• 00:00 1.
    Effects of Efficiency
  • 01:49 2.
    Melting Curve
  • 03:27 3.
    Melting Curve
  • 04:23 4.
    Relative Quantity Value: 2-ΔΔCT
  • 09:57 5.
    Question:Given the following Ct values, please calculate the fold change of YFG in experiment group.
  • 10:44 6.
    Real Time - PCR
  • 11:57 7.
    SYBR Green Dye Assay
  • 12:40 8.
    Fluorescence Resonance Energy Transfer (FRET)
  • 13:58 9.
    Principle of TaqMan Assay
  • 16:33 10.
    TaqMan RT-PCR assay
  • 18:48 11.
    LightCycler Probes
  • 19:51 12.
    Molecular Beacons
  • 20:30 13.
    QuestionHow to amplify strand-specific DNA by PCR?
  • 21:07 14.
    Strand Specific PCR
  • 24:45 15.
    Absolute vs. Relative Quantification for qPCR
  • 25:51 16.
    QuestionWhat is the difference between analog vs digital signal?
  • 26:42 17.
    Digital PCR
  • 31:46 18.
    Digital PCR
  • Index
  • Notes
  • Comment
  • Fullscreen
Comparative Gene-Expression Analysis II: PCR-based techniques (0330)
Duration: 33:51, Browse: 444, Last Updated: 2021-03-30
    • 00:00 1.
      Effects of Efficiency
    • 01:49 2.
      Melting Curve
    • 03:27 3.
      Melting Curve
    • 04:23 4.
      Relative Quantity Value: 2-ΔΔCT
    • 09:57 5.
      Question:Given the following Ct values, please calculate the fold change of YFG in experiment group.
    • 10:44 6.
      Real Time - PCR
    • 11:57 7.
      SYBR Green Dye Assay
    • 12:40 8.
      Fluorescence Resonance Energy Transfer (FRET)
    • 13:58 9.
      Principle of TaqMan Assay
    • 16:33 10.
      TaqMan RT-PCR assay
    • 18:48 11.
      LightCycler Probes
    • 19:51 12.
      Molecular Beacons
    • 20:30 13.
      QuestionHow to amplify strand-specific DNA by PCR?
    • 21:07 14.
      Strand Specific PCR
    • 24:45 15.
      Absolute vs. Relative Quantification for qPCR
    • 25:51 16.
      QuestionWhat is the difference between analog vs digital signal?
    • 26:42 17.
      Digital PCR
    • 31:46 18.
      Digital PCR
    Location
    Folder name
    2021
    Author
    賴亮全
    Branch
    賴亮全教授
    Created
    2021-03-30 16:17:46
    Last Updated
    2021-03-30 20:38:33
    Duration
    33:51