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Print date : 2023/09/22
賴亮全實驗室
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實驗室資料夾
質體清單
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pcDNA6.2-GW/EmGFP-miR-neg-miR-769-3p
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Browse: 525,
Last Updated: 2019-09-10
pcDNA6.2-GW/EmGFP-miR-neg-miR-769-3p
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Browse: 525,
Last Updated: 2019-09-10
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維持者: 幸華
存放位置: [-20C冰箱][3層][1櫃][Master box盒][?格]
*過去的質體清單有，但目前找不到存放位置
若要定序、畫map，需要重訂primer
我以其他backbone也是pcDNA6.2的質體map找primer序列
推估primer序列可以用AACCTCTTACGTGCCGATCAAC，但尚未訂購primer
Plasmid datasheet:
Parental Vector:
https://www.thermofisher.com/order/catalog/product/K493600
Gene (Insert):
http://www.mirbase.org/cgi-bin/mature.pl?mature_acc=MIMAT0003887
DNA sequencing: 需訂購primer並送定序才能得知
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Location
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實驗室資料夾
質體清單
Folder name
質體清單
Author
趙幸華
Branch
賴亮全教授
Created
2019-09-10 18:16:40
Last Updated
2019-09-10 18:22:48
Browse
525
1.
質體(plasmid/vector)清單
2.
巧宜 質體
2.1
pMIR-REPORT-DANCR
2.2
pMIR-REPORT-DANCR; 7328 bp S1 mutation
2.3
pMIR-REPORT-DANCR; 7328 bp S2 mutation
2.4
pMIR-REPORT-DANCR; 7328 bp S12 mutation
2.5
pMIR-REPORT-c-Myb 3'UTR
2.6
pMIR-REPORT-c-Myb 3' UTR 7613bp (S1 mutationc-myb 3'UTR mut-233-239)
2.7
pMIR-REPORT-c-Myb 3' UTR 7613bp (S2 mutationc-myb 3'UTR mut-1157-1164)
2.8
pMIR-REPORT-c-Myb 3' UTR 7613bp (S12 mutationc-myb 3'UTR mut-1157-1164)
3.
Empty vector
3.1
pMD2.G
3.2
psPAX2
3.3
pLKO_TR005
3.4
pMIR-REPORT luciferase
3.5
pcDNA3.1
3.6
pcDNA3.1(+) (from 14F 生醫資源中心)
4.
SEMA5A
4.1
pN1-Sema5A-ICD-18xHis
4.2
pN1-18xHis-Sema5A-ECD
4.3
pN1-Sema5A-ECD-FLAG;
5.
NDRG1
5.1
pGL3-NDRG1-OT1 promoter
5.2
pcDNA3.1(+)-NDRG1-OT1_v4
5.3
pcDNA3.1(+)-NDRG1
5.4
pGL3-basic/NDRG1 promoter -800/+353(L)
5.5
pGL3-basic/NDRG1 promoter -378/+353(S)
5.6
pGL3-basic/NDRG1 promoter -245/+353(XS)
5.7
pGL3-basic/NDRG1 promoter +21/+353(XSM)
5.8
pGL3-basic/NDRG1 promoter (M)
5.9
pGL3 OT1 promoter s1M
5.10
pGL3 OT1 promoter s2M
5.11
pGL3 OT1 promoter s1+2M
5.12
pGL3-basic vector
5.13
pMIR-REPORT/NDRG1 3'UTR
6.
MicroRNA
6.1
pcDNA6.2 miR 2276
6.2
pcDNA6.2 miR 1282
6.3
pcDNA6.2 miR 501
6.4
pcDNA6.2 miR 449a
6.5
pcDNA6.2-GW/EmGFP-miR-neg-miR-769-3p
6.6
pcDNA6.2-GW_EmGFP-miR-neg
6.7
pcDNA6.2-GW_EmGFP-hsa-miR-875-3p
6.8
pcDNA6.2-GW_EmGFP-hsa-miR-3922-5p
7.
