20190701-lab meeting_品豪 | 賴亮全實驗室

索引

00:00 1.
index 1
00:21 2.
 Introduction
00:56 3.
 Introduction of SEMA5A
00:58 4.
 Introduction
01:01 5.
 Introduction of SEMA5A
02:51 6.
 Down-regulation of SEMA5A in lung adenocarcinoma tissues and cell lines
04:22 7.
 SEMA5A-regulated genes were involved in cell growth and proliferation
05:48 8.
 5A suppressed proliferation in lung cancer cells.
05:58 9.
 SEMA5A-regulated genes were involved in cell growth and proliferation
06:03 10.
 5A suppressed proliferation in lung cancer cells.
07:47 11.
 Lower expression and hypermethylation of 5A in lung cancer cells.
09:08 12.
 Summary of chapter 2
09:37 13.
銅葉綠素…
13:38 14.
 Introduction of HPLC-PCA
14:57 15.
Slide 11
15:41 16.
 Chapter 4: Genomic approaches on Chinese medicine concept--- “qi”
18:31 17.
 Internal transcribed spacer (ITS)
18:32 18.
 Chapter 4: Genomic approaches on Chinese medicine concept--- “qi”
18:34 19.
 Internal transcribed spacer (ITS)
19:28 20.
 Viability test of crudes on cancerous cells
19:31 21.
 Internal transcribed spacer (ITS)
19:35 22.
 Viability test of crudes on cancerous cells
20:02 23.
Slide 15
20:04 24.
 Viability test of crudes on cancerous cells
20:06 25.
Slide 15
20:23 26.
 Markers identification
21:21 27.
 Markers identification
21:46 28.
 Summary of chapter 3 and 4
22:09 29.
 Chapter : Genomic approaches on a newly synthesized small molecule compound, MCDC
24:13 30.
Slide 20
25:13 31.
 Synthesis scheme of 1-substituted carbazolyl-1,2,3,4-tetrahydro-β-carboline and carbazolyl-3,4-dihydro-β-carboline derivatives
25:29 32.
Slide 22
25:59 33.
Slide 23
28:25 34.
 IC50 of MCDC in A549 and MCF7 cells
29:04 35.
 MIF-MCDC virtual screen-idTarget
30:27 36.
 MIF-MCDC virtual screen-Discovery studio
30:55 37.
 MIF-MCDC in vitro-Hydrogen-deuterium exchange
32:50 38.
 MIF-MCDC in cell line model.
33:19 39.
 MIF-MCDC in cell line model.
33:20 40.
 MIF-MCDC in cell line model.
34:03 41.
 MIF-MCDC in cell line model.
35:30 42.
 MCDC inhibits cell cycle-related protein expression.
36:28 43.
 MCDC inhibits cell cycle-related protein expression.
37:01 44.
 Summary of chapter 5
37:22 45.
 Manual adjustment of HPLC readings
37:26 46.
 Samples not applicable to ITS analysis
37:27 47.
Slide 35
37:27 48.
 Structures not provided in idTarget
37:28 49.
 Hydrophobicity of MCDC interfered the results of HDX and Biacore.
37:31 50.
 Endogenous MIF expression interfered U251 viability
37:31 51.
Slide 39
37:31 52.
Slide 40
37:32 53.
Slide 39
37:40 54.
 Endogenous MIF expression interfered U251 viability
37:41 55.
 Hydrophobicity of MCDC interfered the results of HDX and Biacore.
37:41 56.
 Structures not provided in idTarget
37:42 57.
Slide 35
37:42 58.
 Samples not applicable to ITS analysis
37:43 59.
 Manual adjustment of HPLC readings
37:43 60.
 Summary of chapter 5
37:43 61.
 MCDC inhibits cell cycle-related protein expression.
37:44 62.
 MCDC inhibits cell cycle-related protein expression.
37:44 63.
 MIF-MCDC in cell line model.
37:45 64.
 MIF-MCDC in cell line model.
37:45 65.
 MIF-MCDC in vitro-Hydrogen-deuterium exchange
37:46 66.
 MIF-MCDC virtual screen-Discovery studio
37:46 67.
 MIF-MCDC virtual screen-idTarget
37:46 68.
 IC50 of MCDC in A549 and MCF7 cells
37:47 69.
Slide 23
37:48 70.
Slide 22
37:48 71.
 Synthesis scheme of 1-substituted carbazolyl-1,2,3,4-tetrahydro-β-carboline and carbazolyl-3,4-dihydro-β-carboline derivatives
37:48 72.
Slide 20
37:49 73.
 Chapter : Genomic approaches on a newly synthesized small molecule compound, MCDC
