• 00:00 1.
    Multiplexed sequencing process
  • 01:26 2.
    Paired-End Sequence
  • 02:13 3.
    Mate Paired Analysis
  • 04:27 4.
    Mate-Paired Library
  • 06:39 5.
    Usages of Paired-end / Mate-paired Reads
  • 09:58 6.
    Illumina System: Low-Mid Throughput
  • 11:34 7.
    Other Sequencing Technology
  • 12:18 8.
    Complete Genomics
  • 17:47 9.
    Complete Genomics: building DNA nanoballs
  • 24:02 10.
    Complete Genomics: arraying
  • 25:32 11.
    Complete Genomics: sequencing by ligation
  • 30:57 12.
    Complete Genomics: data analysis
  • 31:09 13.
    Other Sequencing Technology
  • 31:28 14.
    Nanopore Sequencing
  • 33:23 15.
    Nanopore Sequencing
  • 35:53 16.
    Oxford Nanopore Sequencing
  • 37:52 17.
    Oxford Nanopore Sequencing
  • 39:28 18.
    Oxford Nanopore Sequencing
  • 40:08 19.
    Oxford nanopore minion video
  • 43:14 20.
    Question: how to decrease sequencing error by using nanopore sequencing?
  • 43:42 21.
    NABsys: Hybridization-Assisted Nanopore Sequencing Platform
  • 46:33 22.
    NABsys: Hybridization-Assisted Nanopore Sequencing Platform
  • 47:32 23.
    NABsys: Hybridization-Assisted Nanopore Sequencing Platform
  • 48:51 24.
    NABsys: Hybridization-Assisted Nanopore Sequencing Platform
  • 49:34 25.
    Sequencing by Expansion
  • 50:53 26.
    Sequencing by Expansion
  • 51:55 27.
    Sequencing by Expansion
  • 52:23 28.
    Sequencing by Expansion
  • 52:58 29.
    Other Sequencing Technology
  • 55:18 30.
    Pacific Bioscience
  • 56:01 31.
    Zero-mode Waveguide
  • 58:42 32.
    Zero-mode Waveguide
  • 1:00:18 33.
    Reaction Time for Single-molecule DNA Polymerase Activity
  • 1:01:29 34.
    Pacific Bioscience
  • 1:02:48 35.
    Pacific Bioscience
  • 1:04:57 36.
    PacBio Sequencing
  • 1:07:10 37.
    PacBio Sequence Generation
  • 1:08:37 38.
    Other Sequencing Technology
  • 1:09:00 39.
    Question?
  • 1:12:08 40.
    Semiconductor Sequencing:Ion Torrent
  • 1:14:13 41.
    Semiconductor Sequencing:Ion Torrent
  • 1:15:36 42.
    Semiconductor Sequencing
  • 1:16:32 43.
    Semiconductor Sequencing
  • 1:17:01 44.
    Other Sequencing Technology
  • 1:17:33 45.
    Synthetic Long-Read Sequencing10x Genomics
  • 1:18:33 46.
    Synthetic Long-Read Sequencing10x Genomics
  • 1:20:53 47.
    Linked reads: fragments sharing a same barcode that are derived from a single long moleculeEach long fragment can be stacked, combining their individual coverage into an overall map
  • 1:22:22 48.
    Various capture methods
  • 1:23:34 49.
    Single Cell RNA Sequencing
  • 1:25:52 50.
    Single Cell RNA Sequencing
  • 1:29:31 51.
    Unique Molecular Identifiers (UMI)
  • 1:31:10 52.
    Single Cell RNA Sequencing
  • 1:34:24 53.
    Comparison of Various Long-Read Methods
  • 1:35:32 54.
    Pros & Cons of Long-Read Platforms
  • 1:36:44 55.
    Cite Seq
  • Index
  • Notes
  • Comment
  • Fullscreen
Comparative Gene-Expression Analysis III Sequence-based techniques (0413)
Duration: 1:39:23, Browse: 472, Last Updated: 2021-04-21
    • 00:00 1.
