Immunohistochemistry | 賴亮全實驗室

Index

00:00 1.
Immunohistochemistry
00:24 2.
Deparaffinization: Xylene I
00:32 3.
Deparaffinization: Xylene II
00:46 4.
Hydration 100% EtOH
01:00 5.
Hydration 95% EtOH
01:12 6.
Hydration 80% EtOH
01:24 7.
Hydration 60% EtOH
01:40 8.
Wash 3 times with H2O (1 min per time)
01:45 9.
Antigen Retrievel: Citrate buffer, pH6
01:51 9.1
Medium heat, 3 min
01:54 9.2
Cool 30-40 min
01:59 10.
Rinse with TBS buffer
02:08 11.
Block endogenous Peroxidase: add 3% H2O2 solution
02:18 11.1
Place in a closed dark container
02:24 12.
Rinse with TBS buffer
02:52 13.
Serum blocking: add 3% BSA solution
02:57 13.1
Place in a closed dark container
03:02 14.
Remove excess BSA
03:07 15.
Immunosatain: add diluted primary antibody
03:12 15.1
Place in a closed dark container
03:15 16.
Rinse with TBST buffer
03:32 17.
Add diluted 2nd antibody
03:47 18.
Rinse with TBS buffer
04:08 19.
Add DAB staining solution
04:19 20.
Rinse with H2O
04:32 21.
Countstain with Mayer's Haematoxylin
04:42 22.
Rinse with H2O
05:00 23.
Place in 1% HCl ethanol
05:07 24.
Rinse with H2O
05:17 25.
Dehadration: 60% EtOH
05:24 26.
Dehydation: 80% EtOH
05:35 27.
Dehydation: 95% EtOH
05:49 28.
Dehydation: 100% EtOH
06:02 29.
Dehydation: Xylene III
06:19 30.
Dehydation: Xylene IV
06:41 31.
Seal slides

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Comment

00:00 1.
Immunohistochemistry
00:24 2.
Deparaffinization: Xylene I
00:32 3.
Deparaffinization: Xylene II
00:46 4.
Hydration 100% EtOH
01:00 5.
Hydration 95% EtOH
01:12 6.
Hydration 80% EtOH
01:24 7.
Hydration 60% EtOH
01:40 8.
Wash 3 times with H2O (1 min per time)
01:45 9.
Antigen Retrievel: Citrate buffer, pH6
01:51 9.1
Medium heat, 3 min
01:54 9.2
Cool 30-40 min
01:59 10.
Rinse with TBS buffer
02:08 11.
Block endogenous Peroxidase: add 3% H2O2 solution
02:18 11.1
Place in a closed dark container
02:24 12.
Rinse with TBS buffer
02:52 13.
Serum blocking: add 3% BSA solution
02:57 13.1
Place in a closed dark container
03:02 14.
Remove excess BSA
03:07 15.
Immunosatain: add diluted primary antibody
03:12 15.1
Place in a closed dark container
03:15 16.
Rinse with TBST buffer
03:32 17.
Add diluted 2nd antibody
03:47 18.
Rinse with TBS buffer
04:08 19.
Add DAB staining solution
04:19 20.
Rinse with H2O
04:32 21.
Countstain with Mayer's Haematoxylin
04:42 22.
Rinse with H2O
05:00 23.
Place in 1% HCl ethanol
05:07 24.
Rinse with H2O
05:17 25.
Dehadration: 60% EtOH
05:24 26.
Dehydation: 80% EtOH
05:35 27.
Dehydation: 95% EtOH
05:49 28.
Dehydation: 100% EtOH
06:02 29.
Dehydation: Xylene III
06:19 30.
Dehydation: Xylene IV
06:41 31.
Seal slides

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Immunohistochemistry

Duration: 07:00, Browse: 977, Last Updated: 2018-12-03

Next

00:00 1.
Immunohistochemistry
00:24 2.
Deparaffinization: Xylene I
00:32 3.
Deparaffinization: Xylene II
00:46 4.
Hydration 100% EtOH
01:00 5.
Hydration 95% EtOH
01:12 6.
Hydration 80% EtOH
01:24 7.
Hydration 60% EtOH
01:40 8.
Wash 3 times with H2O (1 min per time)
01:45 9.
Antigen Retrievel: Citrate buffer, pH6
01:51 9.1
Medium heat, 3 min
01:54 9.2
Cool 30-40 min
01:59 10.
Rinse with TBS buffer
02:08 11.
Block endogenous Peroxidase: add 3% H2O2 solution
02:18 11.1
Place in a closed dark container
02:24 12.
Rinse with TBS buffer
02:52 13.
Serum blocking: add 3% BSA solution
02:57 13.1
Place in a closed dark container
03:02 14.
Remove excess BSA
03:07 15.
Immunosatain: add diluted primary antibody
03:12 15.1
Place in a closed dark container
03:15 16.
Rinse with TBST buffer
03:32 17.
Add diluted 2nd antibody
03:47 18.
Rinse with TBS buffer
04:08 19.
Add DAB staining solution
04:19 20.
Rinse with H2O
04:32 21.
Countstain with Mayer's Haematoxylin
04:42 22.
Rinse with H2O
05:00 23.
Place in 1% HCl ethanol
05:07 24.
Rinse with H2O
05:17 25.
Dehadration: 60% EtOH
05:24 26.
Dehydation: 80% EtOH
05:35 27.
Dehydation: 95% EtOH
05:49 28.
Dehydation: 100% EtOH
06:02 29.
Dehydation: Xylene III
06:19 30.
Dehydation: Xylene IV
06:41 31.
Seal slides

Location

Folder name

Immunohistochemistry (免疫組織染色)

Author

系統管理者

Branch

賴亮全教授

Created

2018-12-03 11:20:21

Last Updated

2018-12-03 11:34:07

Duration

07:00