• 00:00 1.
    Immunohistochemistry
  • 00:24 2.
    Deparaffinization: Xylene I
  • 00:32 3.
    Deparaffinization: Xylene II
  • 00:46 4.
    Hydration 100% EtOH
  • 01:00 5.
    Hydration 95% EtOH
  • 01:12 6.
    Hydration 80% EtOH
  • 01:24 7.
    Hydration 60% EtOH
  • 01:40 8.
    Wash 3 times with H2O (1 min per time)
  • 01:45 9.
    Antigen Retrievel: Citrate buffer, pH6
  • 01:51 9.1
    Medium heat, 3 min
  • 01:54 9.2
    Cool 30-40 min
  • 01:59 10.
    Rinse with TBS buffer
  • 02:08 11.
    Block endogenous Peroxidase: add 3% H2O2 solution
  • 02:18 11.1
    Place in a closed dark container
  • 02:24 12.
    Rinse with TBS buffer
  • 02:52 13.
    Serum blocking: add 3% BSA solution
  • 02:57 13.1
    Place in a closed dark container
  • 03:02 14.
    Remove excess BSA
  • 03:07 15.
    Immunosatain: add diluted primary antibody
  • 03:12 15.1
    Place in a closed dark container
  • 03:15 16.
    Rinse with TBST buffer
  • 03:32 17.
    Add diluted 2nd antibody
  • 03:47 18.
    Rinse with TBS buffer
  • 04:08 19.
    Add DAB staining solution
  • 04:19 20.
    Rinse with H2O
  • 04:32 21.
    Countstain with Mayer's Haematoxylin
  • 04:42 22.
    Rinse with H2O
  • 05:00 23.
    Place in 1% HCl ethanol
  • 05:07 24.
    Rinse with H2O
  • 05:17 25.
    Dehadration: 60% EtOH
  • 05:24 26.
    Dehydation: 80% EtOH
  • 05:35 27.
    Dehydation: 95% EtOH
  • 05:49 28.
    Dehydation: 100% EtOH
  • 06:02 29.
    Dehydation: Xylene III
  • 06:19 30.
    Dehydation: Xylene IV
  • 06:41 31.
    Seal slides
  • Index
  • Notes
  • Comment
  • Fullscreen
Immunohistochemistry
Duration: 07:00, Browse: 881, Last Updated: 2018-12-03
    • 00:00 1.
      Immunohistochemistry
    • 00:24 2.
      Deparaffinization: Xylene I
    • 00:32 3.
      Deparaffinization: Xylene II
    • 00:46 4.
      Hydration 100% EtOH
    • 01:00 5.
      Hydration 95% EtOH
    • 01:12 6.
      Hydration 80% EtOH
    • 01:24 7.
      Hydration 60% EtOH
    • 01:40 8.
      Wash 3 times with H2O (1 min per time)
    • 01:45 9.
      Antigen Retrievel: Citrate buffer, pH6
    • 01:51 9.1
      Medium heat, 3 min
    • 01:54 9.2
      Cool 30-40 min
    • 01:59 10.
      Rinse with TBS buffer
    • 02:08 11.
      Block endogenous Peroxidase: add 3% H2O2 solution
    • 02:18 11.1
      Place in a closed dark container
    • 02:24 12.
      Rinse with TBS buffer
    • 02:52 13.
      Serum blocking: add 3% BSA solution
    • 02:57 13.1
      Place in a closed dark container
    • 03:02 14.
      Remove excess BSA
    • 03:07 15.
      Immunosatain: add diluted primary antibody
    • 03:12 15.1
      Place in a closed dark container
    • 03:15 16.
      Rinse with TBST buffer
    • 03:32 17.
      Add diluted 2nd antibody
    • 03:47 18.
      Rinse with TBS buffer
    • 04:08 19.
      Add DAB staining solution
    • 04:19 20.
      Rinse with H2O
    • 04:32 21.
      Countstain with Mayer's Haematoxylin
    • 04:42 22.
      Rinse with H2O
    • 05:00 23.
      Place in 1% HCl ethanol
    • 05:07 24.
      Rinse with H2O
    • 05:17 25.
      Dehadration: 60% EtOH
    • 05:24 26.
      Dehydation: 80% EtOH
    • 05:35 27.
      Dehydation: 95% EtOH
    • 05:49 28.
      Dehydation: 100% EtOH
    • 06:02 29.
      Dehydation: Xylene III
    • 06:19 30.
      Dehydation: Xylene IV
    • 06:41 31.
      Seal slides
    Location
    Folder name
    Immunohistochemistry (免疫組織染色)
    Author
    系統管理者
    Branch
    賴亮全教授
    Created
    2018-12-03 11:20:21
    Last Updated
    2018-12-03 11:34:07
    Duration
    07:00