• 00:00 1.
    index 1
  • 00:21 2.
    Engineering circular RNA for potent and stable translation in eukaryotic cellsR. Alexander Wesselhoeft, Piotr S. Kowalski & Daniel G. Anderson Nature Communications 9, Article number: 2629 (2018)
  • 00:34 3.
    Backsplicing of circRNA
  • 01:26 4.
    CircRNA are more resistant to nucleases and stable
  • 01:55 5.
    Aim
  • 02:05 6.
    Self-Splicing Introns
  • 02:08 7.
    Aim
  • 02:08 8.
    CircRNA are more resistant to nucleases and stable
  • 02:09 9.
    Backsplicing of circRNA
  • 03:51 10.
    Self-Splicing Introns
  • 05:12 11.
    IRES (Internal Ribosomal Entry Site)
  • 06:23 12.
    Results
  • 06:24 13.
    IRES (Internal Ribosomal Entry Site)
  • 07:15 14.
    Results
  • 07:18 15.
    Premuted intron-exon(PIE) splicing
  • 08:29 16.
    Addition of homology arms
  • 08:32 17.
    Premuted intron-exon(PIE) splicing
  • 08:40 18.
    Addition of homology arms
  • 08:45 19.
    Premuted intron-exon(PIE) splicing
  • 09:45 20.
    Addition of homology arms
  • 10:15 21.
    Premuted intron-exon(PIE) splicing
  • 10:20 22.
    Addition of homology arms
  • 12:13 23.
    Premuted intron-exon(PIE) splicing
  • 12:23 24.
    Addition of homology arms
  • 17:20 25.
    The effect of spacers on splicing
  • 18:04 26.
    Addition of homology arms
  • 18:06 27.
    Premuted intron-exon(PIE) splicing
  • 19:21 28.
    Addition of homology arms
  • 19:21 29.
    The effect of spacers on splicing
  • 20:03 30.
    The effect of switching to the Anabaena catalytic intron
  • 21:41 31.
    Evaluation of circularization efficacy and translation from de-novo engineered precursor RNA.
  • 24:41 32.
    Evaluation of circularization efficacy and translation from de-novo engineered precursor RNA.
  • 25:40 33.
    IRES efficacy in varying sequence contexts
  • 25:45 34.
    Evaluation of circularization efficacy and translation from de-novo engineered precursor RNA.
  • 26:02 35.
    IRES efficacy in varying sequence contexts
  • 28:15 36.
    IRES efficacy in varying cell
  • 29:11 37.
    IRES efficacy in varying sequence contexts
  • 29:14 38.
    IRES efficacy in varying cell
  • 29:20 39.
    HPLC purification of circRNA from splicing reactions
  • 32:22 40.
    HPLC purification of circRNA from splicing reactions
  • 32:25 41.
    HPLC purification of circRNA from splicing reactions
  • 33:38 42.
    HPLC purification of circRNA from splicing reactions
  • 34:31 43.
    Translation efficacy of circRNA compared to linear mRNA
  • 34:33 44.
    HPLC purification of circRNA from splicing reactions
  • 35:15 45.
    Translation efficacy of circRNA compared to linear mRNA
  • 40:42 46.
    Translation efficacy of circRNA compared to linear mRNA
  • 40:52 47.
    Slide 20
  • 索引
  • 筆記
  • 討論
  • 全螢幕
20190603-lab meeting_冠儀
長度: 42:51, 瀏覽: 706, 最近修訂: 2019-06-13
  • 00:00 1.
    index 1
  • 00:21 2.
    Engineering circular RNA for potent and stable translation in eukaryotic cellsR. Alexander Wesselhoeft, Piotr S. Kowalski & Daniel G. Anderson Nature Communications 9, Article number: 2629 (2018)
  • 00:34 3.
    Backsplicing of circRNA
  • 01:26 4.
    CircRNA are more resistant to nucleases and stable
  • 01:55 5.
    Aim
  • 02:05 6.
    Self-Splicing Introns
  • 02:08 7.
    Aim
  • 02:08 8.
    CircRNA are more resistant to nucleases and stable
  • 02:09 9.
    Backsplicing of circRNA
  • 03:51 10.
    Self-Splicing Introns
  • 05:12 11.
    IRES (Internal Ribosomal Entry Site)
  • 06:23 12.
    Results
  • 06:24 13.
    IRES (Internal Ribosomal Entry Site)
  • 07:15 14.
    Results
  • 07:18 15.
    Premuted intron-exon(PIE) splicing
  • 08:29 16.
    Addition of homology arms
  • 08:32 17.
    Premuted intron-exon(PIE) splicing
  • 08:40 18.
    Addition of homology arms
  • 08:45 19.
    Premuted intron-exon(PIE) splicing
  • 09:45 20.
    Addition of homology arms
  • 10:15 21.
    Premuted intron-exon(PIE) splicing
  • 10:20 22.
    Addition of homology arms
  • 12:13 23.
    Premuted intron-exon(PIE) splicing
  • 12:23 24.
    Addition of homology arms
  • 17:20 25.
    The effect of spacers on splicing
  • 18:04 26.
    Addition of homology arms
  • 18:06 27.
    Premuted intron-exon(PIE) splicing
  • 19:21 28.
    Addition of homology arms
  • 19:21 29.
    The effect of spacers on splicing
  • 20:03 30.
    The effect of switching to the Anabaena catalytic intron
  • 21:41 31.
    Evaluation of circularization efficacy and translation from de-novo engineered precursor RNA.
  • 24:41 32.
    Evaluation of circularization efficacy and translation from de-novo engineered precursor RNA.
  • 25:40 33.
    IRES efficacy in varying sequence contexts
  • 25:45 34.
    Evaluation of circularization efficacy and translation from de-novo engineered precursor RNA.
  • 26:02 35.
    IRES efficacy in varying sequence contexts
  • 28:15 36.
    IRES efficacy in varying cell
  • 29:11 37.
    IRES efficacy in varying sequence contexts
  • 29:14 38.
    IRES efficacy in varying cell
  • 29:20 39.
    HPLC purification of circRNA from splicing reactions
  • 32:22 40.
    HPLC purification of circRNA from splicing reactions
  • 32:25 41.
    HPLC purification of circRNA from splicing reactions
  • 33:38 42.
    HPLC purification of circRNA from splicing reactions
  • 34:31 43.
    Translation efficacy of circRNA compared to linear mRNA
  • 34:33 44.
    HPLC purification of circRNA from splicing reactions
  • 35:15 45.
    Translation efficacy of circRNA compared to linear mRNA
  • 40:42 46.
    Translation efficacy of circRNA compared to linear mRNA
  • 40:52 47.
    Slide 20
位置
資料夾名稱
2019
發表人
鄭捷登
單位
賴亮全教授
建立
2019-06-03 17:02:52
最近修訂
2019-06-13 12:33:54
長度
42:51