• 00:00 1.
    Nomenclature of Cytogenetics
  • 02:57 2.
    Example of AML
  • 04:36 3.
    Question
  • 06:23 4.
    3-D Structure of Genomes: 3C
  • 07:47 5.
    3-D Structure of Genomes: 4C/5C
  • 09:11 6.
    3-D Structure of Genomes: HiC
  • 10:58 7.
    3-D Structure of Genomes: ChIA-PET
  • 12:40 8.
    Cell Immortalization
  • 16:12 9.
    Question
  • 17:13 10.
    Ways to Generate Site Specific Double-Strand DNA Breaks
  • 18:31 11.
    TALEN Technology
  • 23:39 12.
    CRISPR Genome Engineering
  • 31:21 13.
    Websites for Designing sgRNA
  • 31:28 14.
    CRISPR Genome Engineering
  • 33:47 15.
    CRISPR application
  • 35:21 16.
    Transposons
  • 37:15 17.
    PiggyBac Transposon System
  • 38:38 18.
    RNA-guided DNA transposition
  • 40:09 19.
    RNA-Guided Platform for Sequence-Specific Control of Gene
  • 43:25 20.
    Type VI CRISPR
  • 43:37 21.
    Knockout Mice vs Transgenics
  • 45:26 22.
    Making Knockout / Transgenic Mice
  • 47:02 23.
    Schematic and Time Line
  • 47:37 24.
    DNA Incorporation & Identification of Transgenic Cells
  • 48:32 25.
    Positive and Negative Selection
  • 51:19 26.
    Schematic and Time Line
  • 52:05 27.
    Schematic and Time Line
  • 54:41 28.
    Comparison of Time line
  • 57:14 29.
    Conditional Knock-out
  • 1:00:49 30.
    Tissue-Specific Knock-out
  • 1:05:04 31.
    Conditional Knock-out(Time Specific)
  • 1:07:27 32.
    Mouse Strain Nomenclature
  • 1:09:51 33.
    Transgenic Mouse Strain Nomenclature
  • 1:10:40 34.
    A Laboratory Code
  • 1:11:27 35.
    Congenic Strain
  • 1:12:21 36.
    Knockout Mouse Strain Nomenclature
  • 1:14:20 37.
    Nude mice
  • 1:18:48 38.
    Severe Combined Immunodeficiency (SCID) Mice
  • 1:20:16 39.
    NSG Mouse
  • 1:23:15 40.
    NSG-SGM3 Mouse
  • 1:24:53 41.
    Humanize PDX mouse (擬人鼠)
  • Index
  • Notes
  • Comment
  • Fullscreen
Genomic approaches to study DNA structure variation (0504)
Duration: 1:26:00, Browse: 455, Last Updated: 2021-05-05
    • 00:00 1.
      Nomenclature of Cytogenetics
    • 02:57 2.
      Example of AML
    • 04:36 3.
      Question
    • 06:23 4.
      3-D Structure of Genomes: 3C
    • 07:47 5.
      3-D Structure of Genomes: 4C/5C
    • 09:11 6.
      3-D Structure of Genomes: HiC
    • 10:58 7.
      3-D Structure of Genomes: ChIA-PET
    • 12:40 8.
      Cell Immortalization
    • 16:12 9.
      Question
    • 17:13 10.
      Ways to Generate Site Specific Double-Strand DNA Breaks
    • 18:31 11.
      TALEN Technology
    • 23:39 12.
      CRISPR Genome Engineering
    • 31:21 13.
      Websites for Designing sgRNA
    • 31:28 14.
      CRISPR Genome Engineering
    • 33:47 15.
      CRISPR application
    • 35:21 16.
      Transposons
    • 37:15 17.
      PiggyBac Transposon System
    • 38:38 18.
      RNA-guided DNA transposition
    • 40:09 19.
      RNA-Guided Platform for Sequence-Specific Control of Gene
    • 43:25 20.
      Type VI CRISPR
    • 43:37 21.
      Knockout Mice vs Transgenics
    • 45:26 22.
      Making Knockout / Transgenic Mice
    • 47:02 23.
      Schematic and Time Line
    • 47:37 24.
      DNA Incorporation & Identification of Transgenic Cells
    • 48:32 25.
      Positive and Negative Selection
    • 51:19 26.
      Schematic and Time Line
    • 52:05 27.
      Schematic and Time Line
    • 54:41 28.
      Comparison of Time line
    • 57:14 29.
      Conditional Knock-out
    • 1:00:49 30.
      Tissue-Specific Knock-out
    • 1:05:04 31.
      Conditional Knock-out(Time Specific)
    • 1:07:27 32.
      Mouse Strain Nomenclature
    • 1:09:51 33.
      Transgenic Mouse Strain Nomenclature
    • 1:10:40 34.
      A Laboratory Code
    • 1:11:27 35.
      Congenic Strain
    • 1:12:21 36.
      Knockout Mouse Strain Nomenclature
    • 1:14:20 37.
      Nude mice
    • 1:18:48 38.
      Severe Combined Immunodeficiency (SCID) Mice
    • 1:20:16 39.
      NSG Mouse
    • 1:23:15 40.
      NSG-SGM3 Mouse
    • 1:24:53 41.
      Humanize PDX mouse (擬人鼠)
    Location
    Folder name
    2021
    Author
    賴亮全
    Branch
    賴亮全教授
    Created
    2021-05-04 16:12:32
    Last Updated
    2021-05-05 13:19:07
    Duration
    1:26:00