37:49 74.
 Summary of chapter 3 and 4
37:50 75.
 Markers identification
37:50 76.
 Markers identification
37:50 77.
Slide 15
37:51 78.
 Viability test of crudes on cancerous cells
37:51 79.
 Internal transcribed spacer (ITS)
37:52 80.
 Chapter 4: Genomic approaches on Chinese medicine concept--- “qi”
37:52 81.
Slide 11
37:53 82.
 Introduction of HPLC-PCA
37:53 83.
銅葉綠素…
37:54 84.
 Summary of chapter 2
37:55 85.
 Lower expression and hypermethylation of 5A in lung cancer cells.
37:57 86.
 5A suppressed proliferation in lung cancer cells.
37:57 87.
 SEMA5A-regulated genes were involved in cell growth and proliferation
37:58 88.
 Down-regulation of SEMA5A in lung adenocarcinoma tissues and cell lines
37:58 89.
 Introduction of SEMA5A
37:59 90.
 Introduction
38:28 91.
 Introduction of SEMA5A
38:31 92.
 Down-regulation of SEMA5A in lung adenocarcinoma tissues and cell lines
38:33 93.
 SEMA5A-regulated genes were involved in cell growth and proliferation
38:37 94.
 5A suppressed proliferation in lung cancer cells.
38:48 95.
 SEMA5A-regulated genes were involved in cell growth and proliferation
38:49 96.
 Down-regulation of SEMA5A in lung adenocarcinoma tissues and cell lines
39:24 97.
 SEMA5A-regulated genes were involved in cell growth and proliferation
39:25 98.
 5A suppressed proliferation in lung cancer cells.
39:25 99.
 Lower expression and hypermethylation of 5A in lung cancer cells.
39:26 100.
 5A suppressed proliferation in lung cancer cells.
40:01 101.
 SEMA5A-regulated genes were involved in cell growth and proliferation
40:01 102.
 Down-regulation of SEMA5A in lung adenocarcinoma tissues and cell lines
40:02 103.
 Introduction of SEMA5A
40:03 104.
 Down-regulation of SEMA5A in lung adenocarcinoma tissues and cell lines
40:43 105.
 SEMA5A-regulated genes were involved in cell growth and proliferation
40:43 106.
 5A suppressed proliferation in lung cancer cells.
42:18 107.
 Lower expression and hypermethylation of 5A in lung cancer cells.
42:19 108.
 5A suppressed proliferation in lung cancer cells.
42:22 109.
 SEMA5A-regulated genes were involved in cell growth and proliferation
42:23 110.
 Down-regulation of SEMA5A in lung adenocarcinoma tissues and cell lines
42:28 111.
 SEMA5A-regulated genes were involved in cell growth and proliferation
42:29 112.
 5A suppressed proliferation in lung cancer cells.
42:30 113.
 Lower expression and hypermethylation of 5A in lung cancer cells.
43:13 114.
 5A suppressed proliferation in lung cancer cells.
44:08 115.
 Lower expression and hypermethylation of 5A in lung cancer cells.
46:03 116.
 5A suppressed proliferation in lung cancer cells.
46:04 117.
 SEMA5A-regulated genes were involved in cell growth and proliferation
46:05 118.
 Down-regulation of SEMA5A in lung adenocarcinoma tissues and cell lines
46:11 119.
 Introduction of SEMA5A
48:37 120.
 Down-regulation of SEMA5A in lung adenocarcinoma tissues and cell lines
48:38 121.
 SEMA5A-regulated genes were involved in cell growth and proliferation
48:38 122.
 5A suppressed proliferation in lung cancer cells.
48:39 123.
 Lower expression and hypermethylation of 5A in lung cancer cells.
48:39 124.
 Summary of chapter 2
50:23 125.
 Lower expression and hypermethylation of 5A in lung cancer cells.
50:24 126.
 Summary of chapter 2
50:24 127.
銅葉綠素…
50:25 128.
 Introduction of HPLC-PCA
50:25 129.
Slide 11
50:25 130.
 Introduction of HPLC-PCA
50:26 131.
銅葉綠素…
50:26 132.
 Summary of chapter 2
50:27 133.
