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定序可使用之雞籠米克斯 免費 primer第19個RV primer3 : 5'-CTA GCA AAA TAG GCT GTC CC-3'
Plasmid datasheet:
DNA sequencing:
Keypoint
  1. 1.
    Plasmid Data Sheet
    * Plasmid Name and Size: pGL3-NDRG1-OT1 promoter; 6772 bp
    * developed at the BioMed Resource Core of the 1st Core Facility Lab, NTU-CM
    * NDRG1-OT1: a hypoxia-induced lncRNA
    * Template: Genomic DNA of HaCaT cells
    * primers:
    72013b6aae2609b365e525361d220d59.png
    * £ TA cloning vector or R Mammalian; £Yeast; £ E. coli; expression vector
    * plasmid amplify in E. coli is £ high; or R low copy number
    * promoter of Luciferase Reporter: NDRG1-OT1 promoter
    * antibiotic selection: AmpR
    * epitope or tag: non
    * NCBI Reference Sequence: NC_000008.11
    The NDRG1-OT1 promoter has 6 mutations
    afd7cf05d43db9c8ce2490e5bf9ceba6.png
    Location
    Folder name
    質體清單
    Author
    賴亮全
    Branch
    賴亮全教授
    Created
    2019-06-06 14:49:33
    Last Updated
    2019-12-13 12:00:40
    Browse
    639
    1. 2.
      巧宜 質體
    2. 3.
      Empty vector
    3. 4.
      SEMA5A
    4. 5.
      NDRG1
    5. 6.
      MicroRNA
    6. 7.
      LncRNA
    7. 8.
      CircRNA
    8. 9.
      Others
    9. 10.
      luciferase contruct - pMIR
    10. 11.
      MALAT1 promoter luciferase