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00:00
1.
Immunohistochemistry
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00:24
2.
Deparaffinization: Xylene I
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00:32
3.
Deparaffinization: Xylene II
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00:46
4.
Hydration 100% EtOH
-
01:00
5.
Hydration 95% EtOH
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01:12
6.
Hydration 80% EtOH
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01:24
7.
Hydration 60% EtOH
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01:40
8.
Wash 3 times with H2O (1 min per time)
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01:45
9.
Antigen Retrievel: Citrate buffer, pH6
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01:51
9.1
Medium heat, 3 min
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01:54
9.2
Cool 30-40 min
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01:59
10.
Rinse with TBS buffer
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02:08
11.
Block endogenous Peroxidase: add 3% H2O2 solution
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02:18
11.1
Place in a closed dark container
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02:24
12.
Rinse with TBS buffer
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02:52
13.
Serum blocking: add 3% BSA solution
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02:57
13.1
Place in a closed dark container
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03:02
14.
Remove excess BSA
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03:07
15.
Immunosatain: add diluted primary antibody
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03:12
15.1
Place in a closed dark container
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03:15
16.
Rinse with TBST buffer
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03:32
17.
Add diluted 2nd antibody
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03:47
18.
Rinse with TBS buffer
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04:08
19.
Add DAB staining solution
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04:19
20.
Rinse with H2O
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04:32
21.
Countstain with Mayer's Haematoxylin
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04:42
22.
Rinse with H2O
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05:00
23.
Place in 1% HCl ethanol
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05:07
24.
Rinse with H2O
-
05:17
25.
Dehadration: 60% EtOH
-
05:24
26.
Dehydation: 80% EtOH
-
05:35
27.
Dehydation: 95% EtOH
-
05:49
28.
Dehydation: 100% EtOH
-
06:02
29.
Dehydation: Xylene III
-
06:19
30.
Dehydation: Xylene IV
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06:41
31.
Seal slides