 Lower expression and hypermethylation of 5A in lung cancer cells.
50:27 134.
 5A suppressed proliferation in lung cancer cells.
52:11 135.
 Lower expression and hypermethylation of 5A in lung cancer cells.
52:12 136.
 Summary of chapter 2
52:13 137.
銅葉綠素…
52:17 138.
 Summary of chapter 2
52:37 139.
銅葉綠素…
53:40 140.
 Introduction of HPLC-PCA
53:43 141.
銅葉綠素…
54:57 142.
 Introduction of HPLC-PCA
55:00 143.
Slide 11
55:03 144.
 Chapter 4: Genomic approaches on Chinese medicine concept--- “qi”
1:01:13 145.
Slide 11
1:02:41 146.
 Chapter 4: Genomic approaches on Chinese medicine concept--- “qi”
1:04:03 147.
 Internal transcribed spacer (ITS)
1:04:03 148.
 Viability test of crudes on cancerous cells
1:04:04 149.
Slide 15
1:04:04 150.
 Markers identification
1:04:05 151.
 Markers identification
1:04:05 152.
 Summary of chapter 3 and 4
1:04:06 153.
 Markers identification
1:04:07 154.
 Summary of chapter 3 and 4
1:04:07 155.
 Chapter : Genomic approaches on a newly synthesized small molecule compound, MCDC
1:04:27 156.
Slide 20
1:07:27 157.
 Synthesis scheme of 1-substituted carbazolyl-1,2,3,4-tetrahydro-β-carboline and carbazolyl-3,4-dihydro-β-carboline derivatives
1:07:28 158.
Slide 22
1:07:28 159.
Slide 23
1:07:29 160.
 IC50 of MCDC in A549 and MCF7 cells
1:07:30 161.
 MIF-MCDC virtual screen-idTarget
1:07:31 162.
 MIF-MCDC virtual screen-Discovery studio
1:07:32 163.
 MIF-MCDC in vitro-Hydrogen-deuterium exchange
1:07:32 164.
 MIF-MCDC in cell line model.
1:09:01 165.
 MIF-MCDC in cell line model.
1:09:10 166.
 MIF-MCDC in cell line model.
1:09:48 167.
 MIF-MCDC in cell line model.
1:09:49 168.
 MIF-MCDC in cell line model.
1:09:50 169.
 MIF-MCDC in vitro-Hydrogen-deuterium exchange
1:09:50 170.
 MIF-MCDC virtual screen-Discovery studio
1:09:50 171.
 MIF-MCDC virtual screen-idTarget
1:09:51 172.
 IC50 of MCDC in A549 and MCF7 cells
1:09:59 173.
Slide 23
1:10:00 174.
Slide 22
1:10:00 175.
Slide 23
1:12:09 176.
Slide 22
1:12:10 177.
 Synthesis scheme of 1-substituted carbazolyl-1,2,3,4-tetrahydro-β-carboline and carbazolyl-3,4-dihydro-β-carboline derivatives
1:12:31 178.
Slide 20
1:12:33 179.
 Synthesis scheme of 1-substituted carbazolyl-1,2,3,4-tetrahydro-β-carboline and carbazolyl-3,4-dihydro-β-carboline derivatives
1:12:34 180.
Slide 22
1:12:34 181.
Slide 23
1:13:25 182.
 IC50 of MCDC in A549 and MCF7 cells
1:13:38 183.
 MIF-MCDC virtual screen-idTarget
1:13:40 184.
 MIF-MCDC virtual screen-Discovery studio
1:13:41 185.
 MIF-MCDC in vitro-Hydrogen-deuterium exchange
1:13:46 186.
 MIF-MCDC in cell line model.
1:13:47 187.
 MIF-MCDC in cell line model.
1:13:47 188.
 MIF-MCDC in cell line model.
1:13:48 189.
 MIF-MCDC in cell line model.
1:13:51 190.
 MCDC inhibits cell cycle-related protein expression.
1:13:52 191.
 MCDC inhibits cell cycle-related protein expression.
1:14:06 192.
 MCDC inhibits cell cycle-related protein expression.
1:15:36 193.
 MIF-MCDC in cell line model.
1:15:36 194.
 MIF-MCDC in cell line model.
1:15:38 195.
 MIF-MCDC in vitro-Hydrogen-deuterium exchange
1:15:39 196.
 MIF-MCDC virtual screen-Discovery studio
1:15:41 197.
 MIF-MCDC virtual screen-idTarget
1:15:41 198.
 IC50 of MCDC in A549 and MCF7 cells
1:15:42 199.
Slide 23
1:15:52 200.
 IC50 of MCDC in A549 and MCF7 cells
1:15:53 201.
 MIF-MCDC virtual screen-idTarget
1:16:03 202.
 MIF-MCDC virtual screen-Discovery studio
1:16:25 203.
 MIF-MCDC in vitro-Hydrogen-deuterium exchange
1:20:00 204.
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討論

