Immunohistochemistry | 賴亮全實驗室

Index

00:24 1.
Immunohistochemistry
00:08 2.
Deparaffinization: Xylene I
00:14 3.
Deparaffinization: Xylene II
00:14 4.
Hydration 100% EtOH
00:12 5.
Hydration 95% EtOH
00:12 6.
Hydration 80% EtOH
00:15 7.
Hydration 60% EtOH
00:05 8.
Wash 3 times with H2O (1 min per time)
00:06 9.
Antigen Retrievel: Citrate buffer, pH6
00:02 9.1
Medium heat, 3 min
00:04 9.2
Cool 30-40 min
00:09 10.
Rinse with TBS buffer
00:09 11.
Block endogenous Peroxidase: add 3% H2O2 solution
00:06 11.1
Place in a closed dark container
00:27 12.
Rinse with TBS buffer
00:05 13.
Serum blocking: add 3% BSA solution
00:05 13.1
Place in a closed dark container
00:04 14.
Remove excess BSA
00:05 15.
Immunosatain: add diluted primary antibody
00:03 15.1
Place in a closed dark container
00:17 16.
Rinse with TBST buffer
00:14 17.
Add diluted 2nd antibody
00:21 18.
Rinse with TBS buffer
00:10 19.
Add DAB staining solution
00:13 20.
Rinse with H2O
00:10 21.
Countstain with Mayer's Haematoxylin
00:17 22.
Rinse with H2O
00:07 23.
Place in 1% HCl ethanol
00:10 24.
Rinse with H2O
00:06 25.
Dehadration: 60% EtOH
00:10 26.
Dehydation: 80% EtOH
00:13 27.
Dehydation: 95% EtOH
00:13 28.
Dehydation: 100% EtOH
00:16 29.
Dehydation: Xylene III
00:21 30.
Dehydation: Xylene IV
00:18 31.
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00:24 1.
Immunohistochemistry
00:08 2.
Deparaffinization: Xylene I
00:14 3.
Deparaffinization: Xylene II
00:14 4.
Hydration 100% EtOH
00:12 5.
Hydration 95% EtOH
00:12 6.
Hydration 80% EtOH
00:15 7.
Hydration 60% EtOH
00:05 8.
Wash 3 times with H2O (1 min per time)
00:06 9.
Antigen Retrievel: Citrate buffer, pH6
00:02 9.1
Medium heat, 3 min
00:04 9.2
Cool 30-40 min
00:09 10.
Rinse with TBS buffer
00:09 11.
Block endogenous Peroxidase: add 3% H2O2 solution
00:06 11.1
Place in a closed dark container
00:27 12.
Rinse with TBS buffer
00:05 13.
Serum blocking: add 3% BSA solution
00:05 13.1
Place in a closed dark container
00:04 14.
Remove excess BSA
00:05 15.
Immunosatain: add diluted primary antibody
00:03 15.1
Place in a closed dark container
00:17 16.
Rinse with TBST buffer
00:14 17.
Add diluted 2nd antibody
00:21 18.
Rinse with TBS buffer
00:10 19.
Add DAB staining solution
00:13 20.
Rinse with H2O
00:10 21.
Countstain with Mayer's Haematoxylin
00:17 22.
Rinse with H2O
00:07 23.
Place in 1% HCl ethanol
00:10 24.
Rinse with H2O
00:06 25.
Dehadration: 60% EtOH
00:10 26.
Dehydation: 80% EtOH
00:13 27.
Dehydation: 95% EtOH
00:13 28.
Dehydation: 100% EtOH
00:16 29.
Dehydation: Xylene III
00:21 30.
Dehydation: Xylene IV
00:18 31.
Seal slides

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Immunohistochemistry

Duration: 07:00, Browse: 726, Last Updated: 2018-12-03

Next

00:24 1.
Immunohistochemistry
00:08 2.
Deparaffinization: Xylene I
00:14 3.
Deparaffinization: Xylene II
00:14 4.
Hydration 100% EtOH
00:12 5.
Hydration 95% EtOH
00:12 6.
Hydration 80% EtOH
00:15 7.
Hydration 60% EtOH
00:05 8.
Wash 3 times with H2O (1 min per time)
00:06 9.
Antigen Retrievel: Citrate buffer, pH6
00:02 9.1
Medium heat, 3 min
00:04 9.2
Cool 30-40 min
00:09 10.
Rinse with TBS buffer
00:09 11.
Block endogenous Peroxidase: add 3% H2O2 solution
00:06 11.1
Place in a closed dark container
00:27 12.
Rinse with TBS buffer
00:05 13.
Serum blocking: add 3% BSA solution
00:05 13.1
Place in a closed dark container
00:04 14.
Remove excess BSA
00:05 15.
Immunosatain: add diluted primary antibody
00:03 15.1
Place in a closed dark container
00:17 16.
Rinse with TBST buffer
00:14 17.
Add diluted 2nd antibody
00:21 18.
Rinse with TBS buffer
00:10 19.
Add DAB staining solution
00:13 20.
Rinse with H2O
00:10 21.
Countstain with Mayer's Haematoxylin
00:17 22.
Rinse with H2O
00:07 23.
Place in 1% HCl ethanol
00:10 24.
Rinse with H2O
00:06 25.
Dehadration: 60% EtOH
00:10 26.
Dehydation: 80% EtOH
00:13 27.
Dehydation: 95% EtOH
00:13 28.
Dehydation: 100% EtOH
00:16 29.
Dehydation: Xylene III
00:21 30.
Dehydation: Xylene IV
00:18 31.
Seal slides

Location

Folder name

Immunohistochemistry (免疫組織染色)

Author

系統管理者

Branch

賴亮全教授

Created

2018-12-03 11:20:21

Last Updated

2018-12-03 11:34:07

Browse

726

Duration

07:00