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  3. 實驗室資料夾
  4. 質體清單
維持者: 陳姳蓁
存放位置: [-20C冰箱][3層][1櫃][Master box盒][61格]
Plasmid datasheet:
DNA sequencing:
附件:
 
Attachment(s):
Keypoint
  1. 1.
    Plasmid Data Sheet
    * Plasmid Name and Size: pN1-Sema5A-ECD-FLAG; 6895 bp
    * developed at the BioMed Resource Core of the 1st Core Facility Lab, NTU-CM
    * Sema5A-ECD: Homo sapiens semaphorin 5A-extracellular domain
    * NCBI Reference Sequence: NM_003966.2
    * The pN1 vector is modified from pEGFP-N1
    * Primers:
    68fdc4b841f4801e03ca14e43fb83573.png
    * Mammalian expression vector
    * plasmid amplify in E. coli is high copy number
    * expression level is high
    * expression promoter: CMV immediate early promoter
    * antibiotic selection: Kan and Neo
    * epitope or tag: FLAG
    ad41eb9cbe9fb4500c3893290a32d0fa.png
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    Location
    1. Media Center
    2. Overview
    3. 實驗室資料夾
    4. 質體清單
    Folder name
    質體清單
    Author
    陳姳蓁(游薏陵)
    Branch
    賴亮全教授
    Created
    2019-12-25 15:52:04
    Last Updated
    2019-12-25 15:57:25
    1. 1.
      質體(plasmid/vector)清單
      Responsive image
    2. 2.
      巧宜 質體
      1. 2.1
        pMIR-REPORT-DANCR
      2. 2.2
        pMIR-REPORT-DANCR; 7328 bp S1 mutation
      3. 2.3
        pMIR-REPORT-DANCR; 7328 bp S2 mutation
      4. 2.4
        pMIR-REPORT-DANCR; 7328 bp S12 mutation
      5. 2.5
        pMIR-REPORT-c-Myb 3'UTR
      6. 2.6
        pMIR-REPORT-c-Myb 3' UTR 7613bp (S1 mutationc-myb 3'UTR mut-233-239)
      7. 2.7
        pMIR-REPORT-c-Myb 3' UTR 7613bp (S2 mutationc-myb 3'UTR mut-1157-1164)
      8. 2.8
        pMIR-REPORT-c-Myb 3' UTR 7613bp (S12 mutationc-myb 3'UTR mut-1157-1164)
    3. 3.
      Empty vector
      1. 3.1
        pMD2.G
      2. 3.2
        psPAX2
      3. 3.3
        pLKO_TR005
      4. 3.4
        pMIR-REPORT luciferase
      5. 3.5
        pcDNA3.1
      6. 3.6
        pcDNA3.1(+) (from 14F 生醫資源中心)
      7. 3.7
        pmirGLO Dual Luciferase miRNATarget Expression Vector
    4. 4.
      SEMA5A
      1. 4.1
        pN1-Sema5A-ICD-18xHis
      2. 4.2
        pN1-18xHis-Sema5A-ECD
      3. 4.3
        pN1-Sema5A-ECD-FLAG;
    5. 5.
      NDRG1
      1. 5.1
        pGL3-NDRG1-OT1 promoter
      2. 5.2
        pcDNA3.1(+)-NDRG1-OT1_v4
      3. 5.3
        pcDNA3.1(+)-NDRG1
      4. 5.4
        pGL3-basic/NDRG1 promoter -800/+353(L)
      5. 5.5
        pGL3-basic/NDRG1 promoter -378/+353(S)
      6. 5.6
        pGL3-basic/NDRG1 promoter -245/+353(XS)
      7. 5.7
        pGL3-basic/NDRG1 promoter +21/+353(XSM)
      8. 5.8
        pGL3-basic/NDRG1 promoter (M)
      9. 5.9
        pGL3 OT1 promoter s1M
      10. 5.10
        pGL3 OT1 promoter s2M
      11. 5.11
        pGL3 OT1 promoter s1+2M
      12. 5.12
        pGL3-basic vector
      13. 5.13
        pMIR-REPORT/NDRG1 3'UTR
    6. 6.
      MicroRNA
      1. 6.1
        pcDNA6.2 miR 2276
      2. 6.2
        pcDNA6.2 miR 1282
      3. 6.3
        pcDNA6.2 miR 501
      4. 6.4
        pcDNA6.2 miR 449a
      5. 6.5
        pcDNA6.2-GW/EmGFP-miR-neg-miR-769-3p
      6. 6.6
        pcDNA6.2-GW_EmGFP-miR-neg
      7. 6.7
        pcDNA6.2-GW_EmGFP-hsa-miR-875-3p
      8. 6.8
        pcDNA6.2-GW_EmGFP-hsa-miR-3922-5p
    7. 7.
      LncRNA
      1. 7.1
        pcDNA3.1-lnc-ENO2-OT1(LRRC23-2-1)
      2. 7.2
        pCMV_MALAT1
      3. 7.3
        pLKO_TRC005_shMALAT1 #1
      4. 7.4
        pLKO_TRC005_shMALAT1 #2
      5. 7.5
        pLKO_TRC005_shMALAT1 #4
      6. 7.6
        pLKO_TRC005_shNDRG1-OT1 #2
      7. 7.7
        pLKO_TRC005_shNDRG1-OT1 #3
      8. 7.8
        pcDNA3.1(+)OT1-263bp(1-263)