00:00 1.
index 1
00:21 2.
 Introduction
00:56 3.
 Introduction of SEMA5A
00:58 4.
 Introduction
01:01 5.
 Introduction of SEMA5A
02:51 6.
 Down-regulation of SEMA5A in lung adenocarcinoma tissues and cell lines
04:22 7.
 SEMA5A-regulated genes were involved in cell growth and proliferation
05:48 8.
 5A suppressed proliferation in lung cancer cells.
05:58 9.
 SEMA5A-regulated genes were involved in cell growth and proliferation
06:03 10.
 5A suppressed proliferation in lung cancer cells.
07:47 11.
 Lower expression and hypermethylation of 5A in lung cancer cells.
09:08 12.
 Summary of chapter 2
09:37 13.
銅葉綠素…
13:38 14.
 Introduction of HPLC-PCA
14:57 15.
Slide 11
15:41 16.
 Chapter 4: Genomic approaches on Chinese medicine concept--- “qi”
18:31 17.
 Internal transcribed spacer (ITS)
18:32 18.
 Chapter 4: Genomic approaches on Chinese medicine concept--- “qi”
18:34 19.
 Internal transcribed spacer (ITS)
19:28 20.
 Viability test of crudes on cancerous cells
19:31 21.
 Internal transcribed spacer (ITS)
19:35 22.
 Viability test of crudes on cancerous cells
20:02 23.
Slide 15
20:04 24.
 Viability test of crudes on cancerous cells
20:06 25.
Slide 15
20:23 26.
 Markers identification
21:21 27.
 Markers identification
21:46 28.
 Summary of chapter 3 and 4
22:09 29.
 Chapter : Genomic approaches on a newly synthesized small molecule compound, MCDC
24:13 30.
Slide 20
25:13 31.
 Synthesis scheme of 1-substituted carbazolyl-1,2,3,4-tetrahydro-β-carboline and carbazolyl-3,4-dihydro-β-carboline derivatives
25:29 32.
Slide 22
25:59 33.
Slide 23
28:25 34.
 IC50 of MCDC in A549 and MCF7 cells
29:04 35.
 MIF-MCDC virtual screen-idTarget
30:27 36.
 MIF-MCDC virtual screen-Discovery studio
30:55 37.
 MIF-MCDC in vitro-Hydrogen-deuterium exchange
32:50 38.
 MIF-MCDC in cell line model.
33:19 39.
 MIF-MCDC in cell line model.
33:20 40.
 MIF-MCDC in cell line model.
34:03 41.
 MIF-MCDC in cell line model.
35:30 42.
 MCDC inhibits cell cycle-related protein expression.
36:28 43.
 MCDC inhibits cell cycle-related protein expression.
37:01 44.
 Summary of chapter 5
37:22 45.
 Manual adjustment of HPLC readings
37:26 46.
 Samples not applicable to ITS analysis
37:27 47.
Slide 35
37:27 48.
 Structures not provided in idTarget
37:28 49.
 Hydrophobicity of MCDC interfered the results of HDX and Biacore.
37:31 50.
 Endogenous MIF expression interfered U251 viability
37:31 51.
Slide 39
37:31 52.
Slide 40
37:32 53.
Slide 39
37:40 54.
 Endogenous MIF expression interfered U251 viability
37:41 55.
 Hydrophobicity of MCDC interfered the results of HDX and Biacore.
37:41 56.
 Structures not provided in idTarget
37:42 57.
Slide 35
37:42 58.
 Samples not applicable to ITS analysis
37:43 59.
 Manual adjustment of HPLC readings
37:43 60.
 Summary of chapter 5
37:43 61.
 MCDC inhibits cell cycle-related protein expression.
37:44 62.
 MCDC inhibits cell cycle-related protein expression.
37:44 63.
 MIF-MCDC in cell line model.
37:45 64.
 MIF-MCDC in cell line model.
37:45 65.
 MIF-MCDC in vitro-Hydrogen-deuterium exchange
37:46 66.
 MIF-MCDC virtual screen-Discovery studio
37:46 67.
 MIF-MCDC virtual screen-idTarget
37:46 68.
 IC50 of MCDC in A549 and MCF7 cells
37:47 69.
Slide 23
37:48 70.
Slide 22
37:48 71.
 Synthesis scheme of 1-substituted carbazolyl-1,2,3,4-tetrahydro-β-carboline and carbazolyl-3,4-dihydro-β-carboline derivatives
37:48 72.
Slide 20
37:49 73.
 Chapter : Genomic approaches on a newly synthesized small molecule compound, MCDC
37:49 74.
 Summary of chapter 3 and 4
37:50 75.
 Markers identification
37:50 76.
 Markers identification
37:50 77.
Slide 15
37:51 78.
 Viability test of crudes on cancerous cells
37:51 79.
 Internal transcribed spacer (ITS)
37:52 80.
 Chapter 4: Genomic approaches on Chinese medicine concept--- “qi”
37:52 81.
Slide 11
37:53 82.
 Introduction of HPLC-PCA
37:53 83.
銅葉綠素…
37:54 84.
 Summary of chapter 2
37:55 85.
 Lower expression and hypermethylation of 5A in lung cancer cells.
37:57 86.
 5A suppressed proliferation in lung cancer cells.
37:57 87.
 SEMA5A-regulated genes were involved in cell growth and proliferation
37:58 88.
 Down-regulation of SEMA5A in lung adenocarcinoma tissues and cell lines
37:58 89.
 Introduction of SEMA5A
37:59 90.
 Introduction
38:28 91.
 Introduction of SEMA5A
38:31 92.
 Down-regulation of SEMA5A in lung adenocarcinoma tissues and cell lines
38:33 93.
 SEMA5A-regulated genes were involved in cell growth and proliferation
38:37 94.
 5A suppressed proliferation in lung cancer cells.
38:48 95.
 SEMA5A-regulated genes were involved in cell growth and proliferation
38:49 96.
 Down-regulation of SEMA5A in lung adenocarcinoma tissues and cell lines
39:24 97.
 SEMA5A-regulated genes were involved in cell growth and proliferation
39:25 98.
 5A suppressed proliferation in lung cancer cells.
39:25 99.
 Lower expression and hypermethylation of 5A in lung cancer cells.
39:26 100.
 5A suppressed proliferation in lung cancer cells.
40:01 101.
 SEMA5A-regulated genes were involved in cell growth and proliferation
40:01 102.
 Down-regulation of SEMA5A in lung adenocarcinoma tissues and cell lines
40:02 103.
 Introduction of SEMA5A
40:03 104.
