Protein interaction (0525) | 賴亮全實驗室

索引

00:00 1.
DNA-Protein interaction
00:08 2.
Interaction Between Proteins and DNA
01:22 3.
Chromatin Immunoprecipitation (ChIP)
03:41 4.
Antigen vs Antibody
06:16 5.
Antibody Structure & Isotype
07:33 6.
Polyclonal Antibody: antibody preparations from immunized animals contain different antibodies produced by many B cell clonesMonoclonal Antibody:homogeneous antibody preparations produced in laboratoryconsist of a single type of antigen binding site (sing
08:15 7.
How to make monoclonal antibodies?
13:22 8.
Monoclonal Recombinant antibody
14:34 9.
Nomenclature of Antibody
15:55 10.
How to make phosphospecific antibodies?
17:44 11.
Question
17:54 12.
Protein A, G and L Antibody-Binding Ligands
18:47 13.
ChIP-PCR/qPCR
19:55 14.
ChIP on chip
21:04 15.
ChIP-Seq
22:58 16.
ATAC-Seq
23:58 17.
ChIP-seq vs ATAC-seq
24:32 18.
DNase Seq
25:08 19.
MNase-seq
26:41 20.
FAIRE-seq
27:37 21.
Chromatin Structure Analyses
28:49 22.
Transposase-Accessible Chromatin with Visualization (ATAC-see)
29:39 23.
Luciferase Reporter Assay for mRNA Promoter
32:01 24.
Electrophoretic Mobility Shift Assay (EMSA)
34:21 25.
DNA Footprinting Assay
35:28 26.
DNA Pull-Down Assay
36:31 27.
Mass Spectrometry
36:53 28.
Tandem Mass Spectrometry
37:17 29.
enChIP
38:32 30.
ENCODEEncyclopedia of DNA Elements
40:28 31.
Applications: Protein-protein interaction
40:34 32.
Protein Tag
43:13 33.
GST (glutathione S-transferase) pull-down assay: a technique to test interaction between a tagged protein or the bait (GST, His6, biotin ...) and another protein (test protein, or prey)
44:10 34.
Immunoprecipitation
45:01 35.
Western Blotting
47:43 36.
Consideration of Choosing Antibodies
49:55 37.
Protein Position is different from what is expected
50:56 38.
Immunohistochemistry (IHC)
52:09 39.
Multiplexed protein detection (up to 96 proteins) on FFPE tissue
54:20 40.
Used to discover protein-protein interactions by testing for physical interactions between two proteinsThe transcription factor is split into two separate fragments: The BD (binding domain): binding to the upstream activating sequence (UAS)Fused with bait

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討論

00:00 1.
DNA-Protein interaction
00:08 2.
Interaction Between Proteins and DNA
01:22 3.
Chromatin Immunoprecipitation (ChIP)
03:41 4.
Antigen vs Antibody
06:16 5.
Antibody Structure & Isotype
07:33 6.
Polyclonal Antibody: antibody preparations from immunized animals contain different antibodies produced by many B cell clonesMonoclonal Antibody:homogeneous antibody preparations produced in laboratoryconsist of a single type of antigen binding site (sing
08:15 7.
How to make monoclonal antibodies?
13:22 8.
Monoclonal Recombinant antibody
14:34 9.
Nomenclature of Antibody
15:55 10.
How to make phosphospecific antibodies?
17:44 11.
Question
17:54 12.
Protein A, G and L Antibody-Binding Ligands
18:47 13.
ChIP-PCR/qPCR
19:55 14.
ChIP on chip
21:04 15.
ChIP-Seq
22:58 16.
ATAC-Seq
23:58 17.
ChIP-seq vs ATAC-seq
24:32 18.
DNase Seq
25:08 19.
MNase-seq
26:41 20.
FAIRE-seq
27:37 21.
Chromatin Structure Analyses
28:49 22.
Transposase-Accessible Chromatin with Visualization (ATAC-see)
29:39 23.
Luciferase Reporter Assay for mRNA Promoter
32:01 24.
Electrophoretic Mobility Shift Assay (EMSA)
34:21 25.
DNA Footprinting Assay
35:28 26.
DNA Pull-Down Assay
36:31 27.
Mass Spectrometry
36:53 28.
Tandem Mass Spectrometry
37:17 29.
enChIP
38:32 30.
ENCODEEncyclopedia of DNA Elements
40:28 31.
Applications: Protein-protein interaction
40:34 32.
Protein Tag
43:13 33.
GST (glutathione S-transferase) pull-down assay: a technique to test interaction between a tagged protein or the bait (GST, His6, biotin ...) and another protein (test protein, or prey)
44:10 34.
Immunoprecipitation
45:01 35.
Western Blotting
47:43 36.
Consideration of Choosing Antibodies
49:55 37.
Protein Position is different from what is expected
50:56 38.
Immunohistochemistry (IHC)
52:09 39.
Multiplexed protein detection (up to 96 proteins) on FFPE tissue
54:20 40.
Used to discover protein-protein interactions by testing for physical interactions between two proteinsThe transcription factor is split into two separate fragments: The BD (binding domain): binding to the upstream activating sequence (UAS)Fused with bait

