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00:08
1.
DNA-Protein interaction
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01:13
2.
Interaction Between Proteins and DNA
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02:19
3.
Chromatin Immunoprecipitation (ChIP)
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02:35
4.
Antigen vs Antibody
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01:16
5.
Antibody Structure & Isotype
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00:41
6.
Polyclonal Antibody: antibody preparations from immunized animals contain different antibodies produced by many B cell clonesMonoclonal Antibody:homogeneous antibody preparations produced in laboratoryconsist of a single type of antigen binding site (sing
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05:07
7.
How to make monoclonal antibodies?
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01:12
8.
Monoclonal Recombinant antibody
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01:20
9.
Nomenclature of Antibody
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01:48
10.
How to make phosphospecific antibodies?
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00:09
11.
Question
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00:53
12.
Protein A, G and L Antibody-Binding Ligands
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01:07
13.
ChIP-PCR/qPCR
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01:09
14.
ChIP on chip
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01:54
15.
ChIP-Seq
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00:59
16.
ATAC-Seq
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00:34
17.
ChIP-seq vs ATAC-seq
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00:35
18.
DNase Seq
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01:33
19.
MNase-seq
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00:55
20.
FAIRE-seq
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01:11
21.
Chromatin Structure Analyses
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00:50
22.
Transposase-Accessible Chromatin with Visualization (ATAC-see)
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02:21
23.
Luciferase Reporter Assay for mRNA Promoter
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02:20
24.
Electrophoretic Mobility Shift Assay (EMSA)
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01:07
25.
DNA Footprinting Assay
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01:02
26.
DNA Pull-Down Assay
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00:22
27.
Mass Spectrometry
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00:23
28.
Tandem Mass Spectrometry
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01:15
29.
enChIP
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01:56
30.
ENCODEEncyclopedia of DNA Elements
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00:05
31.
Applications: Protein-protein interaction
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02:38
32.
Protein Tag
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00:57
33.
GST (glutathione S-transferase) pull-down assay: a technique to test interaction between a tagged protein or the bait (GST, His6, biotin ...) and another protein (test protein, or prey)
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00:50
34.
Immunoprecipitation
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02:42
35.
Western Blotting
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02:12
36.
Consideration of Choosing Antibodies
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01:00
37.
Protein Position is different from what is expected
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01:13
38.
Immunohistochemistry (IHC)
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02:10
39.
Multiplexed protein detection (up to 96 proteins) on FFPE tissue
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02:13
40.
Used to discover protein-protein interactions by testing for physical interactions between two proteinsThe transcription factor is split into two separate fragments: The BD (binding domain): binding to the upstream activating sequence (UAS)Fused with bait