 Down-regulation of SEMA5A in lung adenocarcinoma tissues and cell lines
40:43 105.
 SEMA5A-regulated genes were involved in cell growth and proliferation
40:43 106.
 5A suppressed proliferation in lung cancer cells.
42:18 107.
 Lower expression and hypermethylation of 5A in lung cancer cells.
42:19 108.
 5A suppressed proliferation in lung cancer cells.
42:22 109.
 SEMA5A-regulated genes were involved in cell growth and proliferation
42:23 110.
 Down-regulation of SEMA5A in lung adenocarcinoma tissues and cell lines
42:28 111.
 SEMA5A-regulated genes were involved in cell growth and proliferation
42:29 112.
 5A suppressed proliferation in lung cancer cells.
42:30 113.
 Lower expression and hypermethylation of 5A in lung cancer cells.
43:13 114.
 5A suppressed proliferation in lung cancer cells.
44:08 115.
 Lower expression and hypermethylation of 5A in lung cancer cells.
46:03 116.
 5A suppressed proliferation in lung cancer cells.
46:04 117.
 SEMA5A-regulated genes were involved in cell growth and proliferation
46:05 118.
 Down-regulation of SEMA5A in lung adenocarcinoma tissues and cell lines
46:11 119.
 Introduction of SEMA5A
48:37 120.
 Down-regulation of SEMA5A in lung adenocarcinoma tissues and cell lines
48:38 121.
 SEMA5A-regulated genes were involved in cell growth and proliferation
48:38 122.
 5A suppressed proliferation in lung cancer cells.
48:39 123.
 Lower expression and hypermethylation of 5A in lung cancer cells.
48:39 124.
 Summary of chapter 2
50:23 125.
 Lower expression and hypermethylation of 5A in lung cancer cells.
50:24 126.
 Summary of chapter 2
50:24 127.
銅葉綠素…
50:25 128.
 Introduction of HPLC-PCA
50:25 129.
Slide 11
50:25 130.
 Introduction of HPLC-PCA
50:26 131.
銅葉綠素…
50:26 132.
 Summary of chapter 2
50:27 133.
 Lower expression and hypermethylation of 5A in lung cancer cells.
50:27 134.
 5A suppressed proliferation in lung cancer cells.
52:11 135.
 Lower expression and hypermethylation of 5A in lung cancer cells.
52:12 136.
 Summary of chapter 2
52:13 137.
銅葉綠素…
52:17 138.
 Summary of chapter 2
52:37 139.
銅葉綠素…
53:40 140.
 Introduction of HPLC-PCA
53:43 141.
銅葉綠素…
54:57 142.
 Introduction of HPLC-PCA
55:00 143.
Slide 11
55:03 144.
 Chapter 4: Genomic approaches on Chinese medicine concept--- “qi”
1:01:13 145.
Slide 11
1:02:41 146.
 Chapter 4: Genomic approaches on Chinese medicine concept--- “qi”
1:04:03 147.
 Internal transcribed spacer (ITS)
1:04:03 148.
 Viability test of crudes on cancerous cells
1:04:04 149.
Slide 15
1:04:04 150.
 Markers identification
1:04:05 151.
 Markers identification
1:04:05 152.
 Summary of chapter 3 and 4
1:04:06 153.
 Markers identification
1:04:07 154.
 Summary of chapter 3 and 4
1:04:07 155.
 Chapter : Genomic approaches on a newly synthesized small molecule compound, MCDC
1:04:27 156.
Slide 20
1:07:27 157.
 Synthesis scheme of 1-substituted carbazolyl-1,2,3,4-tetrahydro-β-carboline and carbazolyl-3,4-dihydro-β-carboline derivatives
1:07:28 158.
Slide 22
1:07:28 159.
Slide 23
1:07:29 160.
 IC50 of MCDC in A549 and MCF7 cells
1:07:30 161.
 MIF-MCDC virtual screen-idTarget
1:07:31 162.
 MIF-MCDC virtual screen-Discovery studio
1:07:32 163.
 MIF-MCDC in vitro-Hydrogen-deuterium exchange
1:07:32 164.
 MIF-MCDC in cell line model.
1:09:01 165.
 MIF-MCDC in cell line model.
1:09:10 166.
 MIF-MCDC in cell line model.
1:09:48 167.
 MIF-MCDC in cell line model.
1:09:49 168.
 MIF-MCDC in cell line model.
1:09:50 169.
 MIF-MCDC in vitro-Hydrogen-deuterium exchange
1:09:50 170.
 MIF-MCDC virtual screen-Discovery studio
1:09:50 171.
 MIF-MCDC virtual screen-idTarget
1:09:51 172.
 IC50 of MCDC in A549 and MCF7 cells
1:09:59 173.
Slide 23
1:10:00 174.
Slide 22
1:10:00 175.
Slide 23
1:12:09 176.
Slide 22
1:12:10 177.
 Synthesis scheme of 1-substituted carbazolyl-1,2,3,4-tetrahydro-β-carboline and carbazolyl-3,4-dihydro-β-carboline derivatives
1:12:31 178.
Slide 20
1:12:33 179.
 Synthesis scheme of 1-substituted carbazolyl-1,2,3,4-tetrahydro-β-carboline and carbazolyl-3,4-dihydro-β-carboline derivatives
1:12:34 180.
Slide 22
1:12:34 181.
Slide 23
1:13:25 182.
 IC50 of MCDC in A549 and MCF7 cells
1:13:38 183.
 MIF-MCDC virtual screen-idTarget
1:13:40 184.
 MIF-MCDC virtual screen-Discovery studio
1:13:41 185.
 MIF-MCDC in vitro-Hydrogen-deuterium exchange
1:13:46 186.
 MIF-MCDC in cell line model.
1:13:47 187.
 MIF-MCDC in cell line model.
1:13:47 188.
 MIF-MCDC in cell line model.
1:13:48 189.
 MIF-MCDC in cell line model.
1:13:51 190.
 MCDC inhibits cell cycle-related protein expression.
1:13:52 191.
 MCDC inhibits cell cycle-related protein expression.
1:14:06 192.
 MCDC inhibits cell cycle-related protein expression.
1:15:36 193.
 MIF-MCDC in cell line model.
1:15:36 194.
 MIF-MCDC in cell line model.
1:15:38 195.
 MIF-MCDC in vitro-Hydrogen-deuterium exchange
1:15:39 196.
 MIF-MCDC virtual screen-Discovery studio
1:15:41 197.
 MIF-MCDC virtual screen-idTarget
1:15:41 198.
 IC50 of MCDC in A549 and MCF7 cells
1:15:42 199.
Slide 23
1:15:52 200.
 IC50 of MCDC in A549 and MCF7 cells
1:15:53 201.
 MIF-MCDC virtual screen-idTarget
1:16:03 202.
 MIF-MCDC virtual screen-Discovery studio
1:16:25 203.
 MIF-MCDC in vitro-Hydrogen-deuterium exchange
1:20:00 204.
** after 博論-品豪.pptx