      Multiplexed sequencing process
    • 01:26 2.
      Paired-End Sequence
    • 02:13 3.
      Mate Paired Analysis
    • 04:27 4.
      Mate-Paired Library
    • 06:39 5.
      Usages of Paired-end / Mate-paired Reads
    • 09:58 6.
      Illumina System: Low-Mid Throughput
    • 11:34 7.
      Other Sequencing Technology
    • 12:18 8.
      Complete Genomics
    • 17:47 9.
      Complete Genomics: building DNA nanoballs
    • 24:02 10.
      Complete Genomics: arraying
    • 25:32 11.
      Complete Genomics: sequencing by ligation
    • 30:57 12.
      Complete Genomics: data analysis
    • 31:09 13.
      Other Sequencing Technology
    • 31:28 14.
      Nanopore Sequencing
    • 33:23 15.
      Nanopore Sequencing
    • 35:53 16.
      Oxford Nanopore Sequencing
    • 37:52 17.
      Oxford Nanopore Sequencing
    • 39:28 18.
      Oxford Nanopore Sequencing
    • 40:08 19.
      Oxford nanopore minion video
    • 43:14 20.
      Question: how to decrease sequencing error by using nanopore sequencing?
    • 43:42 21.
      NABsys: Hybridization-Assisted Nanopore Sequencing Platform
    • 46:33 22.
      NABsys: Hybridization-Assisted Nanopore Sequencing Platform
    • 47:32 23.
      NABsys: Hybridization-Assisted Nanopore Sequencing Platform
    • 48:51 24.
      NABsys: Hybridization-Assisted Nanopore Sequencing Platform
    • 49:34 25.
      Sequencing by Expansion
    • 50:53 26.
      Sequencing by Expansion
    • 51:55 27.
      Sequencing by Expansion
    • 52:23 28.
      Sequencing by Expansion
    • 52:58 29.
      Other Sequencing Technology
    • 55:18 30.
      Pacific Bioscience
    • 56:01 31.
      Zero-mode Waveguide
    • 58:42 32.
      Zero-mode Waveguide
    • 1:00:18 33.
      Reaction Time for Single-molecule DNA Polymerase Activity
    • 1:01:29 34.
      Pacific Bioscience
    • 1:02:48 35.
      Pacific Bioscience
    • 1:04:57 36.
      PacBio Sequencing
    • 1:07:10 37.
      PacBio Sequence Generation
    • 1:08:37 38.
      Other Sequencing Technology
    • 1:09:00 39.
      Question?
    • 1:12:08 40.
      Semiconductor Sequencing:Ion Torrent
    • 1:14:13 41.
      Semiconductor Sequencing:Ion Torrent
    • 1:15:36 42.
      Semiconductor Sequencing
    • 1:16:32 43.
      Semiconductor Sequencing
    • 1:17:01 44.
      Other Sequencing Technology
    • 1:17:33 45.
      Synthetic Long-Read Sequencing10x Genomics
    • 1:18:33 46.
      Synthetic Long-Read Sequencing10x Genomics
    • 1:20:53 47.
      Linked reads: fragments sharing a same barcode that are derived from a single long moleculeEach long fragment can be stacked, combining their individual coverage into an overall map
    • 1:22:22 48.
      Various capture methods
    • 1:23:34 49.
      Single Cell RNA Sequencing
    • 1:25:52 50.
      Single Cell RNA Sequencing
    • 1:29:31 51.
      Unique Molecular Identifiers (UMI)
    • 1:31:10 52.
      Single Cell RNA Sequencing
    • 1:34:24 53.
      Comparison of Various Long-Read Methods
    • 1:35:32 54.
      Pros & Cons of Long-Read Platforms
    • 1:36:44 55.
      Cite Seq
    Location
    Folder name
    2021
    Author
    賴亮全
    Branch
    賴亮全教授
    Created
    2021-04-13 16:27:49
    Last Updated
    2021-04-21 09:50:45
    Duration
    1:39:23