-
00:24
1.
Immunohistochemistry
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00:08
2.
Deparaffinization: Xylene I
-
00:14
3.
Deparaffinization: Xylene II
-
00:14
4.
Hydration 100% EtOH
-
00:12
5.
Hydration 95% EtOH
-
00:12
6.
Hydration 80% EtOH
-
00:15
7.
Hydration 60% EtOH
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00:05
8.
Wash 3 times with H2O (1 min per time)
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00:06
9.
Antigen Retrievel: Citrate buffer, pH6
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00:02
9.1
Medium heat, 3 min
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00:04
9.2
Cool 30-40 min
-
00:09
10.
Rinse with TBS buffer
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00:09
11.
Block endogenous Peroxidase: add 3% H2O2 solution
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00:06
11.1
Place in a closed dark container
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00:27
12.
Rinse with TBS buffer
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00:05
13.
Serum blocking: add 3% BSA solution
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00:05
13.1
Place in a closed dark container
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00:04
14.
Remove excess BSA
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00:05
15.
Immunosatain: add diluted primary antibody
-
00:03
15.1
Place in a closed dark container
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00:17
16.
Rinse with TBST buffer
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00:14
17.
Add diluted 2nd antibody
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00:21
18.
Rinse with TBS buffer
-
00:10
19.
Add DAB staining solution
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00:13
20.
Rinse with H2O
-
00:10
21.
Countstain with Mayer's Haematoxylin
-
00:17
22.
Rinse with H2O
-
00:07
23.
Place in 1% HCl ethanol
-
00:10
24.
Rinse with H2O
-
00:06
25.
Dehadration: 60% EtOH
-
00:10
26.
Dehydation: 80% EtOH
-
00:13
27.
Dehydation: 95% EtOH
-
00:13
28.
Dehydation: 100% EtOH
-
00:16
29.
Dehydation: Xylene III
-
00:21
30.
Dehydation: Xylene IV
-
00:18
31.
Seal slides