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20190701-lab meeting_品豪

長度: 1:20:05, 瀏覽: 804, 最近修訂: 2019-07-03

00:00 1.
index 1
00:21 2.
 Introduction
00:56 3.
 Introduction of SEMA5A
00:58 4.
 Introduction
01:01 5.
 Introduction of SEMA5A
02:51 6.
 Down-regulation of SEMA5A in lung adenocarcinoma tissues and cell lines
04:22 7.
 SEMA5A-regulated genes were involved in cell growth and proliferation
05:48 8.
 5A suppressed proliferation in lung cancer cells.
05:58 9.
 SEMA5A-regulated genes were involved in cell growth and proliferation
06:03 10.
 5A suppressed proliferation in lung cancer cells.
07:47 11.
 Lower expression and hypermethylation of 5A in lung cancer cells.
09:08 12.
 Summary of chapter 2
09:37 13.
銅葉綠素…
13:38 14.
 Introduction of HPLC-PCA
14:57 15.
Slide 11
15:41 16.
 Chapter 4: Genomic approaches on Chinese medicine concept--- “qi”
18:31 17.
 Internal transcribed spacer (ITS)
18:32 18.
 Chapter 4: Genomic approaches on Chinese medicine concept--- “qi”
18:34 19.
 Internal transcribed spacer (ITS)
19:28 20.
 Viability test of crudes on cancerous cells
19:31 21.
 Internal transcribed spacer (ITS)
19:35 22.
 Viability test of crudes on cancerous cells
20:02 23.
Slide 15
20:04 24.
 Viability test of crudes on cancerous cells
20:06 25.
Slide 15
20:23 26.
 Markers identification
21:21 27.
 Markers identification
21:46 28.
 Summary of chapter 3 and 4
22:09 29.
 Chapter : Genomic approaches on a newly synthesized small molecule compound, MCDC
24:13 30.
Slide 20
25:13 31.
 Synthesis scheme of 1-substituted carbazolyl-1,2,3,4-tetrahydro-β-carboline and carbazolyl-3,4-dihydro-β-carboline derivatives
25:29 32.
Slide 22
25:59 33.
Slide 23
28:25 34.
 IC50 of MCDC in A549 and MCF7 cells
29:04 35.
 MIF-MCDC virtual screen-idTarget
30:27 36.
 MIF-MCDC virtual screen-Discovery studio
30:55 37.
 MIF-MCDC in vitro-Hydrogen-deuterium exchange
32:50 38.
 MIF-MCDC in cell line model.
33:19 39.
 MIF-MCDC in cell line model.
33:20 40.
 MIF-MCDC in cell line model.
34:03 41.
 MIF-MCDC in cell line model.
35:30 42.
 MCDC inhibits cell cycle-related protein expression.
36:28 43.
 MCDC inhibits cell cycle-related protein expression.
37:01 44.
 Summary of chapter 5
37:22 45.
 Manual adjustment of HPLC readings
37:26 46.
 Samples not applicable to ITS analysis
37:27 47.
Slide 35
37:27 48.
 Structures not provided in idTarget
37:28 49.
 Hydrophobicity of MCDC interfered the results of HDX and Biacore.
37:31 50.
 Endogenous MIF expression interfered U251 viability
37:31 51.
Slide 39
37:31 52.
Slide 40
37:32 53.
Slide 39
37:40 54.
 Endogenous MIF expression interfered U251 viability
37:41 55.
 Hydrophobicity of MCDC interfered the results of HDX and Biacore.
37:41 56.
 Structures not provided in idTarget
37:42 57.
Slide 35
37:42 58.
 Samples not applicable to ITS analysis
37:43 59.
 Manual adjustment of HPLC readings
37:43 60.
 Summary of chapter 5
37:43 61.
 MCDC inhibits cell cycle-related protein expression.
37:44 62.
 MCDC inhibits cell cycle-related protein expression.
37:44 63.
 MIF-MCDC in cell line model.
37:45 64.
 MIF-MCDC in cell line model.
37:45 65.
 MIF-MCDC in vitro-Hydrogen-deuterium exchange
37:46 66.
 MIF-MCDC virtual screen-Discovery studio
37:46 67.
 MIF-MCDC virtual screen-idTarget
37:46 68.
 IC50 of MCDC in A549 and MCF7 cells
37:47 69.
Slide 23
37:48 70.
Slide 22
37:48 71.
 Synthesis scheme of 1-substituted carbazolyl-1,2,3,4-tetrahydro-β-carboline and carbazolyl-3,4-dihydro-β-carboline derivatives
37:48 72.
Slide 20
37:49 73.
