• 00:25 1.
    index 1
  • 00:19 2.
    Question
  • 03:27 3.
    Sample Acquisition and Nucleic Acid Extraction
  • 01:37 4.
    QuestionThere were 50 µL RNA samples. You added 2 µL RNA sample to 78 µL sterile water and measured the optical density of your sample at a UV wavelength of 260 nm. If the  absorbance reading was 0.08, what was the initial amount of your RNA sample?6.4 µg
  • 02:07 5.
    Methods to Check NA Quality1
  • 00:27 6.
    QuestionWhen you check the integrity of mRNA, why do you check the integrity of ribosomal RNA (28S and 18S rRNA)?
  • 02:00 7.
    Methods to Check NA Quality2
  • 02:49 8.
    RIN－RNA Integrity Number
  • 03:29 9.
    Methods to Check NA Quality3
  • 01:17 10.
    Methods to Check RNA Quality4
  • 00:37 11.
    Steps for Microarray Experiment
  • 00:10 12.
    Sample Amplification / Labeling
  • 00:58 13.
    In Vitro Transcription
  • 00:19 14.
    Steps for Microarray Experiment
  • 00:15 15.
    Hybridization & Scanning
  • 00:54 16.
    Steps for Microarray Experiment
  • 01:43 17.
    Visual inspection of array features
  • 02:48 18.
    Box plots consist of boxes with a central line and two tailsCentral line: median of the dataUpper (seventy-fifth percentile, Q3) and lower quartile (twenty-fifth percentile, Q1) Ends of the whiskers:1.5x IQR (interquartile range) Max or min value within 1
  • 01:39 19.
    Examples of Bad Slide Quality
  • 01:16 20.
    QuestionWhat are the advantages of using Illumina bead arrays?
  • 01:22 21.
    Steps for Microarray Experiment
  • 03:04 22.
    Normalization1
  • 02:48 23.
    Normalization2
  • 04:19 24.
    Normalization3
  • 01:15 25.
    Single Channel Normalization and Scaling
  • 01:33 26.
    Summarization
  • 01:26 27.
    Visualization Tools for Microarray Analysis
  • 01:30 28.
    Heat Map and Dendrogram
  • 01:11 29.
    Violin Plot
  • 02:09 30.
    Scatter Plot
  • 00:23 31.
    MA Plot
  • 04:33 32.
    Volcano plot
  • 03:49 33.
    Bar Chart
  • 02:36 34.
    Line Graph
  • 00:58 35.
    Pie Chart
  • 01:16 36.
    Venn Diagram
  • 00:51 37.
    Starburst Plot
  • 02:47 38.
    Visualization of Gene Distribution
  • 03:19 39.
    Visualization of Gene Distribution
  • 02:29 40.
    PCA and MDS
  • 02:36 41.
    MDS (Multidimensional Scaling)Principal Coordinate Analysis (PCoA)
  • 00:21 42.
    Correspondence Analysis (CA)
  • 00:55 43.
    QuestionIf you want to check data quality of microarray experiments, which graph will not be used? Scatter plot of PCAHeat mapBox plotVenn diagram
  • 00:09 44.
    Steps for Microarray Experiment
  • 03:26 45.
    Precision vs Accuracy
  • 06:28 46.
    Confusion Matrix (混淆矩陣)
  • 01:12 47.
    Sensitivity vs Specificity
  • 01:08 48.
    Positive Predictive Value (PPV) vs Negative Predictive Value (NPV)
  • 00:31 49.
    Precision (PPV) vs Recall (Sensitivity)
  • 02:28 50.
    Accuracy Paradox
  • 01:29 51.
    F1 Score
  • 01:26 52.
    Alpha vs Beta value
  • 02:42 53.
    Power as a Function of Alpha and Sample Size
  • 01:09 54.
    Sample Size Calculation
  • 01:02 55.
    How to Decide the Cutpoint?
  • 03:15 56.
    Contradiction Between Sensitivity & Specificity
  • 02:07 57.
    Receiver Operating CharacteristicCurve
  • 00:34 58.
    Receiver Operating CharacteristicCurve
  • 01:54 59.
    Summary of Statistics
  • Index
  • Notes
  • Comment
  • Fullscreen
Comparative Gene-Expression Analysis I: Hybridization-based techniques (0309)
Duration: 1:47:36, Browse: 389, Last Updated: 2021-03-10
    Prev Next
    • 00:25 1.
      index 1
    • 00:19 2.
