• 00:00 1.
    Histone Mehtylation
  • 01:01 2.
    Histone Modification and Histone Codes
  • 01:51 3.
    Histone Acetylation
  • 03:54 4.
    Aberrant Histone Modifications in Cancer
  • 05:26 5.
    Locus-Specific Genetic Changes
  • 07:24 6.
    Locus-Specific Epigenetic Changes
  • 08:51 7.
    Epigenetic Mechanisms
  • 09:08 8.
    Polycomb–Trithorax Protein Complexes
  • 09:55 9.
    ATP-Based Chromatin-Remodeling Complexes
  • 10:38 10.
    Epigenetic Mechanisms
  • 10:43 11.
    PRION
  • 11:54 12.
    期末報告—互評
  • 13:11 13.
    Epigenetic Mechanisms
  • 13:24 14.
    Non-coding RNA
  • 14:48 15.
    Non-coding RNA classification
  • 15:19 16.
    Function of Non-coding RNA
  • 16:16 17.
    Number of HGNC Gene Symbols
  • 16:46 18.
    Nomenclature of rRNA
  • 18:31 19.
    Nomenclature of rRNA
  • 19:47 20.
    Nomenclature of tRNA
  • 21:00 21.
    Panoramic RNA display by overcoming RNA modification aborted sequencingA combinatorial enzymatic treatment to remove key RNA modifications AlkB enzyme: prevent blockage of reverse transcriptionT4PNK enzyme: prevent blockage of adapter ligation
  • 22:24 22.
    Systematically discovery of LncRNA
  • 24:33 23.
    Origins of LncRNAs
  • 25:33 24.
    Long Non-coding RNA Nomenclature
  • 27:13 25.
    Long Non-coding RNA Nomenclature
  • 28:30 26.
    LncRNA Functions
  • 31:04 27.
    Long non-coding RNAs as a Source of New Peptides
  • 33:32 28.
    Tools to predict translation potential of noncoding RNA
  • 34:11 29.
    dUTP Based Strand-Specific Sequencing
  • 35:45 30.
    dUTP Based Strand-Specific Sequencing
  • 36:41 31.
    Methods for Studying lncRNAs
  • 38:45 32.
    Methods for Detecting Protein-RNA Interactions
  • 39:18 33.
    RNase Protection Assay
  • 40:18 34.
    Electrophoretic Mobility Shift Assay (EMSA)
  • 42:40 35.
    RIP-Seq
  • 43:07 36.
    Question
  • 43:26 37.
    Protein A, G and L Antibody-Binding Ligands
  • 45:18 38.
    CLIP-Seq
  • 45:38 39.
    Crosslink Reagents
  • 46:41 40.
    RNA Pulldown Assay
  • 47:49 41.
    Aptamer
  • 50:03 42.
    RNA Fluorescent In-Situ Hybridiation (FISH)
  • 50:49 43.
    Prediction of Human lncRNA-Protein Interactions
  • 51:25 44.
    ENCORI
  • 52:06 45.
    RNA-Protein Interaction Prediction (RPISeq)
  • 52:58 46.
    Methods for Studying lncRNAs
  • 54:49 47.
    Summary graph
  • 55:50 48.
    Summary Table
  • 56:07 49.
    Methods for Studying lncRNAs
  • 56:25 50.
    Circular RNA
  • 57:27 51.
    Circular RNA
  • 58:55 52.
    Circular RNA
  • 1:00:20 53.
    Mechanisms of circRNA translation
  • 1:01:44 54.
    Functions of circRNA
  • 1:03:47 55.
    circRNA-Protein Interactions
  • 1:04:15 56.
    Circular RNA Interactome
  • 1:04:24 57.
    circRNA databases
  • 1:04:30 58.
    Post-Transcriptional Regulation
  • 1:05:41 59.
    microRNA1
  • 1:06:48 60.
    microRNA2
  • 1:07:34 61.
    microRNA3
  • 1:08:50 62.
    Biogenesis of microRNA
  • 1:12:50 63.
    miRNA Nomenclature
  • 1:15:37 64.
    Functions of miRNA
  • 1:15:59 65.
    How to research miRNA?
  • 1:16:12 66.
    Approaches to miRNA Discovery1
  • 1:17:22 67.
    Approaches to miRNA Discovery2
  • 1:18:54 68.
    Approaches to miRNA Discovery3
  • 1:19:15 69.
    Alternative Reverse Transcription Methodologies to Generate cDNA from miRNA
  • 1:19:59 70.
    cDNA synthesis by using stem-loop and linear miRNA-specific primers
  • 1:20:52 71.
    RT-PCR miRNA Assays
  • 1:21:57 72.
    Approaches to miRNA Discovery4
  • 1:22:30 73.
    Detection of microRNAs by In Situ Hybridization (ISH)
  • 1:23:44 74.