 Chapter : Genomic approaches on a newly synthesized small molecule compound, MCDC
37:49 74.
 Summary of chapter 3 and 4
37:50 75.
 Markers identification
37:50 76.
 Markers identification
37:50 77.
Slide 15
37:51 78.
 Viability test of crudes on cancerous cells
37:51 79.
 Internal transcribed spacer (ITS)
37:52 80.
 Chapter 4: Genomic approaches on Chinese medicine concept--- “qi”
37:52 81.
Slide 11
37:53 82.
 Introduction of HPLC-PCA
37:53 83.
銅葉綠素…
37:54 84.
 Summary of chapter 2
37:55 85.
 Lower expression and hypermethylation of 5A in lung cancer cells.
37:57 86.
 5A suppressed proliferation in lung cancer cells.
37:57 87.
 SEMA5A-regulated genes were involved in cell growth and proliferation
37:58 88.
 Down-regulation of SEMA5A in lung adenocarcinoma tissues and cell lines
37:58 89.
 Introduction of SEMA5A
37:59 90.
 Introduction
38:28 91.
 Introduction of SEMA5A
38:31 92.
 Down-regulation of SEMA5A in lung adenocarcinoma tissues and cell lines
38:33 93.
 SEMA5A-regulated genes were involved in cell growth and proliferation
38:37 94.
 5A suppressed proliferation in lung cancer cells.
38:48 95.
 SEMA5A-regulated genes were involved in cell growth and proliferation
38:49 96.
 Down-regulation of SEMA5A in lung adenocarcinoma tissues and cell lines
39:24 97.
 SEMA5A-regulated genes were involved in cell growth and proliferation
39:25 98.
 5A suppressed proliferation in lung cancer cells.
39:25 99.
 Lower expression and hypermethylation of 5A in lung cancer cells.
39:26 100.
 5A suppressed proliferation in lung cancer cells.
40:01 101.
 SEMA5A-regulated genes were involved in cell growth and proliferation
40:01 102.
 Down-regulation of SEMA5A in lung adenocarcinoma tissues and cell lines
40:02 103.
 Introduction of SEMA5A
40:03 104.
 Down-regulation of SEMA5A in lung adenocarcinoma tissues and cell lines
40:43 105.
 SEMA5A-regulated genes were involved in cell growth and proliferation
40:43 106.
 5A suppressed proliferation in lung cancer cells.
42:18 107.
 Lower expression and hypermethylation of 5A in lung cancer cells.
42:19 108.
 5A suppressed proliferation in lung cancer cells.
42:22 109.
 SEMA5A-regulated genes were involved in cell growth and proliferation
42:23 110.
 Down-regulation of SEMA5A in lung adenocarcinoma tissues and cell lines
42:28 111.
 SEMA5A-regulated genes were involved in cell growth and proliferation
42:29 112.
 5A suppressed proliferation in lung cancer cells.
42:30 113.
 Lower expression and hypermethylation of 5A in lung cancer cells.
43:13 114.
 5A suppressed proliferation in lung cancer cells.
44:08 115.
 Lower expression and hypermethylation of 5A in lung cancer cells.
46:03 116.
 5A suppressed proliferation in lung cancer cells.
46:04 117.
 SEMA5A-regulated genes were involved in cell growth and proliferation
46:05 118.
 Down-regulation of SEMA5A in lung adenocarcinoma tissues and cell lines
46:11 119.
 Introduction of SEMA5A
48:37 120.
 Down-regulation of SEMA5A in lung adenocarcinoma tissues and cell lines
48:38 121.
 SEMA5A-regulated genes were involved in cell growth and proliferation
48:38 122.
 5A suppressed proliferation in lung cancer cells.
48:39 123.
 Lower expression and hypermethylation of 5A in lung cancer cells.
48:39 124.
 Summary of chapter 2
50:23 125.
 Lower expression and hypermethylation of 5A in lung cancer cells.
50:24 126.
 Summary of chapter 2
50:24 127.
銅葉綠素…
50:25 128.
 Introduction of HPLC-PCA
50:25 129.
Slide 11
50:25 130.
 Introduction of HPLC-PCA
50:26 131.
銅葉綠素…
50:26 132.
 Summary of chapter 2
50:27 133.
