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Index
00:00
1.
Effects of Efficiency
01:49
2.
Melting Curve
03:27
3.
Melting Curve
04:23
4.
Relative Quantity Value: 2-ΔΔCT
09:57
5.
Question:Given the following Ct values, please calculate the fold change of YFG in experiment group.
10:44
6.
Real Time - PCR
11:57
7.
SYBR Green Dye Assay
12:40
8.
Fluorescence Resonance Energy Transfer (FRET)
13:58
9.
Principle of TaqMan Assay
16:33
10.
TaqMan RT-PCR assay
18:48
11.
LightCycler Probes
19:51
12.
Molecular Beacons
20:30
13.
QuestionHow to amplify strand-specific DNA by PCR?
21:07
14.
Strand Specific PCR
24:45
15.
Absolute vs. Relative Quantification for qPCR
25:51
16.
QuestionWhat is the difference between analog vs digital signal?
26:42
17.
Digital PCR
31:46
18.
Digital PCR
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00:00
1.
Effects of Efficiency
01:49
2.
Melting Curve
03:27
3.
Melting Curve
04:23
4.
Relative Quantity Value: 2-ΔΔCT
09:57
5.
Question:Given the following Ct values, please calculate the fold change of YFG in experiment group.
10:44
6.
Real Time - PCR
11:57
7.
SYBR Green Dye Assay
12:40
8.
Fluorescence Resonance Energy Transfer (FRET)
13:58
9.
Principle of TaqMan Assay
16:33
10.
TaqMan RT-PCR assay
18:48
11.
LightCycler Probes
19:51
12.
Molecular Beacons
20:30
13.
QuestionHow to amplify strand-specific DNA by PCR?
21:07
14.
Strand Specific PCR
24:45
15.
Absolute vs. Relative Quantification for qPCR
25:51
16.
QuestionWhat is the difference between analog vs digital signal?
26:42
17.
Digital PCR
31:46
18.
Digital PCR
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Print date : 2025/02/13
賴亮全實驗室
Media Center
...
2021
Comparative Gene-Expression Analysis II: PCR-based techniques (0330)
Duration: 33:51,
Browse: 699,
Last Updated: 2021-03-30
Play Video: http://llai.cm.ntu.edu.tw/media/1754
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Index
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Details
00:00
1.
Effects of Efficiency
01:49
2.
Melting Curve
03:27
3.
Melting Curve
04:23
4.
Relative Quantity Value: 2-ΔΔCT
09:57
5.
Question:Given the following Ct values, please calculate the fold change of YFG in experiment group.
10:44
6.
Real Time - PCR
11:57
7.
SYBR Green Dye Assay
12:40
8.
Fluorescence Resonance Energy Transfer (FRET)
13:58
9.
Principle of TaqMan Assay
16:33
10.
TaqMan RT-PCR assay
18:48
11.
LightCycler Probes
19:51
12.
Molecular Beacons
20:30
13.
QuestionHow to amplify strand-specific DNA by PCR?
21:07
14.
Strand Specific PCR
24:45
15.
Absolute vs. Relative Quantification for qPCR
25:51
16.
QuestionWhat is the difference between analog vs digital signal?
26:42
17.
Digital PCR
31:46
18.
Digital PCR
Location
Media Center
...
2021
Folder name
2021
Author
賴亮全
Branch
賴亮全教授
Created
2021-03-30 16:17:46
Last Updated
2021-03-30 20:38:33
Duration
33:51
Media Center
...
2021
1.
Comparative Gene-Expression Analysis I: Hybridization-based techniques
1.1
Comparative Gene-Expression Analysis I: Hybridization-based techniques (0223)
1.2
Comparative Gene-Expression Analysis I: Hybridization-based techniques (0302)
1.3
Comparative Gene-Expression Analysis I: Hybridization-based techniques (0309)
1.4
Comparative Gene-Expression Analysis I: Hybridization-based techniques (0316)
1.5
Comparative Gene-Expression Analysis I: Hybridization-based techniques (0323)
2.
Comparative Gene-Expression Analysis II: PCR-based techniques
2.1
Comparative Gene-Expression Analysis II: PCR-based techniques (0323)
2.2
Comparative Gene-Expression Analysis II: PCR-based techniques (0330)
3.
Comparative Gene-Expression Analysis III: Sequence-based techniques
3.1
Comparative Gene-Expression Analysis III Sequence-based techniques (0330)
3.2
Comparative Gene-Expression Analysis III Sequence-based techniques (0413)
3.3
Comparative Gene-Expression Analysis III Sequence-based techniques (0420)
4.
DNA structure variation
4.1
Genomic approaches to study DNA structure variation (0427)
4.2
Genomic approaches to study DNA structure variation (0504)
4.3
Genomic approaches to study DNA structure variation (0511)
5.
Epigenetics
5.1
Epigenetics_nucleic acid methylation (0511)
5.2
Epigenetics_histone modification (0511)
5.3
Epigenetics_noncoding RNA (0518)
6.
Protein interaction (0525)
7.
Metagenomics (0601)
8.
Application in Cancer treatment (0608)