      Question
    • 03:27 3.
      Sample Acquisition and Nucleic Acid Extraction
    • 01:37 4.
      QuestionThere were 50 µL RNA samples. You added 2 µL RNA sample to 78 µL sterile water and measured the optical density of your sample at a UV wavelength of 260 nm. If the  absorbance reading was 0.08, what was the initial amount of your RNA sample?6.4 µg
    • 02:07 5.
      Methods to Check NA Quality1
    • 00:27 6.
      QuestionWhen you check the integrity of mRNA, why do you check the integrity of ribosomal RNA (28S and 18S rRNA)?
    • 02:00 7.
      Methods to Check NA Quality2
    • 02:49 8.
      RIN－RNA Integrity Number
    • 03:29 9.
      Methods to Check NA Quality3
    • 01:17 10.
      Methods to Check RNA Quality4
    • 00:37 11.
      Steps for Microarray Experiment
    • 00:10 12.
      Sample Amplification / Labeling
    • 00:58 13.
      In Vitro Transcription
    • 00:19 14.
      Steps for Microarray Experiment
    • 00:15 15.
      Hybridization & Scanning
    • 00:54 16.
      Steps for Microarray Experiment
    • 01:43 17.
      Visual inspection of array features
    • 02:48 18.
      Box plots consist of boxes with a central line and two tailsCentral line: median of the dataUpper (seventy-fifth percentile, Q3) and lower quartile (twenty-fifth percentile, Q1) Ends of the whiskers:1.5x IQR (interquartile range) Max or min value within 1
    • 01:39 19.
      Examples of Bad Slide Quality
    • 01:16 20.
      QuestionWhat are the advantages of using Illumina bead arrays?
    • 01:22 21.
      Steps for Microarray Experiment
    • 03:04 22.
      Normalization1
    • 02:48 23.
      Normalization2
    • 04:19 24.
      Normalization3
    • 01:15 25.
      Single Channel Normalization and Scaling
    • 01:33 26.
      Summarization
    • 01:26 27.
      Visualization Tools for Microarray Analysis
    • 01:30 28.
      Heat Map and Dendrogram
    • 01:11 29.
      Violin Plot
    • 02:09 30.
      Scatter Plot
    • 00:23 31.
      MA Plot
    • 04:33 32.
      Volcano plot
    • 03:49 33.
      Bar Chart
    • 02:36 34.
      Line Graph
    • 00:58 35.
      Pie Chart
    • 01:16 36.
      Venn Diagram
    • 00:51 37.
      Starburst Plot
    • 02:47 38.
      Visualization of Gene Distribution
    • 03:19 39.
      Visualization of Gene Distribution
    • 02:29 40.
      PCA and MDS
    • 02:36 41.
      MDS (Multidimensional Scaling)Principal Coordinate Analysis (PCoA)
    • 00:21 42.
      Correspondence Analysis (CA)
    • 00:55 43.
      QuestionIf you want to check data quality of microarray experiments, which graph will not be used? Scatter plot of PCAHeat mapBox plotVenn diagram
    • 00:09 44.
      Steps for Microarray Experiment
    • 03:26 45.
      Precision vs Accuracy
    • 06:28 46.
      Confusion Matrix (混淆矩陣)
    • 01:12 47.
      Sensitivity vs Specificity
    • 01:08 48.
      Positive Predictive Value (PPV) vs Negative Predictive Value (NPV)
    • 00:31 49.
      Precision (PPV) vs Recall (Sensitivity)
    • 02:28 50.
      Accuracy Paradox
    • 01:29 51.
      F1 Score
    • 01:26 52.
      Alpha vs Beta value
    • 02:42 53.
      Power as a Function of Alpha and Sample Size
    • 01:09 54.
      Sample Size Calculation
    • 01:02 55.
      How to Decide the Cutpoint?
    • 03:15 56.
      Contradiction Between Sensitivity & Specificity
    • 02:07 57.
      Receiver Operating CharacteristicCurve
    • 00:34 58.
      Receiver Operating CharacteristicCurve
    • 01:54 59.
      Summary of Statistics
    Location
    1. Media Center
    2. ...
    3. 2021
    Folder name
    2021
    Author
    賴亮全
    Branch
    賴亮全教授
    Created
    2021-03-09 16:21:10
    Last Updated
    2021-03-10 13:43:59
    Browse
    389
    Duration
    1:47:36