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02:24
1.
Other Sequencing Technology
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03:31
2.
BioNano Genomics (Irys System)
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02:33
3.
Overview of RNA-seq Library Construction
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03:34
4.
RNA Sample Preparation
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02:19
5.
RNA Quality Assessment: Agilent 2100 BioAnalyzer (RIN)RNA Quantification:The UV-absorbance method uses a spectrophotometer to measure the natural absorbance of light at 260 nm (for DNA and RNA) or 280 nm (for proteins)(A260 - A320) x RNA extinction coeffi
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01:29
6.
Input : 10 pg – 10 ng total RNA (RIN>8)
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02:08
7.
QC / Demultiplexing
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03:16
8.
Coverage
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03:37
9.
NGS Experimental Considerations
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01:39
10.
Phred - Quality Base Calling
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02:06
11.
Phred Qualities
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04:21
12.
N50 / L50
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03:04
13.
Quality Control
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02:31
14.
Quality Control – FastQC Tool for Quality Visualization
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01:30
15.
Quality Control – FastQC Tool for Quality Visualization
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00:20
16.
Quality Control – FastQC Tool for Quality Visualization
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00:26
17.
Quality Control – FastQC Tool for Quality Visualization
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00:37
18.
Quality Control – FastQC Tool for Quality Visualization
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01:02
19.
PCR Duplicate
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00:55
20.
Adapter Removal
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01:02
21.
Example
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01:32
22.
QC / Demultiplexing
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01:13
23.
Differences Between RNA-Seq and DNA-Seq
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00:55
24.
Alignment
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00:18
25.
The Seed-Filter-Extend Approach
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02:26
26.
Hash-Based Alignment Methods
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05:22
27.
Burrows–Wheeler Transform
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02:16
28.
Assembly
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02:06
29.
Problems in DNA Sequencing
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00:43
30.
Assemble algorithms
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01:58
31.
de Bruijn Graph Approach
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00:35
32.
de Bruijn Graph Approach
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01:05
33.
QC / Demultiplexing
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02:33
34.
RNA-Seq Data Normalization Methods
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00:14
35.
RPKM
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00:15
36.
Question
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06:26
37.
Drawbacks of R/FPKM
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01:18
38.
Evaluation of Normalization Methods
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00:30
39.
Workflow for Analysis of RNA-Seq Data
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01:24
40.
Tuxedo Pipeline
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00:37
41.
Strategies for Reconstructing Transcripts from RNA-Seq Reads
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00:56
42.
Differential Analysis
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02:02
43.
Improving the Human Reference Genome
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01:44
44.
Single Cell Database
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01:41
45.
Single Sample GSEA (ssGSEA)
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03:37
46.
GSVA Algorithm
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05:33
47.
GSVA Algorithm
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00:04
48.
Tools for NGS Data Analysis
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00:04
49.
ONLINE ANALYSIS TOOLShttp://molbiol-tools.ca/
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00:13
50.
Online Bioinformatics Resources Collection (OBRC)
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00:15
51.
Tools for Visualizing Short Read Data
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00:24
52.
Summary
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01:18
53.
Microarrays vs RNA-seq
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00:47
54.
Application of Sequence Census Assays