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Protein interaction (0525)

長度: 56:34, 瀏覽: 831, 最近修訂: 2021-05-24

00:00 1.
DNA-Protein interaction
00:08 2.
Interaction Between Proteins and DNA
01:22 3.
Chromatin Immunoprecipitation (ChIP)
03:41 4.
Antigen vs Antibody
06:16 5.
Antibody Structure & Isotype
07:33 6.
Polyclonal Antibody: antibody preparations from immunized animals contain different antibodies produced by many B cell clonesMonoclonal Antibody:homogeneous antibody preparations produced in laboratoryconsist of a single type of antigen binding site (sing
08:15 7.
How to make monoclonal antibodies?
13:22 8.
Monoclonal Recombinant antibody
14:34 9.
Nomenclature of Antibody
15:55 10.
How to make phosphospecific antibodies?
17:44 11.
Question
17:54 12.
Protein A, G and L Antibody-Binding Ligands
18:47 13.
ChIP-PCR/qPCR
19:55 14.
ChIP on chip
21:04 15.
ChIP-Seq
22:58 16.
ATAC-Seq
23:58 17.
ChIP-seq vs ATAC-seq
24:32 18.
DNase Seq
25:08 19.
MNase-seq
26:41 20.
FAIRE-seq
27:37 21.
Chromatin Structure Analyses
28:49 22.
Transposase-Accessible Chromatin with Visualization (ATAC-see)
29:39 23.
Luciferase Reporter Assay for mRNA Promoter
32:01 24.
Electrophoretic Mobility Shift Assay (EMSA)
34:21 25.
DNA Footprinting Assay
35:28 26.
DNA Pull-Down Assay
36:31 27.
Mass Spectrometry
36:53 28.
Tandem Mass Spectrometry
37:17 29.
enChIP
38:32 30.
ENCODEEncyclopedia of DNA Elements
40:28 31.
Applications: Protein-protein interaction
40:34 32.
Protein Tag
43:13 33.
GST (glutathione S-transferase) pull-down assay: a technique to test interaction between a tagged protein or the bait (GST, His6, biotin ...) and another protein (test protein, or prey)
44:10 34.
Immunoprecipitation
45:01 35.
Western Blotting
47:43 36.
Consideration of Choosing Antibodies
49:55 37.
Protein Position is different from what is expected
50:56 38.
Immunohistochemistry (IHC)
52:09 39.
Multiplexed protein detection (up to 96 proteins) on FFPE tissue
54:20 40.
Used to discover protein-protein interactions by testing for physical interactions between two proteinsThe transcription factor is split into two separate fragments: The BD (binding domain): binding to the upstream activating sequence (UAS)Fused with bait

位置

資料夾名稱

2021

發表人

賴亮全

單位

賴亮全教授

建立

2021-05-24 14:48:08

最近修訂

2021-05-24 17:25:35

長度

56:34