維持者: 陳姳蓁
存放位置: [-20C冰箱][3層][1櫃][Master box盒][64格]
Plasmid datasheet:
DNA sequencing:
Keypoint
  1. 1.
    Plasmid Name and Size: pN1-Sema5A-ICD-18xHis; 4336 bp
    * developed at the BioMed Resource Core of the 1st Core Facility Lab, NTU-CM
    * Sema5A-ICD: Homo sapiens semaphorin 5A-intracellular domain
    * NCBI Reference Sequence: NM_003966.2
    * The pN1 vector is modified from pEGFP-N1
    * Primers:
    8ca46957f07a2728e581e3f32b437a9b.png
    * £ TA cloning vector or R Mammalian; £Yeast; £ E. coli; expression vector
    * plasmid amplify in E. coli is R high; or £ low copy number
    * expression level is £ low or R high
    * expression promoter: CMV immediate early promoter
    * antibiotic selection: Kan and Neo
    * epitope or tag: 18xHis
     
    7d5b6f0c7ae8c87b9baebd0528f7ea4e.png
    Location
    Folder name
    質體清單
    Author
    賴亮全
    Branch
    賴亮全教授
    Created
    2019-06-06 14:46:25
    Last Updated
    2019-07-24 06:35:00
    1. 2.
      巧宜 質體
    2. 3.
      Empty vector
    3. 4.
      SEMA5A
    4. 5.
      NDRG1
    5. 6.
      MicroRNA
    6. 7.
      LncRNA
    7. 8.
      CircRNA
    8. 9.
      Others
    9. 10.
      luciferase contruct - pMIR
    10. 11.
      MALAT1 promoter luciferase