LncRNA
7.1
pcDNA3.1-lnc-ENO2-OT1(LRRC23-2-1)
7.2
pCMV_MALAT1
7.3
pLKO_TRC005_shMALAT1 #1
7.4
pLKO_TRC005_shMALAT1 #2
7.5
pLKO_TRC005_shMALAT1 #4
7.6
pLKO_TRC005_shNDRG1-OT1 #2
7.7
pLKO_TRC005_shNDRG1-OT1 #3
7.8
pcDNA3.1(+)OT1-263bp(1-263)
7.9
pcDNA3.1(+)OT1-245bp(264-508)
7.10
pcDNA3.1(+)-OT1-149bp (1-149)
7.11
pcDNA3.1(+)-OT1-114bp (150-263)
7.12
pcDNA3.1(+)-OT1-129bp (264-392)
7.13
pcDNA3.1(+)-OT1-116bp (393-508)
7.14
pcDNA3.1 / ZEO(+) NDRG1-OT1_v4 with HA and FLAG tag -1
7.15
pcDNA3.1 / ZEO(+) NDRG1-OT1_v4 with HA and FLAG tag -2
7.16
pcDNA3.1 / ZEO(+) NDRG1-OT1_v4 with HA and FLAG tag -3
7.17
pcDNA3.1 / ZEO(+) NDRG1-OT1_v4 with HA and FLAG tag -4
7.18
pcDNA3.1-NONHSAT135851.2 (MTORT1) (peptide validation)
7.19
pcDNA3.1(+)-NONHSAT167136.1-FLAG
7.20
pcDNA3.1(+)-NONHSAT167136.1
8.
CircRNA
8.1
pCIRC2T7-mCherry
8.2
pCIRC2T7-circAAGAB
8.3
pCIRC2T7-HA-circAAGAB-Flag
8.4
pCIRC2T7-circSFMBT2
8.5
pCIRC2T7-HA-SFMBT2-FLAG
9.
Others
9.1
HA-HIF2alpha-P405A/P531A-pcDNA3(P405A/P531A mutation 用於過表現HIF-2a且在normoxia下不會被分解)
9.2
HA-HIF1alpha P402A/P564A-pcDNA3 (P402A/P564A mutation 用於過表現HIF-1a且在normoxia下不會被分解)
9.3
pcDNA3 HIF2A
9.4
IRES-Luc-NDRG1
9.5
IRES-Luc-Empty
9.6
pGFP-TCL
9.7
HA tag positive control (Western Blot:35kDa)
9.8
pcDNA 3.1(+)-FUS
9.9
pcDNA3.1(+)-hPABPC1
9.10
pcDNA3.1(+)-Alu-circSFMBT2
10.
luciferase contruct - pMIR
10.1
pmirGLO Dual Luciferase miRNATarget Expression Vector
10.2
pmirGLO _MALAT1 S1 for miR-3064
10.3
pmirGLO _MALAT1 S1mut for miR-3064
10.4
pmirGLO _MALAT1 S2 for miR-3064
10.5
pmirGLO _MALAT1 S2mut for miR-3064
10.6
pMIR-REPORT-NDRG1-OT1
10.7
pMIR-REPORT-NDRG1-OT1-mutation 1 (for miR-875-3p)
10.8
pMIR-REPORT-NDRG1-OT1-mutation 2 (for miR-875-3p)
10.9
pMIR-REPORT-NDRG1-OT1-mutation 1+2 (for miR-875-3p)
10.10
pMIR-REPORT Luc-MTORT1 (NONHSAT135851.2)
10.11
pMIR-REPORT Luc-MTORT1 mutant
10.12
pmirGLO _basic_puma from 湖孟君老師的學生賴財春
11.
MALAT1 promoter luciferase
11.1
pGL3-Basic-hMALAT1 (-2000~+24) 6777 bp
11.2
pGL3-Basic-hMALAT1 (-2000~+24) mutant 1 6777 bp
11.3
pGL3-Basic-hMALAT1 (-2000~+24) mutant 2 6777 bp
11.4
pGL3-Basic-hMALAT1 (-2000~+24) mutant 3 6777 bp
11.5
pGL3-Basic-hMALAT1 (-2000_+24) mutant 1_2_3
11.6
hsa-miR-3064 mimic
12.
pCDH-CMV-hMETTL3-EF1-Puro (over express METTL3)
