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賴亮全實驗室
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系統教學
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Bioinfo Teams連結
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細胞培養 (Cell culture)
(4)
Sterile Cell Culture Technique 細胞培養無菌技術
Cell Culture Training Video (細胞培養)
How to subculture (passage) primary cells
Counting Cells with a Hemocytometer
Cell Culture in Hypoxia Chamber
(1)
hypoxia chamber 使用SOP
Cell Freeze
(3)
凍細胞
凍細胞_demo
Freezing Cells: Cell Culture Basics
DNA/RNA extraction
(3)
Protocol 1 - DNA Extraction Part 1
Protocol 1 - DNA Extraction Part 2
RNA Extraction by TRIzol®
Nuclear and Cytoplasmic Protein Extraction
(1)
Nuclear and Cytoplasmic Protein Extraction
Transfection (轉染)
(1)
Easy DNA transfection with jetPRIME - Polyplus-transfection
設計引子SOP
(1)
設計primer流程 (捷登)
自己動手microRNA引物設計
(1)
自己動手microRNA引物設計
引子訂購流程SOP
(1)
訂購primer流程
Real time PCR (Abnova版本)
(1)
Real-time PCR
一共14樓qPCR-ABI7900上機SOP
(1)
14樓qPCR-ABI7000上機SOP
PCR
(3)
Protocol 2 - PCR Part 1
Protocol 2 - PCR Part 2
EX_LNAanimation
circular RNA 相關實驗 SOP
(2)
circRNA SOP
捷登circRNA交接報告
設計及訂購primer
(1)
Pimer Design and order
Making DNA constructs
(14)
protocols
Simply Cloning - Introduction
Simply Cloning - Chapter 1 - Planning
Simply Cloning - Chapter 2 - PCR
Simply Cloning - Chapter 3 - Vector Restriction Digest
Simply Cloning - Chapter 4 - Gel Purification
Simply Cloning - Chapter 5 - Insert Restriction Digest
Simply Cloning - Chapter 7 - Transformation
Simply Cloning - Chapter 8 - Plasmid Miniprep
Simply Cloning - Chapter 9 - Clone Verification
[More]
Simply Cloning - Chapter 10 - Clone Library
Simply Cloning - Supplement 1 - Troubleshooting
Simply Cloning - Supplement 2 - Cracking Gel
Simply Cloning - Supplement 3 - pDRAW32
質體(plasmid/vector)清單
(0)
索取質體SOP
(1)
索取質體SOP
Restriction Digest
(1)
Protocol 3 - Restriction Digest
Gel Electrophoresis (電泳)
(2)
Protocol 4 - Gel Electrophoresis
DNA gel electrophoresis (VR360)
Transformation / Transfection
(1)
transfection
Clone製作 (品豪)
(2)
20190114專題報告-clone製作初級版108年
20190121專題報告-clone製作進階版108年
Western blot introduction
(1)
Western Blotting
Western blot (Genetex版本)
(3)
Western Blot - 1_Sample preparation [Video from GeneTex]
Western Blot - 2_Electrophoresis [Video from GeneTex]
Western Blot - 3_Semi-Dry transfer [Video from GeneTex]
Western blot (冠儀版本)
(1)
western blot
Protein stability and storage (Pierce版本)
(1)
Protein stability and storage
Clonogenic assay (Abnova版本)
(1)
Clonogenic Assay
migration assay (Abnova版本; 伊純)
(1)
Migration Assay
Invasion assay (Abnova 版本)
(1)
Invasion Assay
Flowcytometry 技術
(6)
Flowcytometry (品豪)
Molecular Probes Tutorial Series— Anatomy of Fluorescence Spectra
Molecular Probes Tutorial Series—Analyzing Flow Cytometry Data
Molecular Probes Tutorial Series—Introduction to Flow Cytometry
Molecular Probes Tutorial Series—Introduction to Fluorescence
Molecular Probes Tutorial Series—Overview of Filters and Light Sources
MTT Assay (BCRC版本)
(1)
MTT Assay
MTT assay(忠賢)
(3)
MTT
1. 加入MTT
2. 加入DMSO
MTT you tube 鈺琴
(1)
MTT Assay
MTT assay (薏陵)
(2)
MTT protocol 薏陵版本
MTT assay操作步驟
RNA Pulldown
(1)
RNA PULL DOWN1
IP (immunoprecipitation) 捷登
(1)
How to use Dynabeads® for immunoprecipitation
Immunohistochemistry (免疫組織染色)
(2)
Immunohistochemistry
Fluorescence In Situ Hybridization (FISH)
Fluorescence In Situ Hybridization (FISH)
(1)
Fluorescence In Situ Hybridization (FISH)
RNA immunoprecipitation (RIP)
(2)
CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells | Protocol
RIP
動物實驗
(7)
皮下注射腫瘤
尾靜脈注射
取腫瘤
IVIS Spectrum 中文操作 LI4.x
IVIS上
IVIS下
Tail vain
正立共軛焦顯微鏡LSM 880操作示範
(3)
正立共軛焦顯微鏡LSM 880操作示範part1_LSM880_1
共軛焦顯微鏡關機教學_LSM880_3
正立共軛焦顯微鏡LSM 880操作示範part2_LSM880_2 ZEN2
螢光顯微鏡M1教學影片
(9)
9.關機
8.@DIC拍攝重點提示
7.@存檔及轉檔
6.@拍螢光
5.#ZEN軟體操作 2 拍攝彩色影像大型拼圖
4.#ZEN軟體 1拍攝彩色影像
3.#穿透光明視野操作示範
2.顯微鏡介紹
1. Axio Imager 開機
染色影片
(1)
RNAscope 染色影片
瓦斯本生燈使用注意事項
(1)
Rocker 200220 電子點火式本生燈 操作影片
呼吸生理實驗
(3)
呼吸生理
呼吸生理Power lab 操作示範
保鮮膜增加阻力
觸控螢幕
(7)
盛源小學堂-電子白板-基本操作
盛源小學堂-如何設定與叫出羅盤
盛源小學堂-如何使用批註操作
盛源小學堂-電子白板-單點.多點觸控設定
盛源小學堂-如何設定手機無線投影
盛源小學堂-如何設定筆電無線投影
盛源小學堂-如何操作無線投影-反向傳屏