES efficacy in varying cell
    • 29:11 37.
      IRES efficacy in varying sequence contexts
    • 29:14 38.
      IRES efficacy in varying cell
    • 29:20 39.
      HPLC purification of circRNA from splicing reactions
    • 32:22 40.
      HPLC purification of circRNA from splicing reactions
    • 32:25 41.
      HPLC purification of circRNA from splicing reactions
    • 33:38 42.
      HPLC purification of circRNA from splicing reactions
    • 34:31 43.
      Translation efficacy of circRNA compared to linear mRNA
    • 34:33 44.
      HPLC purification of circRNA from splicing reactions
    • 35:15 45.
      Translation efficacy of circRNA compared to linear mRNA
    • 40:42 46.
      Translation efficacy of circRNA compared to linear mRNA
    • 40:52 47.
      Slide 20
    Location
    Folder name
    2019
    Author
    鄭捷登
    Branch
    賴亮全教授
    Created
    2019-06-03 17:02:52
    Last Updated
    2019-06-13 12:33:54
    Duration
    42:51