• 01:49 1.
    Effects of Efficiency
  • 01:38 2.
    Melting Curve
  • 00:55 3.
    Melting Curve
  • 05:34 4.
    Relative Quantity Value: 2-ΔΔCT
  • 00:46 5.
    Question:Given the following Ct values, please calculate the fold change of YFG in experiment group.
  • 01:12 6.
    Real Time - PCR
  • 00:43 7.
    SYBR Green Dye Assay
  • 01:17 8.
    Fluorescence Resonance Energy Transfer (FRET)
  • 02:35 9.
    Principle of TaqMan Assay
  • 02:14 10.
    TaqMan RT-PCR assay
  • 01:02 11.
    LightCycler Probes
  • 00:39 12.
    Molecular Beacons
  • 00:37 13.
    QuestionHow to amplify strand-specific DNA by PCR?
  • 03:37 14.
    Strand Specific PCR
  • 01:06 15.
    Absolute vs. Relative Quantification for qPCR
  • 00:50 16.
    QuestionWhat is the difference between analog vs digital signal?
  • 05:03 17.
    Digital PCR
  • 02:04 18.
    Digital PCR
  • Index
  • Notes
  • Comment
  • Fullscreen
Comparative Gene-Expression Analysis II: PCR-based techniques (0330)
Duration: 33:51, Browse: 301, Last Updated: 2021-03-30
    • 01:49 1.
      Effects of Efficiency
    • 01:38 2.
      Melting Curve
    • 00:55 3.
      Melting Curve
    • 05:34 4.
      Relative Quantity Value: 2-ΔΔCT
    • 00:46 5.
      Question:Given the following Ct values, please calculate the fold change of YFG in experiment group.
    • 01:12 6.
      Real Time - PCR
    • 00:43 7.
      SYBR Green Dye Assay
    • 01:17 8.
      Fluorescence Resonance Energy Transfer (FRET)
    • 02:35 9.
      Principle of TaqMan Assay
    • 02:14 10.
      TaqMan RT-PCR assay
    • 01:02 11.
      LightCycler Probes
    • 00:39 12.
      Molecular Beacons
    • 00:37 13.
      QuestionHow to amplify strand-specific DNA by PCR?
    • 03:37 14.
      Strand Specific PCR
    • 01:06 15.
      Absolute vs. Relative Quantification for qPCR
    • 00:50 16.
      QuestionWhat is the difference between analog vs digital signal?
    • 05:03 17.
      Digital PCR
    • 02:04 18.
      Digital PCR
    Location
    Folder name
    2021
    Author
    賴亮全
    Branch
    賴亮全教授
    Created
    2021-03-30 16:17:46
    Last Updated
    2021-03-30 20:38:33
    Browse
    301
    Duration
    33:51