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維持者: 陳姳蓁
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Plasmid datasheet:
DNA sequencing:
附件:
 
Attachment(s):
Keypoint
  1. 1.
    Plasmid Data Sheet
    * Plasmid Name and Size: pN1-Sema5A-ECD-FLAG; 6895 bp
    * developed at the BioMed Resource Core of the 1st Core Facility Lab, NTU-CM
    * Sema5A-ECD: Homo sapiens semaphorin 5A-extracellular domain
    * NCBI Reference Sequence: NM_003966.2
    * The pN1 vector is modified from pEGFP-N1
    * Primers:
    68fdc4b841f4801e03ca14e43fb83573.png
    * Mammalian expression vector
    * plasmid amplify in E. coli is high copy number
    * expression level is high
    * expression promoter: CMV immediate early promoter
    * antibiotic selection: Kan and Neo
    * epitope or tag: FLAG
    ad41eb9cbe9fb4500c3893290a32d0fa.png
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    Location
    1. Media Center
    2. Overview
    3. 實驗室資料夾
    4. 質體清單
    Folder name
    質體清單
    Author
    陳姳蓁(游薏陵)
    Branch
    賴亮全教授
    Created
    2019-12-25 15:52:04
    Last Updated
    2019-12-25 15:57:25
    Browse
    410
    1. 2.
      巧宜 質體
    2. 3.
      Empty vector
    3. 4.
      SEMA5A
    4. 5.
      NDRG1
    5. 6.
      MicroRNA
    6. 7.
      LncRNA
    7. 8.
      CircRNA
    8. 9.
      Others
    9. 10.
      luciferase contruct - pMIR
    10. 11.
      MALAT1 promoter luciferase