 Lower expression and hypermethylation of 5A in lung cancer cells.
50:27 134.
 5A suppressed proliferation in lung cancer cells.
52:11 135.
 Lower expression and hypermethylation of 5A in lung cancer cells.
52:12 136.
 Summary of chapter 2
52:13 137.
銅葉綠素…
52:17 138.
 Summary of chapter 2
52:37 139.
銅葉綠素…
53:40 140.
 Introduction of HPLC-PCA
53:43 141.
銅葉綠素…
54:57 142.
 Introduction of HPLC-PCA
55:00 143.
Slide 11
55:03 144.
 Chapter 4: Genomic approaches on Chinese medicine concept--- “qi”
1:01:13 145.
Slide 11
1:02:41 146.
 Chapter 4: Genomic approaches on Chinese medicine concept--- “qi”
1:04:03 147.
 Internal transcribed spacer (ITS)
1:04:03 148.
 Viability test of crudes on cancerous cells
1:04:04 149.
Slide 15
1:04:04 150.
 Markers identification
1:04:05 151.
 Markers identification
1:04:05 152.
 Summary of chapter 3 and 4
1:04:06 153.
 Markers identification
1:04:07 154.
 Summary of chapter 3 and 4
1:04:07 155.
 Chapter : Genomic approaches on a newly synthesized small molecule compound, MCDC
1:04:27 156.
Slide 20
1:07:27 157.
 Synthesis scheme of 1-substituted carbazolyl-1,2,3,4-tetrahydro-β-carboline and carbazolyl-3,4-dihydro-β-carboline derivatives
1:07:28 158.
Slide 22
1:07:28 159.
Slide 23
1:07:29 160.
 IC50 of MCDC in A549 and MCF7 cells
1:07:30 161.
 MIF-MCDC virtual screen-idTarget
1:07:31 162.
 MIF-MCDC virtual screen-Discovery studio
1:07:32 163.
 MIF-MCDC in vitro-Hydrogen-deuterium exchange
1:07:32 164.
 MIF-MCDC in cell line model.
1:09:01 165.
 MIF-MCDC in cell line model.
1:09:10 166.
 MIF-MCDC in cell line model.
1:09:48 167.
 MIF-MCDC in cell line model.
1:09:49 168.
 MIF-MCDC in cell line model.
1:09:50 169.
 MIF-MCDC in vitro-Hydrogen-deuterium exchange
1:09:50 170.
 MIF-MCDC virtual screen-Discovery studio
1:09:50 171.
 MIF-MCDC virtual screen-idTarget
1:09:51 172.
 IC50 of MCDC in A549 and MCF7 cells
1:09:59 173.
Slide 23
1:10:00 174.
Slide 22
1:10:00 175.
Slide 23
1:12:09 176.
Slide 22
1:12:10 177.
 Synthesis scheme of 1-substituted carbazolyl-1,2,3,4-tetrahydro-β-carboline and carbazolyl-3,4-dihydro-β-carboline derivatives
1:12:31 178.
Slide 20
1:12:33 179.
 Synthesis scheme of 1-substituted carbazolyl-1,2,3,4-tetrahydro-β-carboline and carbazolyl-3,4-dihydro-β-carboline derivatives
1:12:34 180.
Slide 22
1:12:34 181.
Slide 23
1:13:25 182.
 IC50 of MCDC in A549 and MCF7 cells
1:13:38 183.
 MIF-MCDC virtual screen-idTarget
1:13:40 184.
 MIF-MCDC virtual screen-Discovery studio
1:13:41 185.
 MIF-MCDC in vitro-Hydrogen-deuterium exchange
1:13:46 186.
 MIF-MCDC in cell line model.
1:13:47 187.
 MIF-MCDC in cell line model.
1:13:47 188.
 MIF-MCDC in cell line model.
1:13:48 189.
 MIF-MCDC in cell line model.
1:13:51 190.
 MCDC inhibits cell cycle-related protein expression.
1:13:52 191.
 MCDC inhibits cell cycle-related protein expression.
1:14:06 192.
 MCDC inhibits cell cycle-related protein expression.
1:15:36 193.
 MIF-MCDC in cell line model.
1:15:36 194.
 MIF-MCDC in cell line model.
1:15:38 195.
 MIF-MCDC in vitro-Hydrogen-deuterium exchange
1:15:39 196.
 MIF-MCDC virtual screen-Discovery studio
1:15:41 197.
 MIF-MCDC virtual screen-idTarget
1:15:41 198.
 IC50 of MCDC in A549 and MCF7 cells
1:15:42 199.
Slide 23
1:15:52 200.
 IC50 of MCDC in A549 and MCF7 cells
1:15:53 201.
 MIF-MCDC virtual screen-idTarget
1:16:03 202.
 MIF-MCDC virtual screen-Discovery studio
1:16:25 203.
 MIF-MCDC in vitro-Hydrogen-deuterium exchange
1:20:00 204.
** after 博論-品豪.pptx

位置

資料夾名稱

2019

發表人

李冠儀

單位

賴亮全教授

建立

2019-07-03 12:08:14

最近修訂

2019-07-03 14:15:26

長度

1:20:05