    Approaches to miRNA Discovery5
  • 1:24:42 75.
    How to research miRNA?
  • 1:24:49 76.
    Approaches to Predict miRNA Target
  • 1:26:01 77.
    List of Tools for miRNA Target Prediction
  • 1:26:20 78.
    Question
  • 1:26:29 79.
    miRSystem
  • 1:28:14 80.
    Luciferase Reporter Assay for MiRNA Target
  • 1:29:29 81.
    Analyses of miRNA Targets Using NGS
  • 1:29:51 82.
    How to research miRNA?
  • 1:29:58 83.
    Nanostring nCounter miRNA Assay
  • 1:30:54 84.
    AntimiRs
  • 1:31:16 85.
    Modified Nucleic Acid
  • 1:32:11 86.
    AntimiRs
  • 1:33:04 87.
    miR mimics
  • 1:33:53 88.
    Tools4miRs
  • 1:34:13 89.
    Competing Endogenous RNA (ceRNA)
  • 1:35:35 90.
    Discovery of RNA interference
  • 1:36:06 91.
    Silencing of unc-22 gene in C. elegans
  • 1:36:54 92.
    Short Interfering RNA (siRNA)
  • 1:38:27 93.
    Mechanism of siRNA
  • 1:39:23 94.
    General Design Guidelines for siRNA
  • 1:41:14 95.
    Off-Target Effects
  • 1:42:17 96.
    Appropriate Experimental Controls to Minimize Off-Target Effects (OTEs)
  • 1:43:55 97.
    siRNA Expression
  • 1:45:51 98.
    siRNA Delivery & Processing
  • 1:47:29 99.
    siRNA as Human Therapeutics
  • 1:48:01 100.
    Differences Between siRNA & miRNA
  • 1:49:30 101.
    Difference Between miRNA & siRNA
  • 1:50:13 102.
    Morpholino
  • 1:50:59 103.
    Summary
  • 1:51:55 104.
    Question
  • 1:52:36 105.
    Question
  • 1:52:46 106.
    Small Activating RNAs (saRNAs)
  • Index
  • Notes
  • Comment
  • Fullscreen
Epigenetics_noncoding RNA (0518)
Duration: 1:53:34, Browse: 757, Last Updated: 2021-05-18
    Prev Next
    • 00:00 1.
      Histone Mehtylation
    • 01:01 2.
      Histone Modification and Histone Codes
    • 01:51 3.
      Histone Acetylation
    • 03:54 4.
      Aberrant Histone Modifications in Cancer
    • 05:26 5.
      Locus-Specific Genetic Changes
    • 07:24 6.
      Locus-Specific Epigenetic Changes
    • 08:51 7.
      Epigenetic Mechanisms
    • 09:08 8.
      Polycomb–Trithorax Protein Complexes
    • 09:55 9.
      ATP-Based Chromatin-Remodeling Complexes
    • 10:38 10.
      Epigenetic Mechanisms
    • 10:43 11.
      PRION
    • 11:54 12.
      期末報告—互評
    • 13:11 13.
      Epigenetic Mechanisms
    • 13:24 14.
      Non-coding RNA
    • 14:48 15.
      Non-coding RNA classification
    • 15:19 16.
      Function of Non-coding RNA
    • 16:16 17.
      Number of HGNC Gene Symbols
    • 16:46 18.
      Nomenclature of rRNA
    • 18:31 19.
      Nomenclature of rRNA
    • 19:47 20.
      Nomenclature of tRNA
    • 21:00 21.
      Panoramic RNA display by overcoming RNA modification aborted sequencingA combinatorial enzymatic treatment to remove key RNA modifications AlkB enzyme: prevent blockage of reverse transcriptionT4PNK enzyme: prevent blockage of adapter ligation
    • 22:24 22.
      Systematically discovery of LncRNA
    • 24:33 23.
      Origins of LncRNAs
    • 25:33 24.
      Long Non-coding RNA Nomenclature
    • 27:13 25.
      Long Non-coding RNA Nomenclature
    • 28:30 26.
      LncRNA Functions
    • 31:04 27.
      Long non-coding RNAs as a Source of New Peptides
    • 33:32 28.
      Tools to predict translation potential of noncoding RNA
    • 34:11 29.
      dUTP Based Strand-Specific Sequencing
    • 35:45 30.
      dUTP Based Strand-Specific Sequencing
    • 36:41 31.
      Methods for Studying lncRNAs
    • 38:45 32.
      Methods for Detecting Protein-RNA Interactions
    • 39:18 33.
      RNase Protection Assay
    • 40:18 34.
      Electrophoretic Mobility Shift Assay (EMSA)
    • 42:40 35.
      RIP-Seq
    • 43:07 36.
      Question
    • 43:26 37.
