• 00:00 1.
    DNA-Protein interaction
  • 00:08 2.
    Interaction Between Proteins and DNA
  • 01:22 3.
    Chromatin Immunoprecipitation (ChIP)
  • 03:41 4.
    Antigen vs Antibody
  • 06:16 5.
    Antibody Structure & Isotype
  • 07:33 6.
    Polyclonal Antibody: antibody preparations from immunized animals contain different antibodies produced by many B cell clonesMonoclonal Antibody:homogeneous antibody preparations produced in laboratoryconsist of a single type of antigen binding site (sing
  • 08:15 7.
    How to make monoclonal antibodies?
  • 13:22 8.
    Monoclonal Recombinant antibody
  • 14:34 9.
    Nomenclature of Antibody
  • 15:55 10.
    How to make phosphospecific antibodies?
  • 17:44 11.
    Question
  • 17:54 12.
    Protein A, G and L Antibody-Binding Ligands
  • 18:47 13.
    ChIP-PCR/qPCR
  • 19:55 14.
    ChIP on chip
  • 21:04 15.
    ChIP-Seq
  • 22:58 16.
    ATAC-Seq
  • 23:58 17.
    ChIP-seq vs ATAC-seq
  • 24:32 18.
    DNase Seq
  • 25:08 19.
    MNase-seq
  • 26:41 20.
    FAIRE-seq
  • 27:37 21.
    Chromatin Structure Analyses
  • 28:49 22.
    Transposase-Accessible Chromatin with Visualization (ATAC-see)
  • 29:39 23.
    Luciferase Reporter Assay for mRNA Promoter
  • 32:01 24.
    Electrophoretic Mobility Shift Assay (EMSA)
  • 34:21 25.
    DNA Footprinting Assay
  • 35:28 26.
    DNA Pull-Down Assay
  • 36:31 27.
    Mass Spectrometry
  • 36:53 28.
    Tandem Mass Spectrometry
  • 37:17 29.
    enChIP
  • 38:32 30.
    ENCODEEncyclopedia of DNA Elements
  • 40:28 31.
    Applications: Protein-protein interaction
  • 40:34 32.
    Protein Tag
  • 43:13 33.
    GST (glutathione S-transferase) pull-down assay: a technique to test interaction between a tagged protein or the bait (GST, His6, biotin ...) and another protein (test protein, or prey)
  • 44:10 34.
    Immunoprecipitation
  • 45:01 35.
    Western Blotting
  • 47:43 36.
    Consideration of Choosing Antibodies
  • 49:55 37.
    Protein Position is different from what is expected
  • 50:56 38.
    Immunohistochemistry (IHC)
  • 52:09 39.
    Multiplexed protein detection (up to 96 proteins) on FFPE tissue
  • 54:20 40.
    Used to discover protein-protein interactions by testing for physical interactions between two proteinsThe transcription factor is split into two separate fragments: The BD (binding domain): binding to the upstream activating sequence (UAS)Fused with bait
  • Index
  • Notes
  • Comment
  • Fullscreen
Protein interaction (0525)
Duration: 56:34, Browse: 503, Last Updated: 2021-05-24
    • 00:00 1.
      DNA-Protein interaction
    • 00:08 2.
      Interaction Between Proteins and DNA
    • 01:22 3.
      Chromatin Immunoprecipitation (ChIP)
    • 03:41 4.
      Antigen vs Antibody
    • 06:16 5.
      Antibody Structure & Isotype
    • 07:33 6.
      Polyclonal Antibody: antibody preparations from immunized animals contain different antibodies produced by many B cell clonesMonoclonal Antibody:homogeneous antibody preparations produced in laboratoryconsist of a single type of antigen binding site (sing
    • 08:15 7.
      How to make monoclonal antibodies?
    • 13:22 8.
      Monoclonal Recombinant antibody
    • 14:34 9.
      Nomenclature of Antibody
    • 15:55 10.
      How to make phosphospecific antibodies?
    • 17:44 11.
      Question
    • 17:54 12.
      Protein A, G and L Antibody-Binding Ligands
    • 18:47 13.
      ChIP-PCR/qPCR
    • 19:55 14.
      ChIP on chip
    • 21:04 15.
      ChIP-Seq
    • 22:58 16.
      ATAC-Seq
    • 23:58 17.
      ChIP-seq vs ATAC-seq
    • 24:32 18.
      DNase Seq
    • 25:08 19.
      MNase-seq
    • 26:41 20.
      FAIRE-seq
    • 27:37 21.
      Chromatin Structure Analyses
    • 28:49 22.
      Transposase-Accessible Chromatin with Visualization (ATAC-see)
    • 29:39 23.
      Luciferase Reporter Assay for mRNA Promoter
    • 32:01 24.
      Electrophoretic Mobility Shift Assay (EMSA)
    • 34:21 25.
      DNA Footprinting Assay
    • 35:28 26.
      DNA Pull-Down Assay
    • 36:31 27.
      Mass Spectrometry
    • 36:53 28.
      Tandem Mass Spectrometry
    • 37:17 29.
      enChIP
    • 38:32 30.
      ENCODEEncyclopedia of DNA Elements
    • 40:28 31.
      Applications: Protein-protein interaction
    • 40:34 32.
      Protein Tag
    • 43:13 33.
      GST (glutathione S-transferase) pull-down assay: a technique to test interaction between a tagged protein or the bait (GST, His6, biotin ...) and another protein (test protein, or prey)
    • 44:10 34.
      Immunoprecipitation
    • 45:01 35.
      Western Blotting
    • 47:43 36.
      Consideration of Choosing Antibodies
    • 49:55 37.
      Protein Position is different from what is expected
    • 50:56 38.
      Immunohistochemistry (IHC)
    • 52:09 39.
      Multiplexed protein detection (up to 96 proteins) on FFPE tissue
    • 54:20 40.
      Used to discover protein-protein interactions by testing for physical interactions between two proteinsThe transcription factor is split into two separate fragments: The BD (binding domain): binding to the upstream activating sequence (UAS)Fused with bait
    Location
    Folder name
    2021
    Author
    賴亮全
    Branch
    賴亮全教授
    Created
    2021-05-24 14:48:08
    Last Updated
    2021-05-24 17:25:35
    Duration
    56:34