維持者: 陳姳蓁
存放位置: [-20C冰箱][3層][1櫃][Master box盒][64格]
Plasmid datasheet:
DNA sequencing:
存放位置: [-20C冰箱][3層][1櫃][Master box盒][64格]
Plasmid datasheet:
DNA sequencing:
Attachment(s):
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1.
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2.
Keypoint
- 1.Plasmid Name and Size: pN1-Sema5A-ICD-18xHis; 4336 bp
* developed at the BioMed Resource Core of the 1st Core Facility Lab, NTU-CM
* Sema5A-ICD: Homo sapiens semaphorin 5A-intracellular domain
* NCBI Reference Sequence: NM_003966.2
* The pN1 vector is modified from pEGFP-N1
* Primers:
* £ TA cloning vector or R Mammalian; £Yeast; £ E. coli; expression vector
* plasmid amplify in E. coli is R high; or £ low copy number
* expression level is £ low or R high
* expression promoter: CMV immediate early promoter
* antibiotic selection: Kan and Neo
* epitope or tag: 18xHis
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- Location
-
- Folder name
- 質體清單
- Author
- 賴亮全
- Branch
- 賴亮全教授
- Created
- 2019-06-06 14:46:25
- Last Updated
- 2019-07-24 06:35:00
- Browse
- 426
- 2.巧宜 質體
- 3.Empty vector
- 4.SEMA5A
- 5.NDRG1
- 6.MicroRNA
- 7.LncRNA
- 8.CircRNA
- 9.Others
- 10.luciferase contruct - pMIR
- 11.MALAT1 promoter luciferase