      Protein A, G and L Antibody-Binding Ligands
    • 45:18 38.
      CLIP-Seq
    • 45:38 39.
      Crosslink Reagents
    • 46:41 40.
      RNA Pulldown Assay
    • 47:49 41.
      Aptamer
    • 50:03 42.
      RNA Fluorescent In-Situ Hybridiation (FISH)
    • 50:49 43.
      Prediction of Human lncRNA-Protein Interactions
    • 51:25 44.
      ENCORI
    • 52:06 45.
      RNA-Protein Interaction Prediction (RPISeq)
    • 52:58 46.
      Methods for Studying lncRNAs
    • 54:49 47.
      Summary graph
    • 55:50 48.
      Summary Table
    • 56:07 49.
      Methods for Studying lncRNAs
    • 56:25 50.
      Circular RNA
    • 57:27 51.
      Circular RNA
    • 58:55 52.
      Circular RNA
    • 1:00:20 53.
      Mechanisms of circRNA translation
    • 1:01:44 54.
      Functions of circRNA
    • 1:03:47 55.
      circRNA-Protein Interactions
    • 1:04:15 56.
      Circular RNA Interactome
    • 1:04:24 57.
      circRNA databases
    • 1:04:30 58.
      Post-Transcriptional Regulation
    • 1:05:41 59.
      microRNA1
    • 1:06:48 60.
      microRNA2
    • 1:07:34 61.
      microRNA3
    • 1:08:50 62.
      Biogenesis of microRNA
    • 1:12:50 63.
      miRNA Nomenclature
    • 1:15:37 64.
      Functions of miRNA
    • 1:15:59 65.
      How to research miRNA?
    • 1:16:12 66.
      Approaches to miRNA Discovery1
    • 1:17:22 67.
      Approaches to miRNA Discovery2
    • 1:18:54 68.
      Approaches to miRNA Discovery3
    • 1:19:15 69.
      Alternative Reverse Transcription Methodologies to Generate cDNA from miRNA
    • 1:19:59 70.
      cDNA synthesis by using stem-loop and linear miRNA-specific primers
    • 1:20:52 71.
      RT-PCR miRNA Assays
    • 1:21:57 72.
      Approaches to miRNA Discovery4
    • 1:22:30 73.
      Detection of microRNAs by In Situ Hybridization (ISH)
    • 1:23:44 74.
      Approaches to miRNA Discovery5
    • 1:24:42 75.
      How to research miRNA?
    • 1:24:49 76.
      Approaches to Predict miRNA Target
    • 1:26:01 77.
      List of Tools for miRNA Target Prediction
    • 1:26:20 78.
      Question
    • 1:26:29 79.
      miRSystem
    • 1:28:14 80.
      Luciferase Reporter Assay for MiRNA Target
    • 1:29:29 81.
      Analyses of miRNA Targets Using NGS
    • 1:29:51 82.
      How to research miRNA?
    • 1:29:58 83.
      Nanostring nCounter miRNA Assay
    • 1:30:54 84.
      AntimiRs
    • 1:31:16 85.
      Modified Nucleic Acid
    • 1:32:11 86.
      AntimiRs
    • 1:33:04 87.
      miR mimics
    • 1:33:53 88.
      Tools4miRs
    • 1:34:13 89.
      Competing Endogenous RNA (ceRNA)
    • 1:35:35 90.
      Discovery of RNA interference
    • 1:36:06 91.
      Silencing of unc-22 gene in C. elegans
    • 1:36:54 92.
      Short Interfering RNA (siRNA)
    • 1:38:27 93.
      Mechanism of siRNA
    • 1:39:23 94.
      General Design Guidelines for siRNA
    • 1:41:14 95.
      Off-Target Effects
    • 1:42:17 96.
      Appropriate Experimental Controls to Minimize Off-Target Effects (OTEs)
    • 1:43:55 97.
      siRNA Expression
    • 1:45:51 98.
      siRNA Delivery & Processing
    • 1:47:29 99.
      siRNA as Human Therapeutics
    • 1:48:01 100.
      Differences Between siRNA & miRNA
    • 1:49:30 101.
      Difference Between miRNA & siRNA
    • 1:50:13 102.
      Morpholino
    • 1:50:59 103.
      Summary
    • 1:51:55 104.
      Question
    • 1:52:36 105.
      Question
    • 1:52:46 106.
      Small Activating RNAs (saRNAs)
    Location
    1. Media Center
    2. ...
    3. 2021
    Folder name
    2021
    Author
    賴亮全
    Branch
    賴亮全教授
    Created
    2021-05-18 14:11:47
    Last Updated
    2021-05-18 19:37:13
    Duration
    1:53:34

    臺北市仁愛路一段1號10樓1009室

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    E-mail: llai@ntu.edu.tw

    Tel: 886-2-2312-3456 # 288241

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