      9. 7.9
        pcDNA3.1(+)OT1-245bp(264-508)
      10. 7.10
        pcDNA3.1(+)-OT1-149bp (1-149)
      11. 7.11
        pcDNA3.1(+)-OT1-114bp (150-263)
      12. 7.12
        pcDNA3.1(+)-OT1-129bp (264-392)
      13. 7.13
        pcDNA3.1(+)-OT1-116bp (393-508)
      14. 7.14
        pcDNA3.1 / ZEO(+) NDRG1-OT1_v4 with HA and FLAG tag -1
      15. 7.15
        pcDNA3.1 / ZEO(+) NDRG1-OT1_v4 with HA and FLAG tag -2
      16. 7.16
        pcDNA3.1 / ZEO(+) NDRG1-OT1_v4 with HA and FLAG tag -3
      17. 7.17
        pcDNA3.1 / ZEO(+) NDRG1-OT1_v4 with HA and FLAG tag -4
      18. 7.18
        pcDNA3.1-NONHSAT135851.2 (MTORT1) (peptide validation)
      19. 7.19
        pcDNA3.1(+)-NONHSAT167136.1-FLAG
      20. 7.20
        pcDNA3.1(+)-NONHSAT167136.1
    8. 8.
      CircRNA
      1. 8.1
        pCIRC2T7-mCherry
      2. 8.2
        pCIRC2T7-circAAGAB
      3. 8.3
        pCIRC2T7-HA-circAAGAB-Flag
      4. 8.4
        pCIRC2T7-circSFMBT2
      5. 8.5
        pCIRC2T7-HA-SFMBT2-FLAG
      6. 8.6
        pcDNA3.1(+)-Alu-circSFMBT2
      7. 8.7
        pcDNA3.1(+)-Alu-circSFMBT2 both truncated
      8. 8.8
        pcDNA3.1(+)-Alu-circSFMBT2-AluSc5 truncated-EGFP
      9. 8.9
        pcDNA3.1(+)-Alu-circSFMBT2-AluY truncated-EGFP
      10. 8.10
        pcDNA3.1(+)-Alu-circSFMBT2-both truncated-EGFP
      11. 8.11
        pcDNA3.1(+)-Alu-circSFMBT2-EGFP
      12. 8.12
        pcDNA3.1(+)-circACVR2A
        1. 8.12.1
          pcDNA3.1(+)-circACVR2A
    9. 9.
      Others
      1. 9.1
        HA-HIF2alpha-P405A/P531A-pcDNA3(P405A/P531A mutation 用於過表現HIF-2a且在normoxia下不會被分解)
      2. 9.2
        HA-HIF1alpha P402A/P564A-pcDNA3 (P402A/P564A mutation 用於過表現HIF-1a且在normoxia下不會被分解)
      3. 9.3
        pcDNA3 HIF2A
      4. 9.4
        IRES-Luc-NDRG1
      5. 9.5
        IRES-Luc-Empty
      6. 9.6
        pGFP-TCL
      7. 9.7
        HA tag positive control (Western Blot:35kDa)
      8. 9.8
        pcDNA 3.1(+)-FUS
      9. 9.9
        pcDNA3.1(+)-hPABPC1
      10. 9.10
        pCDH-CMV-MYC-EF1-Puro
    10. 10.
      luciferase contruct - pMIR
      1. 10.1
        pmirGLO Dual Luciferase miRNATarget Expression Vector
      2. 10.2
        pmirGLO _MALAT1 S1 for miR-3064
      3. 10.3
        pmirGLO _MALAT1 S1mut for miR-3064
      4. 10.4
        pmirGLO _MALAT1 S2 for miR-3064
      5. 10.5
        pmirGLO _MALAT1 S2mut for miR-3064
      6. 10.6
        pMIR-REPORT-NDRG1-OT1
      7. 10.7
        pMIR-REPORT-NDRG1-OT1-mutation 1 (for miR-875-3p)
      8. 10.8
        pMIR-REPORT-NDRG1-OT1-mutation 2 (for miR-875-3p)
      9. 10.9
        pMIR-REPORT-NDRG1-OT1-mutation 1+2 (for miR-875-3p)
      10. 10.10
        pMIR-REPORT Luc-MTORT1 (NONHSAT135851.2)
      11. 10.11
        pMIR-REPORT Luc-MTORT1 mutant
      12. 10.12
        pmirGLO _basic_puma from 湖孟君老師的學生賴財春
      13. 10.13
        pmirGLO-SFMBT2-HRE
      14. 10.14
        pmirGLO-SFMBT2-HRE1 mutant
      15. 10.15
        pmirGLO-SFMBT2-HRE3 mutant
      16. 10.16
        pmirGLO-SFMBT2-HRE1-HRE3 mutant
    11. 11.
      MALAT1 promoter luciferase
      1. 11.1
        pGL3-Basic-hMALAT1 (-2000~+24) 6777 bp
      2. 11.2
        pGL3-Basic-hMALAT1 (-2000~+24) mutant 1 6777 bp
      3. 11.3
        pGL3-Basic-hMALAT1 (-2000~+24) mutant 2 6777 bp
      4. 11.4
        pGL3-Basic-hMALAT1 (-2000~+24) mutant 3 6777 bp
      5. 11.5
        pGL3-Basic-hMALAT1 (-2000_+24) mutant 1_2_3
      6. 11.6
        hsa-miR-3064 mimic
    12. 12.
      pCDH-CMV-hMETTL3-EF1-Puro (over express METTL3)

    臺北市仁愛路一段1號10樓1009室

    R1009 10F No1 Sec1 Ren-Ai Rd, Taipei 100233, Taiwan.

    E-mail: llai@ntu.edu.tw

    Tel: 886-2-2312-3456 # 288241

    Lab: 886-2-2393-8235