• 00:24 1.
    Immunohistochemistry
  • 00:08 2.
    Deparaffinization: Xylene I
  • 00:14 3.
    Deparaffinization: Xylene II
  • 00:14 4.
    Hydration 100% EtOH
  • 00:12 5.
    Hydration 95% EtOH
  • 00:12 6.
    Hydration 80% EtOH
  • 00:15 7.
    Hydration 60% EtOH
  • 00:05 8.
    Wash 3 times with H2O (1 min per time)
  • 00:06 9.
    Antigen Retrievel: Citrate buffer, pH6
  • 00:02 9.1
    Medium heat, 3 min
  • 00:04 9.2
    Cool 30-40 min
  • 00:09 10.
    Rinse with TBS buffer
  • 00:09 11.
    Block endogenous Peroxidase: add 3% H2O2 solution
  • 00:06 11.1
    Place in a closed dark container
  • 00:27 12.
    Rinse with TBS buffer
  • 00:05 13.
    Serum blocking: add 3% BSA solution
  • 00:05 13.1
    Place in a closed dark container
  • 00:04 14.
    Remove excess BSA
  • 00:05 15.
    Immunosatain: add diluted primary antibody
  • 00:03 15.1
    Place in a closed dark container
  • 00:17 16.
    Rinse with TBST buffer
  • 00:14 17.
    Add diluted 2nd antibody
  • 00:21 18.
    Rinse with TBS buffer
  • 00:10 19.
    Add DAB staining solution
  • 00:13 20.
    Rinse with H2O
  • 00:10 21.
    Countstain with Mayer's Haematoxylin
  • 00:17 22.
    Rinse with H2O
  • 00:07 23.
    Place in 1% HCl ethanol
  • 00:10 24.
    Rinse with H2O
  • 00:06 25.
    Dehadration: 60% EtOH
  • 00:10 26.
    Dehydation: 80% EtOH
  • 00:13 27.
    Dehydation: 95% EtOH
  • 00:13 28.
    Dehydation: 100% EtOH
  • 00:16 29.
    Dehydation: Xylene III
  • 00:21 30.
    Dehydation: Xylene IV
  • 00:18 31.
    Seal slides
  • Index
  • Notes
  • Comment
  • Fullscreen
Immunohistochemistry
Duration: 07:00, Browse: 645, Last Updated: 2018-12-03
    Next
    • 00:24 1.
      Immunohistochemistry
    • 00:08 2.
      Deparaffinization: Xylene I
    • 00:14 3.
      Deparaffinization: Xylene II
    • 00:14 4.
      Hydration 100% EtOH
    • 00:12 5.
      Hydration 95% EtOH
    • 00:12 6.
      Hydration 80% EtOH
    • 00:15 7.
      Hydration 60% EtOH
    • 00:05 8.
      Wash 3 times with H2O (1 min per time)
    • 00:06 9.
      Antigen Retrievel: Citrate buffer, pH6
    • 00:02 9.1
      Medium heat, 3 min
    • 00:04 9.2
      Cool 30-40 min
    • 00:09 10.
      Rinse with TBS buffer
    • 00:09 11.
      Block endogenous Peroxidase: add 3% H2O2 solution
    • 00:06 11.1
      Place in a closed dark container
    • 00:27 12.
      Rinse with TBS buffer
    • 00:05 13.
      Serum blocking: add 3% BSA solution
    • 00:05 13.1
      Place in a closed dark container
    • 00:04 14.
      Remove excess BSA
    • 00:05 15.
      Immunosatain: add diluted primary antibody
    • 00:03 15.1
      Place in a closed dark container
    • 00:17 16.
      Rinse with TBST buffer
    • 00:14 17.
      Add diluted 2nd antibody
    • 00:21 18.
      Rinse with TBS buffer
    • 00:10 19.
      Add DAB staining solution
    • 00:13 20.
      Rinse with H2O
    • 00:10 21.
      Countstain with Mayer's Haematoxylin
    • 00:17 22.
      Rinse with H2O
    • 00:07 23.
      Place in 1% HCl ethanol
    • 00:10 24.
      Rinse with H2O
    • 00:06 25.
      Dehadration: 60% EtOH
    • 00:10 26.
      Dehydation: 80% EtOH
    • 00:13 27.
      Dehydation: 95% EtOH
    • 00:13 28.
      Dehydation: 100% EtOH
    • 00:16 29.
      Dehydation: Xylene III
    • 00:21 30.
      Dehydation: Xylene IV
    • 00:18 31.
      Seal slides
    Location
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    3. Immunohistochemistry (免疫組織染色)
    Folder name
    Immunohistochemistry (免疫組織染色)
    Author
    系統管理者
    Branch
    賴亮全教授
    Created
    2018-12-03 11:20:21
    Last Updated
    2018-12-03 11:34:07
    Browse
    645
    Duration
    07:00