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Plasmid datasheet:
DNA sequencing:
Attachment(s):
Keypoint
  1. 1.
    Plasmid Name and Size: pN1-Sema5A-ICD-18xHis; 4336 bp
    * developed at the BioMed Resource Core of the 1st Core Facility Lab, NTU-CM
    * Sema5A-ICD: Homo sapiens semaphorin 5A-intracellular domain
    * NCBI Reference Sequence: NM_003966.2
    * The pN1 vector is modified from pEGFP-N1
    * Primers:
    8ca46957f07a2728e581e3f32b437a9b.png
    * £ TA cloning vector or R Mammalian; £Yeast; £ E. coli; expression vector
    * plasmid amplify in E. coli is R high; or £ low copy number
    * expression level is £ low or R high
    * expression promoter: CMV immediate early promoter
    * antibiotic selection: Kan and Neo
    * epitope or tag: 18xHis
     
    7d5b6f0c7ae8c87b9baebd0528f7ea4e.png
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    Location
    1. Media Center
    2. Overview
    3. 實驗室資料夾
    4. 質體清單
    Folder name
    質體清單
    Author
    賴亮全
    Branch
    賴亮全教授
    Created
    2019-06-06 14:46:25
    Last Updated
    2019-07-24 06:35:00
    Browse
    373
    1. 2.
      巧宜 質體
    2. 3.
      Empty vector
    3. 4.
      SEMA5A
    4. 5.
      NDRG1
    5. 6.
      MicroRNA
    6. 7.
      LncRNA
    7. 8.
      CircRNA
    8. 9.
      Others
    9. 10.
      luciferase contruct - pMIR
    10. 11.
      MALAT1 promoter luciferase